EMAGE mouse gene expression spatial database

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EMAGE:18891

Gm7325 predicted gene 7325 ( MGI:3649059)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014154. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014154_01	euxassay_014154_02	euxassay_014154_03	euxassay_014154_04

euxassay_014154_05	euxassay_014154_06	euxassay_014154_07	euxassay_014154_08	euxassay_014154_09

euxassay_014154_10	euxassay_014154_11	euxassay_014154_12	euxassay_014154_13	euxassay_014154_14

euxassay_014154_15	euxassay_014154_16	euxassay_014154_17	euxassay_014154_18	euxassay_014154_19

euxassay_014154_20	euxassay_014154_21	euxassay_014154_22	euxassay_014154_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18891_wholemount_strong.wlz

18891_wholemount_moderate.wlz

18891_wholemount_weak.wlz

18891_wholemount_possible.wlz

18891_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18891_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 20 21 22 23

upper leg muscle

moderate

regional

moderate expression: see section 18 19 20 21 22

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 22 23

hand

moderate

regional

moderate expression: see section 21 22

foot

moderate

regional

moderate expression: see section 20 21

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 20 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

eye skeletal muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 18 19 20 21

tongue muscle

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39953
Entity Detected:	Gm7325, predicted gene 7325 ( MGI:3649059)
Sequence:	sense strand is shown >T39953 CCAGAAGAAAGCTGCACTGTAAAACTAATCCAGTTGAAAACTGGGGAGTACAGAGGTGCAGGTCCTGCCA TGCCCGTTCCATTGCTCCCGATGGTGCTTCGATCGCTGCTGTCCCGCCTGCTGCTGCCTGTTGCCCGCCT GGCCCGGCAGCACCTCCTGCCCTTGCTGCGCCGGCTGGCCCGCCGACTGAGCTCCCAAGACATGAGAGAG GCTCTGCTGAGCTGTCTGCTCTTTGTCCTCAGCCAGCAACAGCCACCGGATTCTGGAGAGGCCTCCAGAG TGGACCACTCCCAGAGGAAGGAGAGATTGGGCCCCCAGAAGTGAGGCCACGGGTCCTGGAAACAGCAACG CCCATCAAAGTACTTTTGGAGCCGGTTAGTCCAGGCGTCGGTCCGCACGCACGGGCATGGACGGCAGACT GCCCAGTGGGCGAAGACAGTCCGGGCTGAGTGCAAGAGGGCTCTGACCTGAACAGTTCCCACTCCCCTAG CTCCTAGCAGGCTACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235397. Forward Primer - name:164552_F_cDNA_LOC433110, sequence:CCAGAAGAAAGCTGCACTGTAA; Reverse Primer - name:164552_N_SP6_cDNA_LOC433110, sequence:TGTAGCCTGCTAGGAGCTAGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18890 same embryo
	EMAGE:18894 same embryo
	EMAGE:18895 same embryo
	EMAGE:18892 same embryo
	EMAGE:18893 same embryo
	EurExpress:euxassay_014154 same experiment
	MGI:4825117 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source