EMAGE mouse gene expression spatial database

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EMAGE:19163

Lmcd1 LIM and cysteine-rich domains 1 ( MGI:1353635)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002078. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002078_01	euxassay_002078_02	euxassay_002078_03	euxassay_002078_04

euxassay_002078_05	euxassay_002078_06	euxassay_002078_07	euxassay_002078_08	euxassay_002078_09

euxassay_002078_10	euxassay_002078_11	euxassay_002078_12	euxassay_002078_13	euxassay_002078_14

euxassay_002078_15	euxassay_002078_16	euxassay_002078_17	euxassay_002078_18	euxassay_002078_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19163_wholemount_strong.wlz

19163_wholemount_moderate.wlz

19163_wholemount_weak.wlz

19163_wholemount_possible.wlz

19163_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19163_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 12 13 14 15 16 17 18 19 moderate expression: see section 10 11

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 19

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

vibrissa

strong

regional

strong expression: see section 03 04 05 06 17 18 19

hypothalamus ventricular layer

weak

regional

weak expression: see section 09 10 11 12 13

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 09 10 11 12

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 14 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata alar plate ventricular layer

weak

regional

weak expression: see section 05 06 07 09 10 11 12 13

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 05 06 07 09 10 11 12 13

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 12 13 14 15 16 17

pons ventricular layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

ventral grey horn

moderate

regional

moderate expression: see section 07 08

spinal cord ventricular layer

moderate

regional

moderate expression: see section 05 06 07

viscerocranium

strong

regional

Expression in the turbinate bone.

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13

lower lip

strong

regional

strong expression: see section 04 05 06 07 08 16 17 18 moderate expression: see section 09 15

oral epithelium

strong

regional

strong expression: see section 04 05 moderate expression: see section 17 18 19 weak expression: see section 07 08 15 16

upper lip

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 moderate expression: see section 09 10 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4636
Entity Detected:	Lmcd1, LIM and cysteine-rich domains 1 ( MGI:1353635)
Sequence:	sense strand is shown >T4636 CCACGCGTCCGCGGCTGCACACAGGCAAAGGAACCTGAACTTGGCCATTCAGCAGCGCTGTCCAGGCTGC GCGCTCCCTCTGGCCCCTGCCTGAGAAGCCAGGTGCTGTTTCTGCACCGCCAGGATGGCAAAAGTGGCTA AGGACCTCAACCCTGGAGTGCAAAAGATGTCCTTGGGCCAGCAGCAGTCCGCAAGAGGAGTGGCTTGTCT GCGGTGCAAGGGAATGTGCTCCGGCTTCGAGCCTCACTCGTGGAGGAAAATATGCAAGTCTTGTAAATGC AGCCAGGAGGAGCACTGCCTAAGCTCTGACCTGGACGATGATCGGAAAATTGGGCGCCTGCTGATGGACT CCAAGTATGCCACTCTCACCGCCAGGGTGAAAGGCGGAGACGGCATCCGCATCTATAAGAGGAACCGCAT GATCATGACCAACCCCATCGCCACCGGCAAAGACCCCACCTTTGACACTATCACCTACGAGTGGGCTCCC CCAGGAGTCACCCAGAAACTGGGCCTGCAGTACATGGAACTCATCCCCAAGGAGAGGCAGCCTGTGACAG GCACTGAGGGTGCCCTCTACCGCCGACGCCAGCTCATGCATCAGCTCCCTATCTATGACCAGGACCCCTC TCGATGCCGTGGACTTGTGGAGAATGAGCTGAAAGCTATGGAGGAGTTCGTCAAGCACTACAAGAGCGAG GCCCTTGGGGTGGGCGAGGTGGCCCTCCCAGGGCAGGGAGGCTTGCCCAAGGAAGAAAACAAGACACAGG AAAAGCCAGAGGGCACAGAGACCACTGCCCCAACCACCAATGGCAGCCTGGGTGATCCATCCAAAGAAGT GGAATACGTCTGTGAGCTCTGCAAGGGAGCAGCCCCAGTTGACAGCCCTGTGGTCTACGCTGACAGGGCT GGATACAGCAAACAGTGGCACCCCACGTGCTTTCAGTGTATCAAGTGCTCAGAGCCACTGGTGGACCTCA TCTACTTCTGGAAGGATGGTGCACCCTGGTGTGGCCGCCACTACTGTGAGAGTGTGCGGCCCCGGTGCTC AGGCTGTGATGAGATCATCTTCTCAGAAGACTACCAGCGTGTGGAAGACCTGGCCTGGCATCGGAAGCAC TTCATTTGCGAGGGCTGTGAGCAGCTGCTGAGCGGCCGTGCGTACATCGTCACCAAGGGTCAGCTCCTGT GCCCCACATGCAGCAAGTCAAAACGCTCCTGAAAGGCTGCCCGCCCACAGCCAGCAGAGTCTATCGGATC CCACCAGGAGACTGAAGCCATCCTATGGGGCGGATGCCACGGCCAGCAAGCAGTGCATATTCTAGGTTGA GCAGTGGTAGATCTTAGAGGGTCAGTAGTTTCAAAAACAGAGGTATTCTAAGAAGTCTTAGAATGGATTT ATTGTTTCCTAGCTGCAACCAACTAAAGACACACTGGTATCCTGCCAGGGAGCCCTAGGAGAAATTTCCC AGAATGCATTTCTGAGGGATCCAATCAAGAAGTAGATGTAGGATATGCTTTTTTTTTTCTCTCGTGGACA TGGGATCTCTCCAGGGGGCAGGATGAGATTCTCAACTCCCATTTGTTTGCCTCTTTCTCAAGTGGAATCT GAATCTTCAATAAACAATGTTTTTGGCATGATAAACATATCAATAAAAGGTTTATGAATTTCACACACAC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5007525 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5007525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19164 same embryo
	EurExpress:euxassay_002078 same experiment
	MGI:4825944 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source