EMAGE mouse gene expression spatial database

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EMAGE:18181

Arhgap27 Rho GTPase activating protein 27 ( MGI:1916903)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011913. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011913_01	euxassay_011913_02	euxassay_011913_03	euxassay_011913_04

euxassay_011913_05	euxassay_011913_06	euxassay_011913_07	euxassay_011913_08	euxassay_011913_09

euxassay_011913_10	euxassay_011913_11	euxassay_011913_12	euxassay_011913_13	euxassay_011913_14

euxassay_011913_15	euxassay_011913_16	euxassay_011913_17	euxassay_011913_18	euxassay_011913_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18181_wholemount_strong.wlz

18181_wholemount_moderate.wlz

18181_wholemount_weak.wlz

18181_wholemount_possible.wlz

18181_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18181_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 05 06 07 08 18 19 weak expression: see section 03 04 09 11 12 13 14 15 16 17

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 17 18 19

fibula

moderate

homogeneous

moderate expression: see section 03 04 05 06 07

tibia

moderate

homogeneous

moderate expression: see section 03 04 05 06

femur

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 17 18 19

otic capsule

moderate

homogeneous

moderate expression: see section 06 07 13 14 15

nasal septum

moderate

homogeneous

moderate expression: see section 10

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 14 15 weak expression: see section 02 03 04

axial skeleton

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13

basioccipital bone

moderate

homogeneous

moderate expression: see section 03 04 05 06 14 15 16 17 18 19

basisphenoid bone

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 16 17 18 19

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 05 06 15 16 17 18 19

scapula

moderate

homogeneous

moderate expression: see section 01 02 03 17

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 09 10 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30052
Entity Detected:	Arhgap27, Rho GTPase activating protein 27 ( MGI:1916903)
Sequence:	sense strand is shown >T30052 GATGCAGGCCTCCTGAGAACTTTTCCGATGCTTCCCTCTCTCCTTGGGCCACATCCGCTCTCTACAGACG TTCTTCATACTAACCACATACCAAACCTGGCAATCTGGGGCTTGCAGAAGTCACAGTACTTCAGGGGTCC ATAGGTGATCTGATTCCCTGCAAAGGCTATCCCCACAGCAAGCCCAGCTTCTAGAAGAGGGAGTCACGCC CCTGCCCCACTGCAGCACAGCCTGTAGGTAGACCAGAGTAAATGCTGGAGCACTTCGTGTAGGTTAGAGG TTGGGGGCCCCCCGAAGGCAGCAGGGAACCTACCACAGCTGTTTGGGGGGGCAGCATGAATAGAAGCCTC CAGCTGCTGTCTACACCCTGGCTGGGTGCCTACCTAAAGCCCCCAACCACTGTCTACATCCTGGCTGGCT GGATGCCTACCTGGTACCCACTTGCCATGCAGAGGCCTGTCCGGGAGGTCACAGCCTTAGGCAAACAGTT GAACCTGTGTCAACTGCCCGCTTCCTCCCTCGTGCACCTCAATCTATGGATCTGAGCCCCCAGCTCCACC CACCCCCACTGACTCAACAAACAATAATAGCTAATATTTATTGAGCATTCAGACTATGCCAGCCTTTCCC ACAGGCTTCTCCCTTGGTTTCATGCAGTCCATATTTATTGTGGGTCCTCATGTGCCTGGCACTGTGCCCT GGGGCAGGGGTTCTACGCATGCGTTCTGGAGCTTGGGGCTCCCAGTGAGTACGGCTGATGCAAGCCCAGC CCTGATGGTAGCAGGGCGCAGAAGAGAACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3964450), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 37280. Forward Primer - name:037280_F_IRAV20-23_A02_2310069I04Rik, sequence:GATGCAGGCCTCCTGAGA; Reverse Primer - name:037280_R_SP6_IRAV20-23_A02_2310069I04Rik, sequence:TTGTTCTCTTCTGCGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18183 same embryo
	EMAGE:18185 same embryo
	EMAGE:18184 same embryo
	EMAGE:18182 same embryo
	EMAGE:18180 same embryo
	EurExpress:euxassay_011913 same experiment
	MGI:4823223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source