Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18271

Cd59a CD59a antigen ( MGI:109177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271
"Pseudo-wholemount" of euxassay_012059. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012059_01 euxassay_012059_02 euxassay_012059_03 euxassay_012059_04
EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271
euxassay_012059_05 euxassay_012059_06 euxassay_012059_07 euxassay_012059_08 euxassay_012059_09
EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271
euxassay_012059_10 euxassay_012059_11 euxassay_012059_12 euxassay_012059_13 euxassay_012059_14
EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271
euxassay_012059_15 euxassay_012059_16 euxassay_012059_17 euxassay_012059_18 euxassay_012059_19
EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271 EMAGE:18271
euxassay_012059_20 euxassay_012059_21 euxassay_012059_22 euxassay_012059_23 euxassay_012059_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18271Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18271_wholemount_strong.wlz
18271_wholemount_moderate.wlz
18271_wholemount_weak.wlz
18271_wholemount_possible.wlz
18271_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18271_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 20 21
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 weak expression: see section 20 21 22 23
fibula
weak weak
regionalweak expression: see section 24
tibia
weak weak
regionalweak expression: see section 24
femur
moderate moderate
regionalmoderate expression: see section 05 weak expression: see section 01 02 03 04 21 22
brain
moderate moderate
homogeneousmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 02 03
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 18 weak expression: see section 04 05
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 05 06
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 16 17 18 19 weak expression: see section 04 05 06 07 08
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 07
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 07
spinal cord
moderate moderate
homogeneousmoderate expression: see section 06 07 08 09 10 11 12 13 14
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 14 15 weak expression: see section 05 06 07 08
neural retina
moderate moderate
regionalmoderate expression: see section 22 23 24 weak expression: see section 02 03 04
meckel's cartilage
weak weak
regionalweak expression: see section 04 05 06 08 16 17 20
axial skeleton
weak weak
regionalweak expression: see section 06 07 08
basioccipital bone
weak weak
regionalweak expression: see section 08 10 13
basisphenoid bone
weak weak
regionalweak expression: see section 10 13
exoccipital bone
weak weak
regionalweak expression: see section 01 02 03
temporal bone
weak weak
regionalweak expression: see section 01 02 03 20 21
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 03 06 07 08 19 20
viscerocranium
weak weak
regionalExpression in the turbinate bone.
scapula
weak weak
regionalweak expression: see section 01 02 20
pelvic girdle skeleton
weak weak
regionalweak expression: see section 09 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36134
Entity Detected:Cd59a, CD59a antigen ( MGI:109177)
Sequence:sense strand is shown

>T36134
GTCTCTATGCTGTAGCCGGAATGCAAGTGTATCAAAGGTGTTGGAAACAATCAGATTGTCATGGTGAGAT
CATTATGGACCAATTAGAAGAGACAAAATTAAAATTCAGATGCTGCCAGTTTAACTTGTGTAACAAAAGT
GACGGATCCTTGGGGAAGACACCATTGCTGGGGACCTCGGTTCTGGTGGCCATTTTGAATCTTTGTTTCT
TAAGTCATCTCTAAGTCCTAGATGCCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTCCTGCTGCT
GCAGTAGTCTAAAGCTTTTATTATTTTCCATCTGTATTAATTTAGGAAAGAATAAAGTTCACTTGAACAT
CTTGGACAAGAGGGAGGAGTTTAAGCAGTGTGGAAGAGCACCATGCACACCAGGAGAGCCCACTTGCAGT
TGCAGAGTGAAGGCGGAGGGTAGAACACCTATAGGTTATATAATCTTCCTTTGCCTGACTTTAAGATTCA
GGTTAAGTGAGTTCCTGGCAACCCTCAGGGATAGCTCTCTGGTTCCTGCAATGTAGCTGAGGAGTGAGAT
CAGACCTTTAGGAGTGAGTGGGCCAGGAGGATTCTCTTTACCACTCTGACACGCTCAAAAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95149. Forward Primer - name:095149_F_cDNA_Cd59a, sequence:GTCTCTATGCTGTAGCCGGAAT; Reverse Primer - name:095149_N_SP6_cDNA_Cd59a, sequence:ATTTTGAGCGTGTCAGAGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18272 same embryo
 EMAGE:18270 same embryo
 EurExpress:euxassay_012059 same experiment
 MGI:4823738 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS