EMAGE mouse gene expression spatial database

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EMAGE:18290

Metrn meteorin, glial cell differentiation regulator ( MGI:1917333)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012079. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012079_01	euxassay_012079_02	euxassay_012079_03	euxassay_012079_04

euxassay_012079_05	euxassay_012079_06	euxassay_012079_07	euxassay_012079_08	euxassay_012079_09

euxassay_012079_10	euxassay_012079_11	euxassay_012079_12	euxassay_012079_13	euxassay_012079_14

euxassay_012079_15	euxassay_012079_16	euxassay_012079_17	euxassay_012079_18	euxassay_012079_19

euxassay_012079_20	euxassay_012079_21	euxassay_012079_22	euxassay_012079_23	euxassay_012079_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18290_wholemount_strong.wlz

18290_wholemount_moderate.wlz

18290_wholemount_weak.wlz

18290_wholemount_possible.wlz

18290_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18290_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 moderate expression: see section 09 13 14 15

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 moderate expression: see section 09 14 15

telencephalon ventricular layer

strong

regional

strong expression: see section 10 moderate expression: see section 03 04 08 09 11 12 13 14 15 16 19 22 23 weak expression: see section 05 06 07 17 18 20 21

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 19 20 weak expression: see section 12 13 14 15 16 17 18 21 22 23

pons ventricular layer

moderate

regional

moderate expression: see section 09 10 11 19 weak expression: see section 12 13 14 15 16 17 18

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

spinal cord ventricular layer

strong

regional

strong expression: see section 10 moderate expression: see section 11 13

neural retina

moderate

regional

moderate expression: see section 01 02 03 21 22 23 24

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30707
Entity Detected:	Metrn, meteorin, glial cell differentiation regulator ( MGI:1917333)
Sequence:	sense strand is shown >T30707 GGATGCTGGGAGAAGGGTTTAGTCTCTTGCTCCTGTACCTTTTCCTCTTGGGAGGAGCTGTGGGCTCGTG CTGAGAGATCACAGGCCTGGCTGATGACCTGCCTTGCATGCTAGGTGCCTGCAGGCCCTGCAGTGATGCC GAGCTCCTCCTGGCTGCATGCACCAGTGATTTTGTGATCCACGGGACCATCCATGGGGTCGCCCATGACA CAGAGCTGCAAGAATCAGTCATCACTGTGGTGGTTGCTCGTGTCATCCGCCAGACACTGCCACTGTTCAA GGAAGGGAGCTCGGAGGGCCAAGGCCGGGCCTCCATTCGTACCTTGCTGCGCTGTGGTGTGCGTCCTGGC CCAGGCTCCTTCCTCTTCATGGGCTGGAGCCGATTTGGCGAAGCTTGGCTGGGCTGTGCTCCCCGCTTCC AAGAGTTCAGTCGTGTCTATTCAGCTGCTCTCACGACCCATCTCAACCCATGTGAGATGGCACTGGACTG AGAGACCTGGGAGCAAGCCCTGGATGGACCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6819134), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63059. Forward Primer - name:063059_F_IRAV119_c04_Metrn, sequence:GGATGCTGGGAGAAGGGT; Reverse Primer - name:063059_R_SP6_IRAV119_c04_Metrn, sequence:GAAGGTCCATCCAGGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18287 same embryo
	EMAGE:18288 same embryo
	EMAGE:18289 same embryo
	EMAGE:18286 same embryo
	EMAGE:18285 same embryo
	EurExpress:euxassay_012079 same experiment
	MGI:4826166 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source