EMAGE mouse gene expression spatial database

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EMAGE:18740

Srd5a1 steroid 5 alpha-reductase 1 ( MGI:98400)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014142. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014142_01	euxassay_014142_02	euxassay_014142_03	euxassay_014142_04

euxassay_014142_05	euxassay_014142_06	euxassay_014142_07	euxassay_014142_08	euxassay_014142_09

euxassay_014142_10	euxassay_014142_11	euxassay_014142_12	euxassay_014142_13	euxassay_014142_14

euxassay_014142_15	euxassay_014142_16	euxassay_014142_17	euxassay_014142_18	euxassay_014142_19

euxassay_014142_20	euxassay_014142_21	euxassay_014142_22	euxassay_014142_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18740_wholemount_strong.wlz

18740_wholemount_moderate.wlz

18740_wholemount_weak.wlz

18740_wholemount_possible.wlz

18740_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18740_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

strong

regional

strong expression: see section 09 12 13

lower jaw molar

strong

regional

strong expression: see section 04 05 16 17

upper jaw incisor

strong

regional

strong expression: see section 09 12 13

upper jaw molar

strong

regional

strong expression: see section 04 17

bladder

moderate

regional

moderate expression: see section 09 10 11

urethra of male

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40382
Entity Detected:	Srd5a1, steroid 5 alpha-reductase 1 ( MGI:98400)
Sequence:	sense strand is shown >T40382 GACCTCAGCTATGGAGTTGGATGAGTTGCGCCTACTGGATGCGCTAGTCTACCTGGAGGGTTTCCTGGCT TTCGTGGCCTTCGTGGGGCTCCAGATGGTGGGCTCTTCCTACGGCCGCTACTCCTCGCAGTGGTCCGGCC GCCGAGTGCCCGCGCGACCTGCCTGGTTCCTACAGGAGCTGCCTTCAATGGCCTGGCCGCTGTACGAGTG CATCCGTCCTGCAGCCGCCCGACTGGGCAACCTGCCTAACCGCGTCCTGCTAGCTATGTTTCTGATCCAC TACGTGCAAAGGACGCTGGTTTTTCCAGTTCTGATCAGGGGAGGGAAGCCCACCCTGCTGTTCACCTTTG TCTTGGCCTTCCTGTTCTGCACCTTAAATGGCTACTTGCAGAGCCGATACTTGAGCCAGTTTGCGGTGTA TGCTGAAGACTGGGTAACCCATCCCTGTTTCCTGACAGGCTTTGCCCTGTGGTTAGTGGGCATGGTGATA AATATCCACTCAGACCACATCCTGCGGAATCTGAGAAAACCAGGGGAAACTGGATACAAAATACCCAGGG CTCTGCAAATTCACAGCCTCACCGGGCAGCATTTCCTACTGACTGAAAGTTTACATCGTGAACCACAGTT AGATCTGGCTTTTTGGAAGATGATTAAGGGGTGAAGGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93589. Forward Primer - name:093589_F_cDNA_Srd5a1, sequence:GACCTCAGCTATGGAGTTGGAT; Reverse Primer - name:093589_N_SP6_cDNA_Srd5a1, sequence:CAGCCTTCACCCCTTAATCATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18737 same embryo
	EMAGE:18742 same embryo
	EMAGE:18738 same embryo
	EMAGE:18741 same embryo
	EMAGE:18739 same embryo
	EurExpress:euxassay_014142 same experiment
	MGI:4828436 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source