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EMAGE:18747

Wscd2 WSC domain containing 2 ( MGI:2445030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747
"Pseudo-wholemount" of euxassay_014115. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014115_01 euxassay_014115_02 euxassay_014115_03 euxassay_014115_04
EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747
euxassay_014115_05 euxassay_014115_06 euxassay_014115_07 euxassay_014115_08 euxassay_014115_09
EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747
euxassay_014115_10 euxassay_014115_11 euxassay_014115_12 euxassay_014115_13 euxassay_014115_14
EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747
euxassay_014115_15 euxassay_014115_16 euxassay_014115_17 euxassay_014115_18 euxassay_014115_19
EMAGE:18747 EMAGE:18747 EMAGE:18747 EMAGE:18747
euxassay_014115_20 euxassay_014115_21 euxassay_014115_22 euxassay_014115_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18747Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18747_wholemount_strong.wlz
18747_wholemount_moderate.wlz
18747_wholemount_weak.wlz
18747_wholemount_possible.wlz
18747_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18747_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 20 21 22 23
forelimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 20 22 23
forelimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 20 22 23
forelimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 20 21 22 23
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 21
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 21
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 21
tarsus
moderate moderate
regionalmoderate expression: see section 20
fibula
moderate moderate
regionalmoderate expression: see section 21 22
tibia
moderate moderate
regionalmoderate expression: see section 21 22
femur
moderate moderate
regionalmoderate expression: see section 05 14 21 22
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 05 17
otic capsule
moderate moderate
regionalmoderate expression: see section 05 06 07 08 15 16 17
utricle
moderate moderate
regionalmoderate expression: see section 05 06 07 17 18
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 10 11 12
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 16 17 18 19 20 21 22
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 05 06 07
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
moderate moderate
regionalmoderate expression: see section 06 07 08 15 16
scapula
moderate moderate
regionalmoderate expression: see section 04 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39600
Entity Detected:Wscd2, WSC domain containing 2 ( MGI:2445030)
Sequence:sense strand is shown

>T39600
GGAGTATGACCCTTACACAGCCGAGATGCGCAGGACCATTGCCGCCTACATCCGAATGGTGGACACGGCA
CTACGTAGCCGCAACCTCACTGGGGTGCCTGATGCCTATGGCCCAAGATGATGGGCAGCCAGGGAGGGAC
CCAGCATCCGGTGCCCCTTGGCTTCAAAGAGCTGGCTTCCCTCTGGCACAAAGACAGCCCCAGGCTTCTG
CCTGCCTTCAGAAAGGCCCCTCTGAGGCCTCAGGAGGAGGAGATATCTAGACACCTTTCCCTGGAGAGTA
GGCTGCCTGGTTTGGAGTATCCCAGACCCTGCTATTGGTGGTGGGAGAAGGGAGAGGCTATCTGGCAGAG
GTTGGGAGGGTCTGGCTGTGGCAGCTGATGGACATTTTGCAAGCAATGACCCTCTTCTGACTGTGTGGGC
CCCTGCCTCTTCCCCTGAGCAGGCACCAGCTTTCTGATCTCTCTCAGGCCACCTCTGTGTGACAGACAGC
TGGCTGGGACCTGTCTCCGTGGCCTTGGGCGTCTCACTTCTGAGGACCATGGAGACTGGTGCTCTGGCAT
TACAGTGGGAACCGCCCCAGGCTAAAGCACATAAGAGGCCCAAGCCGGGTGTTGCCCCCCCTCCAGGACA
GCAACCCTGTCTTGTCTGCTCGTGCTGTCGTGTCTTGGGGATGAGGGTGACGTTGGGGTTCTTGATCCTT
CTTCCAAAGTCCCTTAGGTACCAAAAACCAACCCAACAGGTTGGCCATGTGCCACATACGGAAGCCCAGA
GGACCCAACATTGCAGGGTGGGACCTGAACTGGCTATCTTACACTGCCTGGATTGATCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 269762. Forward Primer - name:269762_F_cDNA_4933413A10Rik, sequence:GGAGTATGACCCTTACACAGCC; Reverse Primer - name:269762_N_SP6_cDNA_4933413A10Rik, sequence:GGGATCAATCCAGGCAGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18748 same embryo
 EMAGE:18744 same embryo
 EMAGE:18745 same embryo
 EMAGE:18746 same embryo
 EMAGE:18743 same embryo
 EurExpress:euxassay_014115 same experiment
 MGI:4829233 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS