EMAGE mouse gene expression spatial database

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EMAGE:18512

Samd7 sterile alpha motif domain containing 7 ( MGI:1923203)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014465. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014465_01	euxassay_014465_02	euxassay_014465_03	euxassay_014465_04

euxassay_014465_05	euxassay_014465_06	euxassay_014465_07	euxassay_014465_08	euxassay_014465_09

euxassay_014465_10	euxassay_014465_11	euxassay_014465_12	euxassay_014465_13	euxassay_014465_14

euxassay_014465_15	euxassay_014465_16	euxassay_014465_17	euxassay_014465_18	euxassay_014465_19

euxassay_014465_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18512_wholemount_strong.wlz

18512_wholemount_moderate.wlz

18512_wholemount_weak.wlz

18512_wholemount_possible.wlz

18512_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18512_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 15 weak expression: see section 10 11 12 13 14

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 15 16 20 weak expression: see section 10 11 12 13 14 19

medulla oblongata alar plate mantle layer

strong

homogeneous

strong expression: see section 02 08 09 10 11 moderate expression: see section 12 13

medulla oblongata basal plate mantle layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 moderate expression: see section 12 13

rest of cerebellum mantle layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 moderate expression: see section 13 14

pons mantle layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 moderate expression: see section 13 14

midbrain mantle layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 moderate expression: see section 13 14

facial vii ganglion

strong

regional

strong expression: see section 01 02 03 15 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 02 03 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 moderate expression: see section 17 18

vagus x ganglion

strong

regional

strong expression: see section 04 12

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 13 14

spinal cord mantle layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09

dorsal root ganglion

strong

regional

strong expression: see section 01 02 03 04 05 08 09 10 11

neural retina

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37988
Entity Detected:	Samd7, sterile alpha motif domain containing 7 ( MGI:1923203)
Sequence:	sense strand is shown >T37988 GAGAGCAGAAACTGAAAGGGAACCTCCTTCTACTCACTCCTTCCATCGCCCAACCCTGATGGAGCAGCAG GTGCTGATGGGAGGAGGCAGGGCAAAGCTGATGAGAGACAGCACCTTATCTCCTAGCCACAGCAGCGTCT AGCCTGCAGTGGGGCCTACAAGGGGGAAGTTAGGATTTACCGGAGGGGAGGGGGAAAGTTATCTGTGTGT AACAAGTTGGAACTAGTGAGAGAGAGGTGATGATAAAGGTGGCTGTGTCCAGGCTTCCAACTTAACATTT GACAGAATGACTCCAAAGGGACACACACACACACACACACACACACACACACACACACACACACTGGCAA CAGTATGATCAGAGAGGGGACTGGCTGGCAAGCATAGGAGGTCTTATTTTGAATGGTCTGAATTGTAAAC CTTGAGAATGTGTCTCACGTGACACTCTCAGTTCAAGTAAATAAACCCTAGCAGAAAGCCTTTAAGATAG CCCACCCATTGTCGAGGGTGCTTGCCTTTGTATACCCCTCCCCTACAGTGTGCTGAACCAAAGAAACACA CCTTCCTAAGCACACCCATAAGGCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77137. Forward Primer - name:077137_F_cDNA_Samd7, sequence:GAGAGCAGAAACTGAAAGGGAA; Reverse Primer - name:077137_N_SP6_cDNA_Samd7, sequence:GGGCCTTATGGGTGTGCTTAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18514 same embryo
	EMAGE:18513 same embryo
	EMAGE:18511 same embryo
	EurExpress:euxassay_014465 same experiment
	MGI:4827865 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source