EMAGE mouse gene expression spatial database

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EMAGE:18406

Cit citron ( MGI:105313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014352. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014352_01	euxassay_014352_02	euxassay_014352_03	euxassay_014352_04

euxassay_014352_05	euxassay_014352_06	euxassay_014352_07	euxassay_014352_08	euxassay_014352_09

euxassay_014352_10	euxassay_014352_11	euxassay_014352_12	euxassay_014352_13	euxassay_014352_14

euxassay_014352_15	euxassay_014352_16	euxassay_014352_17	euxassay_014352_18	euxassay_014352_19

euxassay_014352_20	euxassay_014352_21	euxassay_014352_22	euxassay_014352_23	euxassay_014352_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18406_wholemount_strong.wlz

18406_wholemount_moderate.wlz

18406_wholemount_weak.wlz

18406_wholemount_possible.wlz

18406_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18406_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03

radius

moderate

regional

moderate expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 04 05

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 04 05 19 20 21

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 04 05 19 20 21

fibula

moderate

regional

moderate expression: see section 01 02 23 24

tibia

moderate

regional

moderate expression: see section 01 02 22 23 24

femur

moderate

regional

moderate expression: see section 01 02 03 04 05 06 16 17 18 19 20 21 22 23 24

otic capsule

moderate

regional

moderate expression: see section 07 08 09 14 15 16 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

hyoid

moderate

regional

moderate expression: see section 15 16 weak expression: see section 12 13 14

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 16 17 18 19 20 21 22

thyroid cartilage

weak

regional

weak expression: see section 13

axial skeleton

moderate

regional

moderate expression: see section 07 12 13 14 15 16 17 18

axial skeleton cervical region

moderate

regional

moderate expression: see section 08 09 10 11

basioccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 18 19 20 21 22 23 24

vault of skull

moderate

regional

moderate expression: see section 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

clavicle

moderate

regional

moderate expression: see section 06 07 08 09 19 20

scapula

moderate

regional

moderate expression: see section 02 03 04 05 22 23 24

pelvic girdle skeleton

moderate

regional

moderate expression: see section 07 08 09 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31535
Entity Detected:	Cit, citron ( MGI:105313)
Sequence:	sense strand is shown >T31535 ATCGCCATGTCTGCCATTGTGCGGTCGCCCGAGCACCAGCCCAGTGCCATGAGCCTGCTTGCCCCACCAT CCAGCCGCAGAAAGGAGTCGTCAACTCCAGAGGAATTCAGCCGGCGTCTGAAAGAGCGCATGCACCACAA CATCCCTCACCGGTTTAATGTGGGCCTGAACATGAGAGCCACCAAGTGCGCCGTGTGTCTGGATACTGTG CACTTTGGACGCCAGGCATCCAAATGCCTCGAATGTCAAGTGATGTGTCACCCCAAATGCTCCACCTGCT TGCCCGCTACCTGTGGCCTGCCAGCTGAATATGCCACACACTTCACTGAGGCCTTCTGCCGGGACAAAAT GAACTCCCCGGGGCTCCAGAGCAAGGAGCCTGGCAGCAGCTTGCACCTGGAAGGGTGGATGAAAGTGCCC AGAAATAACAAACGGGGACAGCAAGGCTGGGACAGGAAGTACATTGTTCTGGAGGGGTCAAAAGTCCTCA TCTATGACAATGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGAAGAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5346007), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 62794. Forward Primer - name:062794_F_IRAV67-70_G22_Cit, sequence:ATCGCCATGTCTGCCATT; Reverse Primer - name:062794_R_SP6_IRAV67-70_G22_Cit, sequence:AATTCTTCCACCGGCCTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18408 same embryo
	EMAGE:18407 same embryo
	EurExpress:euxassay_014352 same experiment
	MGI:4823883 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source