EMAGE mouse gene expression spatial database

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EMAGE:18182

Pop4 processing of precursor 4, ribonuclease P/MRP family, (S. cerevisiae) ( MGI:1913411)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011921. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011921_01	euxassay_011921_02	euxassay_011921_03	euxassay_011921_04

euxassay_011921_05	euxassay_011921_06	euxassay_011921_07	euxassay_011921_08	euxassay_011921_09

euxassay_011921_10	euxassay_011921_11	euxassay_011921_12	euxassay_011921_13	euxassay_011921_14

euxassay_011921_15	euxassay_011921_16	euxassay_011921_17	euxassay_011921_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18182_wholemount_strong.wlz

18182_wholemount_moderate.wlz

18182_wholemount_weak.wlz

18182_wholemount_possible.wlz

18182_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18

radius

moderate

homogeneous

moderate expression: see section 18

humerus

moderate

homogeneous

moderate expression: see section 01 02 15 16 17 18

fibula

moderate

homogeneous

moderate expression: see section 03 04 05 06

tibia

moderate

homogeneous

moderate expression: see section 03 04 05 06

femur

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 16 17 18

otic capsule

moderate

homogeneous

moderate expression: see section 05 06 11 12

naris

moderate

homogeneous

moderate expression: see section 08

nasal septum

moderate

homogeneous

moderate expression: see section 09

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17

axial skeleton

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 15

basisphenoid bone

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 14 15 16 17 18

vault of skull

moderate

homogeneous

moderate expression: see section 01 02

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 14 15 16 17 18

scapula

moderate

homogeneous

moderate expression: see section 01 02 15 16

sternum

moderate

homogeneous

moderate expression: see section 09

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 07 08 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30055
Entity Detected:	Pop4, processing of precursor 4, ribonuclease P/MRP family, (S. cerevisiae) ( MGI:1913411)
Sequence:	sense strand is shown >T30055 AGCTGGCCGACAATGAAGGCTGCCATCTACCATGCCTTTTCTCATAAAGAAGCCAAGGACCACGATGTGC AGGAGCTGGGAAGCCAGCGGGCTGAGGCCTTTGTGCGGGCCTTCCTGAAGCAGAGCATACCTCACATGAG CCAAGAAGACTGTGAGAGCCATCTGCAGCGCAAGGCCGTGATCTTGGAATATTTCACTCGCCTAAAGCCA AGGCCAAGGCCAAAGAAGAAATCCAAAGGCCTCTCTGCCAAACAGAGGAGAGACATGCGGCTCTTTGACA TTAAACCAGAGCAACAGAGATACAGCCTGTTCCTCCCGCTCCATGAACTCTGGAAACAGTACATCCGGGA CCTGTGCAATGGGCTCAAGCCGGACACGCAGCCACAGATGATTCAGGCCAAGCTCCTAAAGGCAGATCTT CATGGTGCTATTATTTCAGTCACGAAATCCAAGTGCCCCTCCTATGTGGGAGTTACAGGAATCCTTCTGC AGGAGACCAAGCATGTCTTTAAGATCATCACCAGAGAAGACCACCTAAAAGTCATCCCCAAGCTGAATTG TGTGTTCACCATAGAAATTGATGACTTCATTTCCTACATTTATGGAAGCAAATTCCAGCTTCGGGCAAGT GAGCGGTCTGCCAAGAAGTTCAAAGCAAAGGGATCAATTGACCTGTGAACTCTGCCACTGGAGACAGATA TCCCTGACACCGAAGAACTGGAAACTCCCTAAATACTCACTTTTTCTTGAAGAGCCTCTGGCTGTAGCCC GTCTCTAGTCCAGGGAAAACAGAGGGGCTGAAGAGCATTGAACAACGCAGGAGAAAGCTAGGAGTAAATA TTTGTATTGTTAAGTGACCTTAAGTTAGCTTTAGTTCCACAGGGAGGAAAGACTAGCAGAAATACATCAG GATTAACTTTGGATCATCTGAAGAAGGTGGCATTTAGGCTCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3966371), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15530. Forward Primer - name:015530_F_IRAV20-23_A18_Pop4, sequence:AGCTGGCCGACAATGAAG; Reverse Primer - name:015530_R_SP6_IRAV20-23_A18_Pop4, sequence:GGCAGAGCCTAAATGCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18181 same embryo
	EMAGE:18183 same embryo
	EMAGE:18185 same embryo
	EMAGE:18184 same embryo
	EMAGE:18180 same embryo
	EurExpress:euxassay_011921 same experiment
	MGI:4827332 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source