EMAGE mouse gene expression spatial database

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EMAGE:18996

N28178 expressed sequence N28178 ( MGI:2140712)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015915. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015915_01	euxassay_015915_02	euxassay_015915_03	euxassay_015915_04

euxassay_015915_05	euxassay_015915_06	euxassay_015915_07	euxassay_015915_08	euxassay_015915_09

euxassay_015915_10	euxassay_015915_11	euxassay_015915_12	euxassay_015915_13	euxassay_015915_14

euxassay_015915_15	euxassay_015915_16	euxassay_015915_17	euxassay_015915_18	euxassay_015915_19

euxassay_015915_20	euxassay_015915_21	euxassay_015915_22	euxassay_015915_23	euxassay_015915_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18996_wholemount_strong.wlz

18996_wholemount_moderate.wlz

18996_wholemount_weak.wlz

18996_wholemount_possible.wlz

18996_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18996_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 07 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 13 14 15 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15

rest of cerebellum mantle layer

weak

regional

weak expression: see section 05 06 13 14 15 16 17 18 19

pons mantle layer

weak

regional

weak expression: see section 06 07 09 10 11 12 13 14 15 16 17

midbrain mantle layer

weak

regional

weak expression: see section 05 06 07 09 10 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16 17 18 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 13 14 15 moderate expression: see section 08 09 16

neural retina

weak

regional

weak expression: see section 02 03 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63251
Entity Detected:	N28178, expressed sequence N28178 ( MGI:2140712)
Sequence:	sense strand is shown >T63251 TGGTCCCTAGTCTTCAAGTGGTCCAAAGCTCTAGGGACCCAAGAAGAACAGATACACTGGCAGGAATCAC TGTGTGTGAGGTGCTTTAGCCTAGGTGGCGGCCTCAGAGGCCGATGAGGATGCAGAAAAGAAAGCCCGGG CTTTTCCTACTTTCCAGCCCAACTCAGCCACAGACATAGAACTGTGGCATCATATGGAGGGCAAGCCACC CTCTTACATAGCCAGAGGAGCTCTGCAGAATGCCTACGCAGTTGTGCTTTTTAAGAAGAGACAACAAAGG CTCGAGAAGATTTGTATCTCAACTGTCAAGAAGTCCTAGTCAGCGTCAGAAGGGGGACAGTGTGTCCTTC TCCCGTCTCCCCAGTCGCTGCTTCTCCCTGATGCTCACTCCGTGTCTTCCCCTCACCTGAGAGGACCTTG TGGACAAGTGGAGAACGAACGCCATGGGTGTAGCAAGCTTGGCCTCCAGGCTTTGTGTAACCCATCACCC AGTTCTGAAGCTCTGGAGTCAGAACATGGTGTGGGTCAGCCCTAAGACCTAGGGATCCAGGTTCTGGATT TCTGTTTTCAACCAGATGACTTGGGTATGGGCAGTTCTGACAACTAGTTCTTCATGAGAGAACTGAGCAA AGTCCCGCAGAAAGCTTGCATTGTCTCCACTGCTGAAAGCACTTGTACTAAAGTGGATGGTTTCAGGAGA AGGAGCATGCTGCTCAGTGTTGAGAGGCTCAGATGCCATGGGCACCTCCAGCCAAGCCTTGCTAAGAACC CTGCATGCTCTCCTTCTGTAGGACCTGCCTTACCTAGAAGCAGGGTAGTATACTGCTGGAGACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73032. Forward Primer - name:073032_F_cDNA_N28178, sequence:TGGTCCCTAGTCTTCAAGTGGT; Reverse Primer - name:073032_N_SP6_cDNA_N28178, sequence:TGTCTCCAGCAGTATACTACCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18998 same embryo
	EMAGE:18993 same embryo
	EMAGE:18997 same embryo
	EMAGE:18995 same embryo
	EMAGE:18994 same embryo
	EurExpress:euxassay_015915 same experiment
	MGI:4826577 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source