EMAGE mouse gene expression spatial database

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EMAGE:18183

Atp6v0a2 ATPase, H+ transporting, lysosomal V0 subunit A2 ( MGI:104855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011914. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011914_01	euxassay_011914_02	euxassay_011914_03	euxassay_011914_04

euxassay_011914_05	euxassay_011914_06	euxassay_011914_07	euxassay_011914_08	euxassay_011914_09

euxassay_011914_10	euxassay_011914_11	euxassay_011914_12	euxassay_011914_13	euxassay_011914_14

euxassay_011914_15	euxassay_011914_16	euxassay_011914_17	euxassay_011914_18	euxassay_011914_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18183_wholemount_strong.wlz

18183_wholemount_moderate.wlz

18183_wholemount_weak.wlz

18183_wholemount_possible.wlz

18183_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18183_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 04 16 17 18 19

fibula

moderate

homogeneous

moderate expression: see section 04 05 06

tibia

moderate

homogeneous

moderate expression: see section 04 05 06

femur

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 16 17 18 19

otic capsule

moderate

homogeneous

moderate expression: see section 05 06 07 12 13 14

naris

moderate

homogeneous

moderate expression: see section 08 09 11 12

nasal septum

moderate

homogeneous

moderate expression: see section 10

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

axial skeleton

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

basioccipital bone

moderate

homogeneous

moderate expression: see section 05 06 07 14 16 17 18 19

basisphenoid bone

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 15

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 15 16 17 18 19

vault of skull

moderate

homogeneous

moderate expression: see section 01 02 03 04 05

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 14 15 16 17 18 19

scapula

moderate

homogeneous

moderate expression: see section 01 02 17

sternum

moderate

homogeneous

moderate expression: see section 10

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30054
Entity Detected:	Atp6v0a2, ATPase, H+ transporting, lysosomal V0 subunit A2 ( MGI:104855)
Sequence:	sense strand is shown >T30054 GCAGAAGCTGGAGGTGGAGCTCAGAGAAGTCACCAAGAACAAGGAGAAGCTGAGGAAGAACCTGTTGGAG CTGGTGGAGTACACCCACATGCTGAGGGTCACGAAGACCTTCCTCAAGCGGAATGTGGAGTTTGAGCCCA CGTATGAAGAGTTTCCCGCCTTAGAGAACGACTCTCTGTTGGACTACAGCTGCATGCAGCGGCTGGGCGC CAAGCTGGGATTCGTTTCTGGCCTGATTCAGCAAGGCAGAGTTGAAGCATTTGAAAGGATGCTGTGGCGG GCCTGCAAAGGGTACACCATTGTGACCTATGCAGAGCTGGACGAGTGCCTGGAGGACCCTGAAACGGGTG AAGTCATAAAGTGGTACGTGTTCCTGATTTCCTTTTGGGGAGAGCAGATTGGCCACAAGGTTAAGAAGAT ATGTGACTGTTACCACTGCCACATCTACCCGTATCCAAACACGGCTGAGGAGCGCAGGGAGATTCAGGAG GGGCTCAATACTCGGATCCAAGACCTTTACACGGTGCTGCACAAAACGGAGGACTATCTGCGGCAAGTGC TCTGCAAAGCTGCCGAGTCCGTGTGCAGCCGTGTGGTCCAGGTGAGGAAGATGAAGGCCATCTACCACAT GCTCAACATGTGCAGCTTTGACGTCACCAACAAGTGCCTCATAGCTGAGGTCTGGTGCCCCGAGGTGGAC CTGCCAGGCTTGCGCAGAGCACTGGAGGAAGGCTCGAGAGAGAGCGGAGCTACAATCCCCTCGTTCATGA ACACAATCCCTACGAAAGAAACACCCCCCACTCTGATCCGCACCAACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3963960), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15420. Forward Primer - name:015420_F_IRAV20-23_A13_Atp6v0a2, sequence:GCAGAAGCTGGAGGTGGA; Reverse Primer - name:015420_R_SP6_IRAV20-23_A13_Atp6v0a2, sequence:TTGTTGGTGCGGATCAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18181 same embryo
	EMAGE:18185 same embryo
	EMAGE:18184 same embryo
	EMAGE:18182 same embryo
	EMAGE:18180 same embryo
	EurExpress:euxassay_011914 same experiment
	MGI:4823318 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source