EMAGE mouse gene expression spatial database

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EMAGE:19083

Mir770 microRNA 770 ( MGI:3691606)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019416. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019416_01	euxassay_019416_02	euxassay_019416_03	euxassay_019416_04

euxassay_019416_05	euxassay_019416_06	euxassay_019416_07	euxassay_019416_08	euxassay_019416_09

euxassay_019416_10	euxassay_019416_11	euxassay_019416_12	euxassay_019416_13	euxassay_019416_14

euxassay_019416_15	euxassay_019416_16	euxassay_019416_17	euxassay_019416_18	euxassay_019416_19

euxassay_019416_20	euxassay_019416_21	euxassay_019416_22	euxassay_019416_23	euxassay_019416_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19083_wholemount_strong.wlz

19083_wholemount_moderate.wlz

19083_wholemount_weak.wlz

19083_wholemount_possible.wlz

19083_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19083_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 04 05 06 09 10 11 12 13 18 19 20 weak expression: see section 07 08 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 04 05 06 moderate expression: see section 03 07 08 24

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 07 weak expression: see section 23 24

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 07 weak expression: see section 23 24

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 07 weak expression: see section 23 24

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 24

foot mesenchyme

moderate

regional

moderate expression: see section 06

fibula

moderate

regional

moderate expression: see section 02 03 04 05

tibia

moderate

regional

moderate expression: see section 03 04

femur

strong

regional

strong expression: see section 08 moderate expression: see section 03 04 05 06 07 09 23 24

otic capsule

moderate

regional

moderate expression: see section 10 11 18 19 20

nasal septum

weak

regional

weak expression: see section 11 12

hyoid

moderate

regional

moderate expression: see section 11 13 14 15 weak expression: see section 16

meckel's cartilage

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 16 moderate expression: see section 12 13 14 15 18 19 20 weak expression: see section 17 21 22 23

axial skeleton

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 11 12 17 18 19 20 weak expression: see section 21

basioccipital bone

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

basisphenoid bone

moderate

regional

moderate expression: see section 11 12 13 14 15 16

exoccipital bone

moderate

regional

moderate expression: see section 03 05 06 07 08 09 10 23 24

temporal bone

moderate

regional

moderate expression: see section 03 04 05 06 07 08 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 05 06 08 18 19 20 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 10 11 19 20

scapula

moderate

regional

moderate expression: see section 04 05 06 07 24

pelvic girdle skeleton

moderate

regional

moderate expression: see section 10 11 12 19 20

axial skeleton tail region

strong

regional

strong expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70186
Entity Detected:	Mir770, microRNA 770 ( MGI:3691606)
Sequence:	sense strand is shown >T70186 AGCACCACGTGTCTGGGCCACG
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-770-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19085 same embryo
	EMAGE:19084 same embryo
	EurExpress:euxassay_019416 same experiment
	MGI:4826378 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source