EMAGE mouse gene expression spatial database

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EMAGE:19243

Rhoj ras homolog gene family, member J ( MGI:1931551)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002084. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002084_01	euxassay_002084_02	euxassay_002084_03	euxassay_002084_04

euxassay_002084_05	euxassay_002084_06	euxassay_002084_07	euxassay_002084_08	euxassay_002084_09

euxassay_002084_10	euxassay_002084_11	euxassay_002084_12	euxassay_002084_13	euxassay_002084_14

euxassay_002084_15	euxassay_002084_16	euxassay_002084_17	euxassay_002084_18	euxassay_002084_19

euxassay_002084_20	euxassay_002084_21	euxassay_002084_22	euxassay_002084_23	euxassay_002084_24

euxassay_002084_25	euxassay_002084_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19243_wholemount_strong.wlz

19243_wholemount_moderate.wlz

19243_wholemount_weak.wlz

19243_wholemount_possible.wlz

19243_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19243_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 21 22 23 24 25 26 not examined expression: see section 20

mesenchyme

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

vertebral axis musculature

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

gland

moderate

homogeneous

moderate expression: see section 11

alimentary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

diencephalon meninges

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

hindbrain meninges

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord meninges

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14

urinary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

reproductive system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

respiratory system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

tail

moderate

homogeneous

moderate expression: see section 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5467
Entity Detected:	Rhoj, ras homolog gene family, member J ( MGI:1931551)
Sequence:	sense strand is shown >T5467 AGCGAGGGGGGAAACTCCGTTTAAAAGCCCTTCTCTCCTCCATATTAGCTGTGTGGTGGAAAACCGGGCA GGAGTTATCCAAATCCCACCGTGTCATTAGCCAGGCTGGGGATGGGGAGGGTAGAGTGAGATTCTCACGG GCTGCTGATATTTCTAGACTGCCAGGAAGTCCCGGGAAGTCTATGGACATGAGAGTTTCTTAACTCCAGC TCTACCCCCAACTCCACTTCTCCGTTGGGATTGGGAGAAAGGGACAGACCCTTTTCATCCCTCCTCCCAA GAGTGCCTTCCTTCTCCAGCATGGTGAGCGGCGGGGCTCGAGTGTGTCTGGGCTGTGCCTGGGAGAGCCC GGCCAGCCAGAGTCAGGCCGCCTGAGCTCCAGCCTGGGGCCGGTGCCACCGCTCCATGCACTGCAGAAGC CCCAGCAGCTGCTGCGGGAGCCGCCAACATGAGCTGCAGAGAGAGGACCGACAGCAGCTGCGGCTGCAAT GGACATGAGGAGAACAGGATCCTGAAGTGCGTGGTGGTCGGGGACGGCGCGGTGGGGAAGACCTGCTTGC TGATGAGCTACGCCAACGACGCCTTCCCAGAGGAATACGTGCCCACTGTGTTTGACCACTACGCAGTTAC CGTGACGGTGGGAGGCAAGCAGCACTTGCTTGGACTATACGACACTGCAGGACAGGAGGATTACAACCAG CTGCGGCCACTCTCTTACCCCAACACAGATGTGTTTCTCATCTGCTTCTCTGTCGTAAACCCAGCCTCTT ACCACAACGTTCAGGAGGAGTGGGTCCCGGAACTGAAGGACTGCATGCCTCATGTGCCTTATGTGCTCAT CGGAACCCAGATTGATCTCCGAGATGACCCCAAAACCTTGGCGCGTCTGCTGTACATGAAGGAGAAGCCC CTCACCTATGAGCACGGCGTGAAGCTGGCGAAAGCGATTGGAGCGCAGTGCTACTTGGAATGTTCTGCCC TGACCCAGAAGGGTCTCAAAGCGGTTTTTGATGAAGCCATCCTCACCATTTTCCACCCCAAGAAAAAAAA GAAAGGCTGCTTAGGGTGTCACGGCTGCTGTGCAATTATCTGAAGTTGCCTGACACCCATCTCCCCGCCA TGCAAGGGCATGGAGGGCAGCCAGTCTCTGTGACCCAACTCTAGCCAAAAAGGAGGGCGTGACCAGAAAG GAATTCCTGCATCCCAACGCCTGTCCCAGTGCCCTGCCAGCTTCCCAGCTCAGCATCCTGTAAGCCACTG CCACAGTCCTAAGCCAGCCAGACCATGAACACTGTGTATGGAGAATGCAGAGTCACATTCAGACCATATG CTGGTGAGGTGTTGGAAGCCGAGTGACAATCCATCTGGTATTTCATATCTTCTCTCCCACGGTTATGGAC CAACAGAGAGTCATGACGGGAAAACCAAGCACTCTGTCCTGCACTGGTGGCCTTAGTTCATGTCTTCACC AAACTTGGGAATACAACACCAACATTTTTTTTCTGAATCAGTTCGTTCTTTTACCCAAGCACCTTAATGC TCATTTCTATCGCAGTTTCCCAATGTCCAATCACTTGAGCCTTACAAATCCACATCAAAATAATTGAAAG ACAAGCTGGATTGTGTTCACACCAACTGACGGTGGATCTCTGCCAATGTTCTTAGAGCCATCATGTACCT CTGAATGCCTAATTACAAAAAAAAAACTTTTCTAAACTTACAGACCTATAATGTTTTTAAACTTTTTAAA AGAAAACATCCTGTGAGTTACAGCTCCCAAGTTTGGCCAAAGATCATCCCACAAATGGTTCAGAAGATAG GGGGACTGCTCTTGCAAAACCCTACTGTTATTCAAACCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5373358 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5373358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19240 same embryo
	EMAGE:19242 same embryo
	EMAGE:19241 same embryo
	EurExpress:euxassay_002084 same experiment
	MGI:4827735 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source