EMAGE mouse gene expression spatial database

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EMAGE:18317

Acot6 acyl-CoA thioesterase 6 ( MGI:1921287)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012106. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012106_01	euxassay_012106_02	euxassay_012106_03	euxassay_012106_04

euxassay_012106_05	euxassay_012106_06	euxassay_012106_07	euxassay_012106_08	euxassay_012106_09

euxassay_012106_10	euxassay_012106_11	euxassay_012106_12	euxassay_012106_13	euxassay_012106_14

euxassay_012106_15	euxassay_012106_16	euxassay_012106_17	euxassay_012106_18	euxassay_012106_19

euxassay_012106_20	euxassay_012106_21	euxassay_012106_22	euxassay_012106_23	euxassay_012106_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18317_wholemount_strong.wlz

18317_wholemount_moderate.wlz

18317_wholemount_weak.wlz

18317_wholemount_possible.wlz

18317_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18317_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 05 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04 06 07 08

humerus

moderate

regional

moderate expression: see section 01 21 22 23 24 weak expression: see section 02

forelimb digit 2 phalanx

moderate

regional

moderate expression: see section 01

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 01

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 07

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 07 08 09

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 07

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 07 08 09

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 07

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 07 08 09

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 07

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 07 08 09

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 09

tarsus

moderate

regional

moderate expression: see section 06 07

fibula

moderate

regional

moderate expression: see section 01 02 04 05

tibia

moderate

regional

moderate expression: see section 01 02 04 05 weak expression: see section 03

femur

moderate

regional

moderate expression: see section 01 02 05 06 08 09 20 21 22 23 24

otic capsule

moderate

regional

moderate expression: see section 05 06 07 08 16 17

naris

moderate

regional

moderate expression: see section 13 14 15 16 17 18

nasal septum

moderate

regional

moderate expression: see section 15

viscerocranium

moderate

regional

Expression in the turbinate bone.

hyoid

moderate

regional

moderate expression: see section 10 11 12 13 14 15 weak expression: see section 08 09

meckel's cartilage

strong

regional

strong expression: see section 05 16 17 18 20 21 22 moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 19 23

arytenoid cartilage

moderate

regional

moderate expression: see section 12

cricoid cartilage

moderate

regional

moderate expression: see section 12

thyroid cartilage

moderate

regional

moderate expression: see section 09 12 13 14 weak expression: see section 10 11

axial skeleton

moderate

regional

moderate expression: see section 05 09 10 11 12 13 14 15 16 17 18 weak expression: see section 06 07 08

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 04 05 06 07 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

moderate

homogeneous

moderate expression: see section 02 03 05 06 07 08 09 10 11 12 13 14 15 16

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 18 19 20 21 22 23 24

vault of skull

moderate

homogeneous

moderate expression: see section 01

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 05 06 07 08 19 20 21 22 23 24

clavicle

moderate

regional

moderate expression: see section 16 17 18 19 20 weak expression: see section 04 05 06

scapula

moderate

regional

moderate expression: see section 01 21 22

sternum

moderate

regional

moderate expression: see section 12

pelvic girdle skeleton

moderate

regional

moderate expression: see section 10 11 12 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30740
Entity Detected:	Acot6, acyl-CoA thioesterase 6 ( MGI:1921287)
Sequence:	sense strand is shown >T30740 TCTCCACCCACCTACCCACCCGGTTCCTAAGCTCACAGCCTAGTCTATTTTTTGGTCCTGAATAGACTCC ATGTAGGGTCTGTCACATAACTGTAACTTTAGTAACCTTGTCCCCACTCTCCAAATTCTCTCAATCTTCT TTAAGAGTAAATTTTCTACCAAGTTTCCTTCCCTTTAAGACACCATGAATTCCTCAACTATATGCTGAAG AGGGAGTGCAACTTGAAATCCACTAAATGCCAGCCACACGAGGGAGGGAGTACTGATAACTTATGCCTGT AGTAAGAGTGACTTTTGCCTCCTTTCACATCAAACTTTCAAAGAGCCTTTAACCCACAAATGAAGACAAT GTTCCCTAACCATTAAAGCGCACAAAAATGTTTACTACAAAACCTTTCCCTACCATAAACATGCTGGTGG TGGTTTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3485583), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13644. Forward Primer - name:013644_F_IRAV01-03_F23_4632408A20Rik, sequence:TCTCCACCCACCTACCCA; Reverse Primer - name:013644_R_SP6_IRAV01-03_F23_4632408A20Rik, sequence:TGAAAACCACCACCAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18316 same embryo
	EMAGE:18315 same embryo
	EMAGE:18314 same embryo
	EMAGE:18313 same embryo
	EMAGE:18312 same embryo
	EurExpress:euxassay_012106 same experiment
	MGI:4822927 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source