EMAGE mouse gene expression spatial database

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EMAGE:18285

Svop SV2 related protein ( MGI:1915916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012084. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012084_01	euxassay_012084_02	euxassay_012084_03	euxassay_012084_04

euxassay_012084_05	euxassay_012084_06	euxassay_012084_07	euxassay_012084_08	euxassay_012084_09

euxassay_012084_10	euxassay_012084_11	euxassay_012084_12	euxassay_012084_13	euxassay_012084_14

euxassay_012084_15	euxassay_012084_16	euxassay_012084_17	euxassay_012084_18	euxassay_012084_19

euxassay_012084_20	euxassay_012084_21	euxassay_012084_22	euxassay_012084_23	euxassay_012084_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18285_wholemount_strong.wlz

18285_wholemount_moderate.wlz

18285_wholemount_weak.wlz

18285_wholemount_possible.wlz

18285_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18285_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 03 04 05 06 07 18 19 20 21 22 23

femur

weak

regional

weak expression: see section 05 19 20 21

forebrain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02

hindbrain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 06 07 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21 22 weak expression: see section 09

vagus x ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 17 18 19

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 12 17 weak expression: see section 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

pelvic girdle skeleton

weak

regional

weak expression: see section 07 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30708
Entity Detected:	Svop, SV2 related protein ( MGI:1915916)
Sequence:	sense strand is shown >T30708 GCTGCTCACTTCGGTGGTCTTCATCGGCATGATGTCCAGTTCCACGCTCTGGGGAAACATCTCGGACCAG TATGGCAGGAAAACTGGGCTGAAGATCAGTGTGCTCTGGACCCTGTACTATGGCATCCTCAGCGCCTTCG CGCCCGTGTATAGCTGGATCCTGGTGCTCCGAGGCCTGGTGGGCTTTGGGATTGGAGGGGTGCCCCAGTC GGTGACGCTGTACGCCGAGTTCCTTCCCATGAAGGCCAGAGCCAAGTGCATTTTGCTGATTGAGGTCTTC TGGGCCATAGGGACCGTGTTCGAGGTGCTTCTGGCTGTGTTTGTGATGCCCAGCCTGGGCTGGCGCTGGC TGCTCCTGCTCTCGGCCGCTCCACTACTACTCTTCGCTGTTCTGTGCTTCTGGCTGCCAGAGAGTGCTAG GTACGATGTGCTGTCCGGGAACCAGGAAAAGGCAATCGCTACTTTAAAGCGGATTGCAACGGAAAATGGA GCCCCCATGCCTCTGGGAAAGCTTATCATCTCCAGACAGGAAGACCGAGGCAAAATGAGGGACCTTTTCA CACCCCACTTTCGTTGGACAACTCTGCTGCTGTGGTTTATATGGTTCTCCAATGCCTTCTCTTACTACGG CTTGGTTCTGCTCACCACGGAACTCTTCCAGGCAGGAGACGTCTGCAGCATCTCCAGCCGGAAGAAGGCA GTGGAAGCCAAATGCAGCTTGGCCTGTGAGTACCTGAGCAAAGAGGATTACATGGACCTGCTGTGGACCA CCCTGTCCGAGTTCCCAGGTGTCCTTGTGACTCTGTGGGTCATCGACCGCCTGGGCCGCAAGAAGACCAT GGCTCTGTGTTTCATCATCTTCTCCCTCTGCAGCCTCCTGCTGTTCATCTGCATTGGAAGAAATGTGCTA ACCCTCTTACTGTTCATCGCCAGAGCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6812082), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60394. Forward Primer - name:060394_F_IRAV119_c11_1110030H18Rik, sequence:GCTGCTCACTTCGGTGGT; Reverse Primer - name:060394_R_SP6_IRAV119_c11_1110030H18Rik, sequence:AACGCTCTGGCGATGAAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18287 same embryo
	EMAGE:18288 same embryo
	EMAGE:18290 same embryo
	EMAGE:18289 same embryo
	EMAGE:18286 same embryo
	EurExpress:euxassay_012084 same experiment
	MGI:4828549 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source