EMAGE mouse gene expression spatial database

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EMAGE:18420

Myl4 myosin, light polypeptide 4 ( MGI:97267)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014373. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014373_01	euxassay_014373_02	euxassay_014373_03	euxassay_014373_04

euxassay_014373_05	euxassay_014373_06	euxassay_014373_07	euxassay_014373_08	euxassay_014373_09

euxassay_014373_10	euxassay_014373_11	euxassay_014373_12	euxassay_014373_13	euxassay_014373_14

euxassay_014373_15	euxassay_014373_16	euxassay_014373_17	euxassay_014373_18	euxassay_014373_19

euxassay_014373_20	euxassay_014373_21	euxassay_014373_22	euxassay_014373_23	euxassay_014373_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18420_wholemount_strong.wlz

18420_wholemount_moderate.wlz

18420_wholemount_weak.wlz

18420_wholemount_possible.wlz

18420_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18420_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 22 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

hand

strong

regional

strong expression: see section 01 02 03

foot

strong

regional

strong expression: see section 07 08 09 10 19 20 21 22

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22 23 24

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22 23 24

heart atrium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

heart ventricle

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

tail paraxial mesenchyme

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31855
Entity Detected:	Myl4, myosin, light polypeptide 4 ( MGI:97267)
Sequence:	sense strand is shown >T31855 CATCCTAACTGGGCCACGCCCAAAGAGCAACCGACAGTGTCCATATACCTGCCTGGTCCCACCTCCACTG GAGAGCCTAAAGCAACATGCCTCCCAAGAAACCCGAGCCTAAGAAGGAGACTGCCAAGCCGGCTGCAGCC CCTGCTCCAGCTGCATCCGCAGCTCCGGAGCCCCTCAAGGACTCTGCCTTTGACCCAAAGAGTGTGAAGA TAGACTTCAGTGCTGACCAGATCGAAGAATTCAAAGAGGCCTTTTCATTGTTTGACCGGACTCCAACGGG AGAGATGAAGATCACCTACGGGCAGTGTGGGGACGTGCTGCGGGCCCTGGGCCAGAACCCCACCAACGCA GAGGTGCTGCGCGTTTTGGGCAAACCCAAGCCTGAAGAGATGAGTTCCAAGACACTGGACTTCGAGATGT TCCTGCCCATCCTGCAACACATCTCCCGCAACAAGGAGCAGGGCACCTATGAGGACTTCGTGGAGGGGCT GCGGGTCTTTGACAAAGAAAGCAACGGCACAGTCATGGGTGCAGAGCTTCGGCATGTCCTTGCTACCCTG GGAGAGAAGATGAGCGAGGCAGAGGTGGAGCAGCTGTTGTCTGGGCAGGAGGATGCCAATGGCTGCATCA ACTATGAAGCCTTTGTCAAGCACATCATGTCTGGGTAAAGCACGTTTCTCCAGGGTGCTTGGCCCTGCGT GAGAAAGCAAGAGATCCGCAGCGGCTGTTACTGTTACGGAGTATTGGACTCACATGGCTTCTCTGACCCA CAACCTTCCCTGGAAATAAAGAGCCCCAGCTTGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6741975), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60982. Forward Primer - name:060982_F_IRAW2_d02_Myl4, sequence:CATCCTAACTGGGCCACG; Reverse Primer - name:060982_R_SP6_IRAW2_d02_Myl4, sequence:ACTCCAAGCTGGGGCTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18423 same embryo
	EMAGE:18421 same embryo
	EMAGE:18419 same embryo
	EMAGE:18422 same embryo
	EMAGE:18418 same embryo
	EurExpress:euxassay_014373 same experiment
	MGI:4826550 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source