EMAGE mouse gene expression spatial database

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EMAGE:18927

Clasp2 CLIP associating protein 2 ( MGI:1923749)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014210. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014210_01	euxassay_014210_02	euxassay_014210_03	euxassay_014210_04

euxassay_014210_05	euxassay_014210_06	euxassay_014210_07	euxassay_014210_08	euxassay_014210_09

euxassay_014210_10	euxassay_014210_11	euxassay_014210_12	euxassay_014210_13	euxassay_014210_14

euxassay_014210_15	euxassay_014210_16	euxassay_014210_17	euxassay_014210_18	euxassay_014210_19

euxassay_014210_20	euxassay_014210_21	euxassay_014210_22	euxassay_014210_23	euxassay_014210_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18927_wholemount_moderate.wlz

18927_wholemount_weak.wlz

18927_wholemount_possible.wlz

18927_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18927_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

telencephalon

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

metencephalon

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 17

facial vii ganglion

weak

single cell

weak expression: see section 04 05 19 20 21

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 06 07 17 18

trigeminal v ganglion

weak

single cell

weak expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

weak

single cell

weak expression: see section 08 17

vestibulocochlear viii ganglion

weak

single cell

weak expression: see section 06 07 17

trigeminal v nerve

weak

single cell

weak expression: see section 08 09 16

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40340
Entity Detected:	Clasp2, CLIP associating protein 2 ( MGI:1923749)
Sequence:	sense strand is shown >T40340 ACCTAAAACACCTGGGAATCCTGTCAGCAGTGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGA GGTCCTTCTAAAGAAGGAGGGGCTGGAGCAGTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTC CTTCTGTTCAGATCTATTCTAGTCGAGAACTTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGA TGACAAACATGACTGGGACCAGCGTGCCAATGCGTGCATGTCCTGCAGCCTGGTAGCCAGTGAGGTTGAG AGAGCTCTGGCCTGGCCTCTGTGGAGTCACCTAGCTACTTATCAGCACCATTGTCATTGCACCCTGAGTC ATGAGGACTTGCCAGAGAAGAGATTGACTTCTCCTGTGAGCTCGGCATTTGTTCAACATTGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 178551. Forward Primer - name:178551_F_cDNA_Clasp2, sequence:ACCTAAAACACCTGGGAATCCT; Reverse Primer - name:178551_N_SP6_cDNA_Clasp2, sequence:GTCAATGTTGAACAAATGCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18926 same embryo
	EurExpress:euxassay_014210 same experiment
	MGI:4823891 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source