EMAGE mouse gene expression spatial database

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EMAGE:18635

Ranbp2 RAN binding protein 2 ( MGI:894323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016512. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016512_01	euxassay_016512_02	euxassay_016512_03	euxassay_016512_04

euxassay_016512_05	euxassay_016512_06	euxassay_016512_07	euxassay_016512_08	euxassay_016512_09

euxassay_016512_10	euxassay_016512_11	euxassay_016512_12	euxassay_016512_13	euxassay_016512_14

euxassay_016512_15	euxassay_016512_16	euxassay_016512_17	euxassay_016512_18	euxassay_016512_19

euxassay_016512_20	euxassay_016512_21	euxassay_016512_22	euxassay_016512_23	euxassay_016512_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18635_wholemount_strong.wlz

18635_wholemount_moderate.wlz

18635_wholemount_weak.wlz

18635_wholemount_possible.wlz

18635_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18635_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10

hypothalamus mantle layer

strong

regional

strong expression: see section 14 weak expression: see section 09 10 11 13

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 14 moderate expression: see section 15 weak expression: see section 11 13

telencephalon mantle layer

strong

regional

strong expression: see section 04 05 18 19 moderate expression: see section 03 weak expression: see section 20 21

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 16

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 16

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 14 moderate expression: see section 11 15 weak expression: see section 08 09 10 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38726
Entity Detected:	Ranbp2, RAN binding protein 2 ( MGI:894323)
Sequence:	sense strand is shown >T38726 AGGATTTTCAGTCAGAGCTTCGAAAAGTCTGTGAAGCTCAGAAATCCCAGAATGAGAAAGTGACTGATAG AGTTGGTATTGAGCATATAGGTGAGACTGAAGTAACAAACCCTGTTGGCTGTAAGTCTGAAGAACCTGAT TCTGATACCAAACACAGTAGCTCATCCCCTGTTTCTGGGACTATGGACAAACCTGTCGATTTGTCCACTA GAAAAGAAACTGACATGGAATTCCCAAGCAAAGGGGAAAACAAGCCTGTTTTGTTTGGATTTGGAAGTGG TACAGGCCTGTCATTTGCAGACTTGGCTTCAAGTAATTCTGGAGATTTTGCTTTTGGCTCTAAAGATAAA AATTTCCAGTGGGCAAATACTGGAGCAGCTGTGTTTGGAACACAGACAACAAGTAAAGGTGGCGAGGATG AAGACGGCAGTGATGAAGATGTAGTTCATAATGAGGACATCCACTTTGAACCTATAGTCTCCTTACCAGA GGTAGAAGTGAAATCTGGAGAGGAAGATGAAGAAGTTTTGTTCAAAGAGAGAGCCAAACTTTATAGGTGG GACAGAGATGTCAGTCAGTGGAAGGAGAGAGGTATTGGAGATATAAAGATTCTCTGGCATACAATGAAGA AGTATTATCGGATCCTAATGAGAAGAGACCAGGTTTTTAAAGTGTGTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104842. Forward Primer - name:104842_F_cDNA_Ranbp2, sequence:AGGATTTTCAGTCAGAGCTTCG; Reverse Primer - name:104842_N_SP6_cDNA_Ranbp2, sequence:GCACACACTTTAAAAACCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18633 same embryo
	EMAGE:18634 same embryo
	EMAGE:18632 same embryo
	EurExpress:euxassay_016512 same experiment
	MGI:4827605 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source