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EMAGE:18274

Ugt1a6a UDP glucuronosyltransferase 1 family, polypeptide A6A ( MGI:2137698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274
"Pseudo-wholemount" of euxassay_012067. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012067_01 euxassay_012067_02 euxassay_012067_03 euxassay_012067_04
EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274
euxassay_012067_05 euxassay_012067_06 euxassay_012067_07 euxassay_012067_08 euxassay_012067_09
EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274
euxassay_012067_10 euxassay_012067_11 euxassay_012067_12 euxassay_012067_13 euxassay_012067_14
EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274 EMAGE:18274
euxassay_012067_15 euxassay_012067_16 euxassay_012067_17 euxassay_012067_18 euxassay_012067_19
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euxassay_012067_20 euxassay_012067_21 euxassay_012067_22 euxassay_012067_23 euxassay_012067_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18274Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18274_wholemount_strong.wlz
18274_wholemount_moderate.wlz
18274_wholemount_weak.wlz
18274_wholemount_possible.wlz
18274_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18274_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 18 19 moderate expression: see section 17
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 18 19 20 21 22
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 weak expression: see section 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37192
Entity Detected:Ugt1a6a, UDP glucuronosyltransferase 1 family, polypeptide A6A ( MGI:2137698)
Sequence:sense strand is shown

>T37192
TCCCTACCTTCCTTACACCAGAATTCTCTGTGGCTGTTACGGTACGATTTTGTGTTCGAATATCCCAGGC
CGGTCATGCCCAACATGATCTTCCTAGGAGGGATCAACTGTAAGAAGAAGGGAAAGCTGACTCAGGAATT
TGAAGCCTATGTCAACGCCTCTGGGGAGCATGGCATCGTTGTTTTCTCTTTGGGATCCATGGTCTCAGAG
ATTCCGGAGAAGAAAGCCATGGAAATTGCTGAGGCTTTGGGCAGAATTCCTCAGACGGTCCTGTGGCGCT
ACACCGGAACTAGACCATCGAATCTTGCAAAGAACACAATTCTTGTCAAATGGCTACCCCAAAATGATCT
GCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCTGGCTCCCATGGTATTTATGAAGGAATATGC
AATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACAATGCCAAGCGCATGGAAACTC
GGGGAGCTGGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTTGGAAAATGCCCTTAAAACTGT
CATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTCACAAGGACCGTCCTATAGAG
CCTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101929. Forward Primer - name:101929_F_cDNA_Ugt1a6, sequence:TCCCTACCTTCCTTACACCAGA; Reverse Primer - name:101929_N_SP6_cDNA_Ugt1a6, sequence:CCTTGTGCCTCATCACGTATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18273 same embryo
 EurExpress:euxassay_012067 same experiment
 MGI:4829083 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS