EMAGE mouse gene expression spatial database

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EMAGE:19285

Fam5c family with sequence similarity 5, member C ( MGI:2443035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002098. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002098_01	euxassay_002098_02	euxassay_002098_03	euxassay_002098_04

euxassay_002098_05	euxassay_002098_06	euxassay_002098_07	euxassay_002098_08	euxassay_002098_09

euxassay_002098_10	euxassay_002098_11	euxassay_002098_12	euxassay_002098_13	euxassay_002098_14

euxassay_002098_15	euxassay_002098_16	euxassay_002098_17	euxassay_002098_18	euxassay_002098_19

euxassay_002098_20	euxassay_002098_21	euxassay_002098_22	euxassay_002098_23	euxassay_002098_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19285_wholemount_strong.wlz

19285_wholemount_moderate.wlz

19285_wholemount_weak.wlz

19285_wholemount_possible.wlz

19285_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19285_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 22 23 24

medulla oblongata alar plate mantle layer

strong

homogeneous

strong expression: see section 08 09 10 11 13 14 15 16 17 moderate expression: see section 12 18

medulla oblongata basal plate mantle layer

strong

homogeneous

strong expression: see section 08 09 10 11 13 14 15 16 17 moderate expression: see section 12 18

rest of cerebellum mantle layer

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 12

pons mantle layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18 19 moderate expression: see section 12

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 18 19 moderate expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5486
Entity Detected:	Fam5c, family with sequence similarity 5, member C ( MGI:2443035)
Sequence:	sense strand is shown >T5486 CCACGCGTCCGACAGACGTTCGCTGTCTAGGTGTTGAGGGAAAGCTGAGAGGATGACGGCTCTAAGTCCC CAGTGGAAGCATGATATGGCGGCGCAGAGCTGGTGCTGAACTGTCCTCTCTGATGGCTCTATGGGAGTGG ATAGTACTGAGTCTTCATTGCTGGGTTTTAGCGGTTGCTGCTGTCTCTGACCAGCATGCCACAAGCCCCT TCGACTGGCTCCTCTCTGATAAGGGACCCTTCCATCGCTCACAGGAGTACACAGATTTTGTGGACAGAAG CCGACAGGGATTTAGCACAAGATACAAGATATACAGGGAGTTTGGTCGTTGGAAGGTAAATAACCTTGCA GTTGAGAGAAGAAATTTCCTTGGTTCTCCTCTGCCTCTTGCCCCTGAGTTCTTCCGCAACATAAGACTTT TGGGACGACGACCTACTCTACAGCAAATCACAGAAAATCTTATCAAGAAATATGGGACTCATTTCTTGCT ATCAGCTACTCTGGGAGGAGAGGAGTCACTCACAATTTTTGTGGACAAGCGGAAGCTGAGCAAAAGATCT GAAGGAAGTGAAACCAGCACCAATAGTTCGTCTGTTACCCTGGAGACCTTACATCAATTGGCTGCTTCCT ATTTTATTGACAGGGACAGCACCCTTCGCAGACTTCACCATATCCAAATTGCATCCACTGCCATCAAGGT GACAGAAACACGGACAGGTCCTCTTGGCTGCAGTAACTATGACAACCTAGATTCTGTCAGCTCTGTTCTG GTTCAGAGTCCTGAGAATAAAATTCAGTTGCAAGGGCTTCAAGTTCTCCTTCCCGACTACCTTCAGGAAC GTTTTGTACAGGCAGCACTGAGCTACATTGCTTGCAATTCTGAGGGCGAGTTTATCTGCAAGGATAATGA CTGCTGGTGCCACTGTGGTCCAAAATTCCCAGAATGTAATTGCCCTTCCATGGATATTCAAGCCATGGAA GAGAATCTTCTTCGAATAACAGAAACATGGAAAGCCTACAACAGTGACTTTGAAGATTCAGATGAATTCA AGTTCTTTATGAAAAGACTACCCATGAATTATTTTCTCAACACTTCAACTATAATGCATTTGTGGACAAT GGATTCTAATTTTCAGCGCCGCTACGAACAACTGGAGAACAGCATGAAACAGCTTTTCCTGAAGGCACAC AGAATTGTACACAAGCTCTTTAGCCTTAGCAAACGGTGCCACAAGCAGCCTCTCATCAGCCTGCCGAGAC AGAGAACATCAACCTACTGGCTCACTCGCATCCAGTCTTTTCTCTACTGCAATGAGAATGGCCTTCTGGG AAGCTTCTCCGAGGAAACCCACTCCTGCACATGCCCCAACGACCAGGTAGTGTGCACAGCCTTTCTGCCC TGTACTGTGGGCGATGCCTCTGCCTGCCTTACCTGTGCACCTGACAACCGTACCCGGTGTGGCACCTGCA ACACAGGCTACATGCTGAGCCAGGGACTCTGCAAGCCTGAAGTCGCTGAGTCAACCGATCACTACATTGG CTTTGAGACCGACCTACAAGACCTAGAGATGAAATACCTGTTGCAGAAAACAGATAGACGAATAGAAGTA CATGCCATCTTTATCAGCAATGACATGCGCCTCAACAGCTGGTTTGATCCTTCCTGGAGAAAGCGGATGC TCCTGACCTTGAAGAGTAACAAATACAAGTCCAGTCTGGTCCACATGATCTTGGGTCTCTCTTTACAGAT TTGCTTAACTAAAAACAGCACGTTGGAGCCAGTGCTGGCTGTTTATGTCAATCCCTTTGGAGGCAGCCAC TCCGAGAGCTGGTTTATGCCTGTGAGTGAGAGCAGCTTTCCAGACTGGGAGCGGACTAAGCTGGACCTCC CTCTACAGTGCTATAACTGGACACTGACTCTGGGAAACAAATGGAAGACATTTTTTGAGACAGTACACAT CTACCTGAGGAGTCGCATCAAGGCAAATGGCCCCAACAGCAATGAGAGCATTTATTATGAGCCTTTGGAG TTTATTGACCCATCCCGGAACCTGGGCTACATGAAAATCAACAACATCCAAGTGTTTGGCTACAGCATGC ACTTTGACCCTGAAGCCATTCGGGACCTGATTTTGCAGCTGGACTACCCTTATACTCAGGGATCCCAGGA CTCGGCACTTTTGCAGCTACTAGAGATAAGAGACCGTGTAAATAAACTCTCCCCACCCGGTCAGCGCCGC CTGGATCTGTTTTCTTGCTTGCTTCGTCATAGACTCAAGCTGTCTACTAGTGAGGTGGTGAGAATTCAGT CTGCTCTTCAGGCATTTAATGCCAAGTTGCCAAACACAGTGGATTATGACACAACCAAATTATGTAGTTA ACCATAAATGTCAAGCACAACCCAAAATCTTGAAGGAGTTTTTACAGTGCTTTTGTGGAAGTTTATGTTT GGAAAAGTAAATTTAAATTGTCTTTTCAATATCTGTCTTATATCAGTCAATAACGTTGGATGGCAATTTA CACACATGAACTTGCTGACAATGAATATATTATACTACAGTTTTGGTTTATGAATGAAATAAATACTGAC ACCAGTCTAGAAGACATTCTACTTTTTACAATAAATTTCATTTGTAATTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5400263 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5400263 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19281 same embryo
	EMAGE:19284 same embryo
	EMAGE:19283 same embryo
	EMAGE:19282 same embryo
	EMAGE:19280 same embryo
	EurExpress:euxassay_002098 same experiment
	MGI:4824750 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source