EMAGE mouse gene expression spatial database

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EMAGE:19234

Apod apolipoprotein D ( MGI:88056)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001865. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001865_01	euxassay_001865_02	euxassay_001865_03	euxassay_001865_04

euxassay_001865_05	euxassay_001865_06	euxassay_001865_07	euxassay_001865_08	euxassay_001865_09

euxassay_001865_10	euxassay_001865_11	euxassay_001865_12	euxassay_001865_13	euxassay_001865_14

euxassay_001865_15	euxassay_001865_16	euxassay_001865_17	euxassay_001865_18	euxassay_001865_19

euxassay_001865_20	euxassay_001865_21	euxassay_001865_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19234_wholemount_strong.wlz

19234_wholemount_moderate.wlz

19234_wholemount_weak.wlz

19234_wholemount_possible.wlz

19234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lumbar intervertebral disc

weak

regional

weak expression: see section 10 11 12

thoracic intervertebral disc

weak

regional

weak expression: see section 10 11 12

body-wall mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 04 05 06 07 18 19 20

diencephalon meninges

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain meninges

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord lateral wall

strong

homogeneous

strong expression: see section 11

spinal cord meninges

strong

homogeneous

strong expression: see section 08 09 10 12 13 14

cochlea

strong

regional

strong expression: see section 09 15 16 17 18

cochlear duct

strong

regional

strong expression: see section 06 07 08

viscerocranium

strong

regional

Expression in the turbinate bone.

lower lip

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 06 17 18 19 20 21 22

oral epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14

upper lip

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 14 15 16 17 18

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 13

axial skeleton sacral region

weak

regional

weak expression: see section 12 13

clavicle

weak

regional

weak expression: see section 07 08 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2533
Entity Detected:	Apod, apolipoprotein D ( MGI:88056)
Sequence:	sense strand is shown >T2533 TGGAAACTTCAGTCATCTGATCTGAGAGTTAGAAACTGACAGTGAGCACCAACTGAAGGCTTTTACAGGC TCCATCTCATTTTCCTCAGACTTCTAAGGCCTCTCCTGCAGCCACCCCACCCCAAGATGGTGACCATGCT GATGTTCCTGGCCACGCTGGCGGGTCTCTTCACCACAGCCAAAGGACAAAATTTCCATCTTGGGAAATGC CCGTCTCCTCCTGTGCAAGAGAATTTTGACGTGAAAAAGTATCTTGGAAGATGGTACGAAATTGAGAAGA TCCCAGCGAGCTTTGAGAAAGGAAACTGCATTCAAGCCAACTACTCGCTGATGGAGAACGGAAACATCGA AGTGCTAAACAAGGAGCTGAGTCCTGATGGAACCATGAACCAAGTAAAGGGTGAAGCCAAACAGAGCAAC GTCTCAGAGCCAGCCAAGCTGGAAGTCCAGTTCTTCCCGTTGATGCCACCGGCACCCTACTGGATCCTGG CCACCGATTATGAAAACTATGCCCTCG
Notes:	The probe template was PCR amplified from IMAGE:1382000 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1382000 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19235 same embryo
	EMAGE:19236 same embryo
	EMAGE:19237 same embryo
	EMAGE:19239 same embryo
	EMAGE:19238 same embryo
	EurExpress:euxassay_001865 same experiment
	MGI:4823188 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source