EMAGE mouse gene expression spatial database

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EMAGE:19230

Paqr6 progestin and adipoQ receptor family member VI ( MGI:1916207)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001862. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001862_01	euxassay_001862_02	euxassay_001862_03	euxassay_001862_04

euxassay_001862_05	euxassay_001862_06	euxassay_001862_07	euxassay_001862_08	euxassay_001862_09

euxassay_001862_10	euxassay_001862_11	euxassay_001862_12	euxassay_001862_13	euxassay_001862_14

euxassay_001862_15	euxassay_001862_16	euxassay_001862_17	euxassay_001862_18	euxassay_001862_19

euxassay_001862_20	euxassay_001862_21	euxassay_001862_22	euxassay_001862_23	euxassay_001862_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19230_wholemount_strong.wlz

19230_wholemount_moderate.wlz

19230_wholemount_weak.wlz

19230_wholemount_possible.wlz

19230_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19230_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 08 09 10

vibrissa

moderate

regional

moderate expression: see section 04 05 06 19 20 21 22

hypothalamus ventricular layer

weak

regional

weak expression: see section 11 12 13

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 11 12 13

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 weak expression: see section 12 13 14 15 16

midbrain ventricular layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16

spinal cord ventricular layer

weak

regional

weak expression: see section 10 11

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 moderate expression: see section 02 22 23

stomach

moderate

regional

moderate expression: see section 06 07 08 09 10 weak expression: see section 04 05

urethra of male

moderate

regional

moderate expression: see section 12 13

lung

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17 18 moderate expression: see section 11 12 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2530
Entity Detected:	Paqr6, progestin and adipoQ receptor family member VI ( MGI:1916207)
Sequence:	sense strand is shown >T2530 TGGCCTCGAGNCAGATTCGGACGAGGCCTGGACCCTGCCCCCTGCTGCAGGGTAGCCCACTGGAAGAGGG ACTCCAAGCTAAACAACAGTAAGGCCCATTGGCATGGAGGGAGGGAGAGGCCAGGTCTCACTGCCTCGGA AGAGCCCTGCCTGACATGGGCCTGAGAAGGTGGCGACTATTCTGACCCTGAAAGGGGTAGCTGAAGAGAA GAGGCTCTCTAGGGACAGGTGGAAGGACGGAAGACCCAAGAGGCAGGTGGTGAAGGTCTAAATTAACACA GGTACCAGGCGGGGAGCAGAGTGGGGATGTGAGGCCTCTCCTGGGGCTTCTGCCCAGCTGCCCCAGGACC TTGCTCACCTCAGGTCACCCTGAGATCTTGTTCCATCGGGGCCACATACCCACCCTGCCCCAGGGCCAGA CTGTGCCTCTTGTCAGCAGCTCTGTATCTACTCACTGCTGCCTGTCTTGCTCACCACTGTGACCAAGCCT TTGCCAATTTGTGTGTTTGGATGTGTCCCCGGTGAGATAATGGTTGTGGTGGGGATTG
Notes:	The probe template was PCR amplified from IMAGE:1381988 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1381988 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19232 same embryo
	EMAGE:19231 same embryo
	EMAGE:19233 same embryo
	EurExpress:euxassay_001862 same experiment
	MGI:4827046 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source