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EMAGE:18328

Zfp553 zinc finger protein 553 ( MGI:2384725)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328
euxassay_012141_01 euxassay_012141_02 euxassay_012141_03 euxassay_012141_04 euxassay_012141_05
EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328
euxassay_012141_06 euxassay_012141_07 euxassay_012141_08 euxassay_012141_09 euxassay_012141_10
EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328
euxassay_012141_11 euxassay_012141_12 euxassay_012141_13 euxassay_012141_14 euxassay_012141_15
EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328 EMAGE:18328
euxassay_012141_16 euxassay_012141_17 euxassay_012141_18 euxassay_012141_19 euxassay_012141_20
EMAGE:18328 EMAGE:18328 EMAGE:18328
euxassay_012141_21 euxassay_012141_22 euxassay_012141_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18328Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18328_wholemount_strong.wlz
18328_wholemount_moderate.wlz
18328_wholemount_weak.wlz
18328_wholemount_possible.wlz
18328_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18328_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22
radius
strong strong
regionalstrong expression: see section 01
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 19 20 21 22 23
femur
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23
otic capsule
strong strong
regionalstrong expression: see section 09 15
naris
strong strong
regionalstrong expression: see section 09 10 13 14 15 16
nasal septum
strong strong
regionalstrong expression: see section 12
viscerocranium
strong strong
regionalExpression in the turbinate bone.
hyoid
strong strong
regionalstrong expression: see section 10 11 12 13 14
meckel's cartilage
strong strong
regionalstrong expression: see section 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16
basioccipital bone
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
basisphenoid bone
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 17 18 19 20 21 22
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 06 07 08 17 18 19 20 21 22
clavicle
strong strong
regionalstrong expression: see section 08 09 15 16 17 18
scapula
strong strong
regionalstrong expression: see section 03 04 05 19 20 21
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 10 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31459
Entity Detected:Zfp553, zinc finger protein 553 ( MGI:2384725)
Sequence:sense strand is shown

>T31459
CAGTGATGGGCCTTTTGGCCTACCTGGCTTGGAACCAGAGCCTGGGGGCCCGCAGGCTGGGGAGCCGCCC
CCACCTCTGGCAGGTGACAAGCCCCACAAGTGTCCTGAGTGTGGCAAAGGTTTCCGACGAAGCTCTGATC
TGGTGAAACACCATCGTGTGCACACAGGGGAGAAACCCTACCTCTGCCCTGAGTGTGGCAAAGGCTTTGC
TGACAGTTCTGCCCGAGTCAAACACCTCCGCACCCACCAGGGCGAACGCACTAGACCACCACCACCACCA
TCTACTCTCCTGCGGCCCCATAATCCACCTGGTTCTGTACCCATTGTCCCTCAGTCTCGAGTGCAGGGTC
GGCCCTCCGGACCCAGCCAGCTTCACGTGTGTGGCTTCTGTGGGAAAGAGTTCCCCCGGAGCTCAGATTT
AGTCAAACACAGGCGTACACATACCGGGGAGAAGCCATACAAATGTGCAGAATGTGGTAAGGGCTTTGGT
GACAGCTCTGCTCGAATAAAGCACCAGCGTGGGCATCTGGCCTTAAAGCCCTTTGGGGTGGGGGATGGTC
CGCCAAGGCCCCTTAAGGAGGAGTCACCAGCTGGACTGGAGTGATGGGAATTCTAGGGAGGGTAGAAGCC
CAGGGTACCGGAGGGAGGGTGTCTGCAGGTGAAGGCAGAGAAGAGCCTGGGAAACGGTGGGAGGGAAGAG
GAGGATGAGAATAGCTAGAAACAGATTAGAGACTACATAACCTTCATGCTCAGGAAACTGTCTTGGTTAG
GCGCTGGGACCTTGCCAGTGTGAGCTGTATCGCAGCTTCTAGAACACTGCCTAGCACAGATTAGGCCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4209324), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26463. Forward Primer - name:026463_F_IRAV59-62_L05_Zfp553, sequence:CAGTGATGGGCCTTTTGG; Reverse Primer - name:026463_R_SP6_IRAV59-62_L05_Zfp553, sequence:GGGGGCCTAATCTGTGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18326 same embryo
 EMAGE:18330 same embryo
 EMAGE:18327 same embryo
 EMAGE:18329 same embryo
 EMAGE:18325 same embryo
 EurExpress:euxassay_012141 same experiment
 MGI:4829319 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS