EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18422

Tcam1 testicular cell adhesion molecule 1 ( MGI:1923120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014376. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014376_01	euxassay_014376_02	euxassay_014376_03	euxassay_014376_04

euxassay_014376_05	euxassay_014376_06	euxassay_014376_07	euxassay_014376_08	euxassay_014376_09

euxassay_014376_10	euxassay_014376_11	euxassay_014376_12	euxassay_014376_13	euxassay_014376_14

euxassay_014376_15	euxassay_014376_16	euxassay_014376_17	euxassay_014376_18	euxassay_014376_19

euxassay_014376_20	euxassay_014376_21	euxassay_014376_22	euxassay_014376_23	euxassay_014376_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18422_wholemount_strong.wlz

18422_wholemount_moderate.wlz

18422_wholemount_weak.wlz

18422_wholemount_possible.wlz

18422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 03 04 05 22 23 24

forelimb digit 2 metacarpal

strong

regional

strong expression: see section 02

forelimb digit 2 phalanx

strong

regional

strong expression: see section 02

forelimb digit 3 metacarpal

strong

regional

strong expression: see section 01

forelimb digit 3 phalanx

strong

regional

strong expression: see section 01

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 05 23

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 05 07 08 22 23

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 06 23

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 06 07 08 22 23

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 06

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 06 07 08 22

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 08

fibula

strong

regional

strong expression: see section 01 02 03 04

tibia

strong

regional

strong expression: see section 01 02 03 04

femur

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

otic capsule

strong

regional

strong expression: see section 08 09 10 11 17 18 19 20 21

nasal septum

strong

regional

strong expression: see section 13

viscerocranium

strong

regional

Expression in the turbinate bone.

heart valve

moderate

regional

moderate expression: see section 15 16

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 22 23 24

bladder

moderate

regional

moderate expression: see section 13 14 15 16

axial skeleton

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20

basioccipital bone

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 04 05 06 07 22 23 24

vault of skull

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

clavicle

strong

regional

strong expression: see section 10 19

scapula

strong

regional

strong expression: see section 03 04 05 24

sternum

strong

regional

strong expression: see section 14 15

pelvic girdle skeleton

strong

regional

strong expression: see section 08 09 10 11 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31852
Entity Detected:	Tcam1, testicular cell adhesion molecule 1 ( MGI:1923120)
Sequence:	sense strand is shown >T31852 AGCAGAAGGACACCGTGCTCGCTATCACCATGTACCAGCCACCAGAGCAGGTGATACTGGACCTGCAGCC TGAATGGGTGGCCGTGGATGAAGCCTTCACAGTCACGTGTCATGTACCTAGTGTGGCACCCCTGCAGAGC CTCACCCTTACCCTCCTCCAGGGTGACCAAGAACTGCACAGAAAAGACTTCCTAAGTTTATCTTTGGTAT CCCAAAGAGCCGAGGTCACCGCCACTGTCAGAGCCCACCGGGACAATGACAGGCGTAATTTCTCCTGCCG AGCAGAACTGGATCTGAGCCCACATGGTGGGGGGTTGTTTCACGGCAGCTCAGCCACCAAGCAACTCCGG ATCTTTGAATTCTCTCAGAATCCCCAGATCTGGGTGCCTTCACTCCTGGAGGTTGGGAAGGCAGAGATTG TGAGCTGTGAGGTGACCAGAGTATTTCCAGCCCAGGAAGCTGTCTTCCGAATGTTCCTGGAAGACCAGGA GCTGAGCCCTTTCTCGTCCTGGAGGGAAGATGCAGCGTGGGCCAGTGCCACCATTCAGGCCATGGAGACT GGTGACCAGGAACTGACTTGCCTTGTGTCTCTGGGTCCCGTGGAGCAGAAAACAAGGAAACCAGTTTATG TCTACAGTTTCCCTCCACCAATCCTGGAGATAGAAGATGCTTACCCTCTGGCAGGGACGGACGTTAATGT GACCTGCTCAGGTCACGTGTTAACATCACCTAGCCCTACTCTTCGGCTTCAGGGATCCCTAAACCACTCT GCCCCTGGGAAGCCTGCCTGGCTTCTGTTTACTGCCAGGGAGGAAGATGATGGCCGGACTCTGTCCTGCG AGGCCTCTTTGGAGGTACAGGGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6741794), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60895. Forward Primer - name:060895_F_IRAW2_c05_Tcam1, sequence:AGCAGAAGGACACCGTGC; Reverse Primer - name:060895_R_SP6_IRAW2_c05_Tcam1, sequence:TGGCCCTGTACCTCCAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18423 same embryo
	EMAGE:18421 same embryo
	EMAGE:18420 same embryo
	EMAGE:18419 same embryo
	EMAGE:18418 same embryo
	EurExpress:euxassay_014376 same experiment
	MGI:4828628 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source