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EMAGE:18344

Mospd2 motile sperm domain containing 2 ( MGI:1924013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344
"Pseudo-wholemount" of euxassay_014284. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014284_01 euxassay_014284_02 euxassay_014284_03 euxassay_014284_04
EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344
euxassay_014284_05 euxassay_014284_06 euxassay_014284_07 euxassay_014284_08 euxassay_014284_09
EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344
euxassay_014284_10 euxassay_014284_11 euxassay_014284_12 euxassay_014284_13 euxassay_014284_14
EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344 EMAGE:18344
euxassay_014284_15 euxassay_014284_16 euxassay_014284_17 euxassay_014284_18 euxassay_014284_19
EMAGE:18344 EMAGE:18344 EMAGE:18344
euxassay_014284_20 euxassay_014284_21 euxassay_014284_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18344Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18344_wholemount_strong.wlz
18344_wholemount_moderate.wlz
18344_wholemount_weak.wlz
18344_wholemount_possible.wlz
18344_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18344_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 15 16 17 19 20 weak expression: see section 18 21
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 19 20 21 22 weak expression: see section 05 18
fibula
strong strong
regionalstrong expression: see section 02
tibia
strong strong
regionalstrong expression: see section 01 02
femur
strong strong
regionalstrong expression: see section 05 06 07 08 19 20 21 22 moderate expression: see section 04 09 18
meckel's cartilage
strong strong
regionalstrong expression: see section 05 06 07 08 09 14 15 16 moderate expression: see section 03 04 10 11 12 13
axial skeleton
strong strong
regionalstrong expression: see section 12 13 moderate expression: see section 08 09 10 11 14 15
basioccipital bone
strong strong
regionalstrong expression: see section 10 11 12 13 moderate expression: see section 09 14
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 09 14
exoccipital bone
strong strong
regionalstrong expression: see section 06 moderate expression: see section 03 04 05 17 weak expression: see section 18 19
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 19 20 21
vault of skull
moderate moderate
regionalmoderate expression: see section 01 02 04 weak expression: see section 19 20
orbito-sphenoid
strong strong
regionalstrong expression: see section 05 06 moderate expression: see section 01 02 03 04 15 16 17
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
weak weak
regionalweak expression: see section 06 09
scapula
strong strong
regionalstrong expression: see section 03 04 19 weak expression: see section 18 20
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 11 moderate expression: see section 16 weak expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31452
Entity Detected:Mospd2, motile sperm domain containing 2 ( MGI:1924013)
Sequence:sense strand is shown

>T31452
TGGGTGGGACAGATCCATTCAAGTATAGCTACCCACCACTGGTAGATGATGACTTCCAGACACCACTTTG
TGAAAATGGGCCAATTGCCAGTGAGGATGAAACCTCAAGTAAGGAAGATATAGAAGGTGATGGTAAGGAA
ACCTTGGAAACGATTTCTAATGAGGAGCCATCAGCTCTGTCTGAAAAGAGTAACCCAACTGAGTCTGTTT
CCAAAAAAGATGAAAATGAAAAAGTTGATTCAAAGACGAAAACTTTTAAAAAGCCATTGAGTGTTTTTAA
AGGACCCTTATTACACATCAGCCCAGCAGAGGAATTATATTTTGGAAGCATTGAATCTGGAGAGAAGAAA
ACTCTGATAGTGTTGACAAATGTCACAAAAAATATTGTGGCCTTTAAGGTGAGAACCACAGCTCCAGAAA
AATACAGGGTCAAGCCTAGCAACAGCAGCTGCGATCCCGGAGCTTCCATTGATATCATCGTTTCTCCACA
CGGAGGTTTAACCGTCTCAGCTCAAGACCGGTTCCTGATCATGGCTGCCGAAATGGAGCAGTCATCTGGC
ACTGGGCCTGCAGAACTGAGTCAGTTCTGGAAAGAGGTTCCAAGAAACAAGGTGATGGAGCACAGGCTAA
GATGTCACACCGTTGAGAGCAGCAAACCGAACAGTCTCATGCTGAAGGACAGCATTTCCACCATGTCAGA
TAAAACCAGTGAAGATCTGTACCTGCAATTTGCCACCTCCAGATGTGAAACAGACTGCAGTCCACACTAA
GTACTATGTCCCTGTATGTTCACAGTGACTTCCCAAGTGGTAGAGTTGGGGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4223175), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26523. Forward Primer - name:026523_F_IRAV59-62_J19_Mospd2, sequence:TGGGTGGGACAGATCCAT; Reverse Primer - name:026523_R_SP6_IRAV59-62_J19_Mospd2, sequence:GGCCCCCAACTCTACCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18346 same embryo
 EMAGE:18345 same embryo
 EMAGE:18347 same embryo
 EMAGE:18343 same embryo
 EurExpress:euxassay_014284 same experiment
 MGI:4826424 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS