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EMAGE:18270

Pdx1 pancreatic and duodenal homeobox 1 ( MGI:102851)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270
"Pseudo-wholemount" of euxassay_012053. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012053_01 euxassay_012053_02 euxassay_012053_03 euxassay_012053_04
EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270
euxassay_012053_05 euxassay_012053_06 euxassay_012053_07 euxassay_012053_08 euxassay_012053_09
EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270
euxassay_012053_10 euxassay_012053_11 euxassay_012053_12 euxassay_012053_13 euxassay_012053_14
EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270
euxassay_012053_15 euxassay_012053_16 euxassay_012053_17 euxassay_012053_18 euxassay_012053_19
EMAGE:18270 EMAGE:18270 EMAGE:18270 EMAGE:18270
euxassay_012053_20 euxassay_012053_21 euxassay_012053_22 euxassay_012053_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18270Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18270_wholemount_strong.wlz
18270_wholemount_moderate.wlz
18270_wholemount_weak.wlz
18270_wholemount_possible.wlz
18270_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18270_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 06 15 17 18 19 20 21 weak expression: see section 04 05 07 08 13 14 16
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 18 19 20 21 22 23
fibula
moderate moderate
regionalmoderate expression: see section 23
tibia
moderate moderate
regionalmoderate expression: see section 23
femur
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 17 18 19 20 21 weak expression: see section 07
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 13 14 15 16 18 19 20 weak expression: see section 10 11
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 12 13 14 weak expression: see section 07 08 09 10 11
basioccipital bone
moderate moderate
regionalmoderate expression: see section 08 10 11 12 13 weak expression: see section 09
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 10 11 12
exoccipital bone
moderate moderate
regionalmoderate expression: see section 03 04 15 16 17
temporal bone
moderate moderate
regionalmoderate expression: see section 02 19 20
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 07 08 17 18 19 weak expression: see section 21 22
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 01 02 18 19 weak expression: see section 03
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 09 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35469
Entity Detected:Pdx1, pancreatic and duodenal homeobox 1 ( MGI:102851)
Sequence:sense strand is shown

>T35469
AAGGGAACTTAACCTAGGCGTCGCACAAGAAGAAAATTCTTGAGGGCACGAGAGCCAGTTGGGTATAGCC
GGAGAGATGCTGGCAGACTTCTGGAAAAACAGCCCTGAGCTTCTGAAAACTTTGAGGCTGCTTCTGATGC
CAAGCGAATGGCCAGATCTGCCTCTAGGACTCTTTCCTGGGACCAATTTAGACAACCTGGGCTCCAAACT
GAGGACAATAAAAAGGGTACAAACTTGAGCGTTCCAATACGGACCAGCAGGCGAGCTGGACCGGGTGCCT
TTGTCTGCCCAGAAACAGAACGGCTGAGCAAGCTAAGGTTAAGAATAACGAAAGGCGGCTGTTGGAGGTT
TCAAAGAGAGCGCTTCTTCCAATCCCCCCTCCTCTTCCCCCTCCCTCACCTCCTTTTCCTTATTTCCCTT
TCCTAGAGTCGTTCCCCCTCTTCAGCCAAATATTTCATTAATCTCTATCCTTCAACCTATACCATTTCCT
TTGAAAATCAGAAATTCTTCACACCCGGACCTCCTCGTGCCCCTAATGGGACCTCCAGGGTCCTGAGGGA
CAAAGATGCAGAAGGACATTTGAAGTTTCTGTTTTAGAGCGCGAATATCCTCCTGAAAGGAAGGCAGGTG
CCCTGGTACACCTTGGAAAACTAGTCACAACGCCCTGAATTAGAAGATGGGAGAGAGGAGCAGGGCAAAA
GCTGGTTGTTTTCATTTAAAGCATTAAATTAACCATTCTTTGCCAACAGGTCTACAATTTGAAGTCAGTC
AGTTGCTCCTTTCAAGGTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94684. Forward Primer - name:094684_F_cDNA_Ipf1, sequence:AAGGGAACTTAACCTAGGCGTC; Reverse Primer - name:094684_N_SP6_cDNA_Ipf1, sequence:AGACCTTGAAAGGAGCAACTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18272 same embryo
 EMAGE:18271 same embryo
 EurExpress:euxassay_012053 same experiment
 MGI:4827139 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS