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EMAGE:18242

Gprin1 G protein-regulated inducer of neurite outgrowth 1 ( MGI:1349455)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242
"Pseudo-wholemount" of euxassay_012022. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012022_01 euxassay_012022_02 euxassay_012022_03 euxassay_012022_04
EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242
euxassay_012022_05 euxassay_012022_06 euxassay_012022_07 euxassay_012022_08 euxassay_012022_09
EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242
euxassay_012022_10 euxassay_012022_11 euxassay_012022_12 euxassay_012022_13 euxassay_012022_14
EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242
euxassay_012022_15 euxassay_012022_16 euxassay_012022_17 euxassay_012022_18 euxassay_012022_19
EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242 EMAGE:18242
euxassay_012022_20 euxassay_012022_21 euxassay_012022_22 euxassay_012022_23 euxassay_012022_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18242Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18242_wholemount_strong.wlz
18242_wholemount_moderate.wlz
18242_wholemount_weak.wlz
18242_wholemount_possible.wlz
18242_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18242_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 15 16 moderate expression: see section 09 10 11 12 17 18 19 20 21 22 23
radius
strong strong
regionalstrong expression: see section 01 02 03 04
ulna
strong strong
regionalstrong expression: see section 01 02
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 21 22 moderate expression: see section 20
hip
moderate moderate
regionalmoderate expression: see section 15
fibula
strong strong
regionalstrong expression: see section 03 04 moderate expression: see section 02
tibia
strong strong
regionalstrong expression: see section 03 04 moderate expression: see section 02 05
femur
strong strong
regionalstrong expression: see section 03 04 20 21 moderate expression: see section 02 05 06 18 19 weak expression: see section 01
meckel's cartilage
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 18 19 20 21 moderate expression: see section 15 16 17
axial skeleton
strong strong
regionalstrong expression: see section 05 06 07 08 13 14 moderate expression: see section 09 10 11 12
basioccipital bone
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 10 13 14
exoccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 16 17
temporal bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 19 20 21
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 06 07 08 10 11 19 20 21
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30692
Entity Detected:Gprin1, G protein-regulated inducer of neurite outgrowth 1 ( MGI:1349455)
Sequence:sense strand is shown

>T30692
GAGCGGACCCTGTTTCCTTAAGAAATTCAGAACTTGTGTCCCCAGTGAAACCAGAACGCTTGTCCTCTGG
GCAGGCAGAACGCGTGTCCTTGGTAAAAACAGAAACATTATCCTCAGGAAAAGAAGATCCAAGGTCTTCT
AGAAGGGTGGATCACACGACTGTCACAGGAAACATGCAAACATCTCAAAAGGGGAATCCCGAGTCTTCAG
GAAAGACAGACCTCGGGTCTTCGAGTTCAGGAGATACCAGGTCTCTGGGGACTTGGGGGTCCCTGTCTGC
TGCAAAAGCTGAGGTGACTGAGGGGAAAGGAGACCCACAGCCCTGGAAGAAGGCAAGTCTCCCAGCCTCA
GAGAAGACCGATCCCCTGGCTTCAAGCAAGGCAGGCTCTGCAAGCCAGGGAAAGGCAGAAACGGTTTCCC
CTGGAGAAGTGGACGCCATGACTTTAGGAAAAACGGTCCCAACGAGTTCAGGAAAAACAGCTTTGGTATC
CCCAGGGAAGGTGGATCTCATGACCTCAGAAAGAGCAGAAGGCATCCCAGAGCTGCAGGCTTCAGAGAAA
GGGAATCCTGTTAACTCCACACGGGTGGACACCGGGGCCACAGGGAGTACTGAGCCCAAATCTGGGGTCA
AAGTGATAACCCAGATTCCAGGTGCCACATCCCCGGGAAAGGTGGAAACTCCGTCTTTGCAAAAGGAGCA
GCCACAACTGTCTGAGAAGACAGATCCCTCCAGAAAAGTCGACCCCCCCACGACTGTGGAGCCTGTGTCC
CTGGGGAAGGCTGACTCTGCATCTCCATCTCCTAGAAAAGCAGAGTCTCAGACTTCCGCCAAGACTGTCC
CTCAGGCCCCAGATAAGGCCACGTCCTCTCTCAGGCAATCAGATGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6825304), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59849. Forward Primer - name:059849_F_IRAV118_c11_Gprin1, sequence:GAGCGGACCCTGTTTCCT; Reverse Primer - name:059849_R_SP6_IRAV118_c11_Gprin1, sequence:TGCCATCTGATTGCCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18244 same embryo
 EMAGE:18243 same embryo
 EurExpress:euxassay_012022 same experiment
 MGI:4825201 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS