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EMAGE:18806

Ica1 islet cell autoantigen 1 ( MGI:96391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806
"Pseudo-wholemount" of euxassay_014251. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014251_01 euxassay_014251_02 euxassay_014251_03 euxassay_014251_04
EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806
euxassay_014251_05 euxassay_014251_06 euxassay_014251_07 euxassay_014251_08 euxassay_014251_09
EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806
euxassay_014251_10 euxassay_014251_11 euxassay_014251_12 euxassay_014251_13 euxassay_014251_14
EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806
euxassay_014251_15 euxassay_014251_16 euxassay_014251_17 euxassay_014251_18 euxassay_014251_19
EMAGE:18806 EMAGE:18806 EMAGE:18806 EMAGE:18806
euxassay_014251_20 euxassay_014251_21 euxassay_014251_22 euxassay_014251_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18806Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18806_wholemount_strong.wlz
18806_wholemount_moderate.wlz
18806_wholemount_weak.wlz
18806_wholemount_possible.wlz
18806_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18806_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 05 06 07 16 17 18
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 13 weak expression: see section 07 14
thyroid gland
weak weak
regionalweak expression: see section 09 14
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 weak expression: see section 14 15 16 17 18
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 weak expression: see section 01 02 03 12 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 11 12 13 14 15 16 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 15 18 19 weak expression: see section 04 05 06 07 08 16 17 20
pons mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 06 07 08
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 17 18 19 weak expression: see section 06 07 08 16 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 19 20
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 16
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 16 17 18
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 weak expression: see section 09 10 13 14 15 16 17
neural retina
weak weak
regionalweak expression: see section 01 02 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6022
Entity Detected:Ica1, islet cell autoantigen 1 ( MGI:96391)
Sequence:sense strand is shown

>T6022
CCCACGCGTCCGCCCACGCGTCCGCTCCTGGGGGCCGGAGCACCAAGGTTTAATATAACATATCCTATCA
TGATTTTTCTCTGCCCCTTCTCAAATCATCGGCAGTAGAAAAGAGAAGAAAACATGTCAGGACACAAATG
TTATTCCTGGGAGTTGCAGGACCGGTTTGCTCAAGATAAGTCAGTTGTCAATAAGATGCAGCAGAAATAT
TGGGAGACCAAGCAGGCCTTTATCAAAGCCACGGGGAAGAAGGAAGATGAACATGTGGTTGCTTCTGATG
CAGACCTGGATGCCAAGCTGGAGCTGTTTCACTCAATCCAGAGAACCTGTCTGGACTTGTCTAAAGCAAT
CGTGCTCTATCAAAAGAGAATATGTTTCTTGTCTCAAGAGGAAAATGAACTGGGAAAGTTTCTTCGATCT
CAAGGCTTCCAAGACAAAACCCGAGCAGGAAAAATGATGCAAGCCACGGGCAAGGCCCTCTGCTTTTCCT
CCCAGCAAAGGTTGGCCTTGAGAAACCCTTTGTGTCGATTTCACCAAGAAGTAGAAACTTTTAGACATCG
GGCCATCTCTGATACCTGGCTGACAGTGAACCGCATGGAGCAGTACAGGACAGAATACAGAGGAGCGTTG
TTATGGATGAAGGACGTGTCTCAGGAACTTGATCCAGACCTCTACAAGCAAATGGAGAAGTTCAGGAAGG
TGCAGACACAAGTCCGCCTTGCGAAAAAAAACTTTGACAAGTTGAAGATGGATGTGTGTCAAAAGGTGGA
TCTTCTTGGAGCAAGCAGATGTAACCTCTTATCTCACATGCTAGCAACATACCAGACCACTCTGCTCCAC
TTTTGGGAGAAAACTTCTCACACCATGGCAGCCATTCATGAGAGCTTCAAAGGTTATCAACCATATGAAT
TCACAACGTTAAAGAGCTTACAAGACCCCATGAAGAAGCTAGTTGAGAAAGAAGGGAAGAAGACCTCCTG
GAGGGAAAACCGGGAGGCTGTGGCACCAGAGCCGAGGCAGTTAATTTCTTTGGAGGATGAGCACAAAGAT
TCATCTACTTATAAGACTGAAGAGGGAACAAGCGTTTTGTCTTCCGTAGACAAAGGTTCTGTACATGACA
CATGCTCAGGACCCATAGATGAACTATTAGACGGGAAACCCGAGGAAGCATGCCTGGGTCCCACGGCAGG
GACCCCAGAACCTGAAAGTGGGGACAAGGATGACCTCTTGCTGTTGAATGAGATCTTCAGCACTTCCTGC
CTGGATGAGGGGGAGTTCAGCAGAGAGTGGGCTGCAGTGTTTGGAGATGACCGGCTGAAGGAGCCAGCAC
CCATGGGAGCCCAGGGAGAGCCAGACCCCAAGCCCCAGATAGGCTCCGGATTCCTTCCATCACAGCTTTT
AGACCAAAATATGAAAGATCTCCAGGCCTCTCTGCAAGAGCCTGCCAAGGCTGCCTCGGACCTGACTGCC
TGGTTCAGCCTCTTTGCTGACCTCGACCCCTTATCAAACCCTGATGCTGTTGGGAAAACTGATAAAGAAC
ACGAATTGCTCAATGCATGAGTCTGCAACCTTCAACAGGGAGCCCTCGGGCCACTCCGCGGCACCTCATC
CAGGGCTTGCAGAAGTCTAATATGCTCAGTGTGCTGTTTTAATATTTACATGCCATTTTAATAAAACGAG
AGGGTCAAGGCCCTGTTTCTATCGCTATAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3487814 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487814 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18807 same embryo
 EMAGE:18804 same embryo
 EMAGE:18805 same embryo
 EurExpress:euxassay_014251 same experiment
 MGI:4825497 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS