EMAGE mouse gene expression spatial database

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EMAGE:19307

Gstt3 glutathione S-transferase, theta 3 ( MGI:2143526)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009260. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009260_01	euxassay_009260_02	euxassay_009260_03	euxassay_009260_04

euxassay_009260_05	euxassay_009260_06	euxassay_009260_07	euxassay_009260_08	euxassay_009260_09

euxassay_009260_10	euxassay_009260_11	euxassay_009260_12	euxassay_009260_13	euxassay_009260_14

euxassay_009260_15	euxassay_009260_16	euxassay_009260_17	euxassay_009260_18	euxassay_009260_19

euxassay_009260_20	euxassay_009260_21	euxassay_009260_22	euxassay_009260_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19307_wholemount_strong.wlz

19307_wholemount_moderate.wlz

19307_wholemount_weak.wlz

19307_wholemount_possible.wlz

19307_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19307_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 06 18

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 06 07 18 19

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 06 07 18 19

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 06 07 18

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16

thyroid gland

strong

regional

strong expression: see section 08 09 12 13

epidermis

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

stomach

strong

regional

strong expression: see section 05 06 07 08 moderate expression: see section 03 04

hindgut

moderate

regional

moderate expression: see section 09 10 11

rectum

strong

regional

strong expression: see section 09

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

bladder

strong

regional

Expression in the fundus region.

kidney calyx

strong

regional

strong expression: see section 03 04 05 06 11 12 13 14

kidney pelvis

strong

regional

strong expression: see section 05 06 12 13

urethra of male

strong

regional

strong expression: see section 09 10

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T600
Entity Detected:	Gstt3, glutathione S-transferase, theta 3 ( MGI:2143526)
Sequence:	sense strand is shown >T600 TCCTCGAGNCTGTTGGCCTACTGGTGCCGTTCCTGCTCGCCGTTGCGGCTGCTCCGGCTTCTGTCCCCAG AGGCAGTTCACCCGAGGCTAGTTCCCGAGGTCTGCCAGCGGCTACTCCCACAGCCTCCGCCATGGGTCTG GAGCTCTACCTGGGCCTGATGTCCCAACCCTGCCGTGCTGTCTACATCTTCGCCAAGAAGAACGGCATCC CCTTCCAGCTGCGTACCATAGAGCTGCTTAAAGGTCAGCAGTACACAGATTCCTTCGCCCAGGTGAACCC TTTGAGGAAGGTGCCAGCTTTGAAGGATGGGGACTTCGTCTTGGCAGAGAGTGTGGCCATCTTGTTGTAT CTGAGTAGAAAGTACAAGGCCCCCGACCACTGGTATCCTCAGGATCTGCAGACTCGAGCTCGTGTAGACG AGTACCTGGCATGGCAGCACACAGCCCTGCGGAGTTGTTGCACCAGGGCCATGTGGCAGAAGATGATGTT CCCTGTGTTCCTGGGACAGCCCGTACCTCCTGAGATGCTGGCATCCACCTTGGCTGAACTGG
Notes:	The probe template was PCR amplified from IMAGE:1885751 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885751 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19306 same embryo
	EMAGE:19309 same embryo
	EMAGE:19308 same embryo
	EurExpress:euxassay_009260 same experiment
	MGI:4825268 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source