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EMAGE:19343

Lect1 leukocyte cell derived chemotaxin 1 ( MGI:1341171)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343
"Pseudo-wholemount" of euxassay_002141. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002141_01 euxassay_002141_02 euxassay_002141_03 euxassay_002141_04
EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343
euxassay_002141_05 euxassay_002141_06 euxassay_002141_07 euxassay_002141_08 euxassay_002141_09
EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343
euxassay_002141_10 euxassay_002141_11 euxassay_002141_12 euxassay_002141_13 euxassay_002141_14
EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343
euxassay_002141_15 euxassay_002141_16 euxassay_002141_17 euxassay_002141_18 euxassay_002141_19
EMAGE:19343 EMAGE:19343 EMAGE:19343 EMAGE:19343
euxassay_002141_20 euxassay_002141_21 euxassay_002141_22 euxassay_002141_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19343_wholemount_strong.wlz
19343_wholemount_moderate.wlz
19343_wholemount_weak.wlz
19343_wholemount_possible.wlz
19343_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 23
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 06 07 22 23
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 04 05 06 07 08 22 23
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 04 05 06 07 08 22 23
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 08 22 23
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 22 23
leg
strong strong
regionalstrong expression: see section 01 02 03
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 21 22 23
cochlea
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 16 17 18
cochlear duct mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23
nasal septum mesenchyme
strong strong
regionalstrong expression: see section 11 12 13 14 15
axial skeleton
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
chondrocranium
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23
viscerocranium
strong strong
regionalExpression in the turbinate bone.
pectoral girdle and thoracic body wall skeleton
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
axial skeleton tail region
strong strong
regionalstrong expression: see section 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5528
Entity Detected:Lect1, leukocyte cell derived chemotaxin 1 ( MGI:1341171)
Sequence:sense strand is shown

>T5528
GGAACAGTTAGGACAGTGGGATAGGGTCTTCGCGTATCTTACTTCTCCATCTTCAGCCATGACAGAGAAC
TCAGACAAAGTTCCTATCACCATGGTAGGGCCTGAGGACGTTGAGTTTTGCAGTCCCCCGGCGTACACCA
CCGTCACCGTGAAGCCCTCCGGGAGCCCAACACGGCTGCTCAAGGTAGGAGCTGTGGTCCTCATTTCTGG
CGCGGTACTGCTGCTCTTCGGGGCCATCGGGGCCTTCTACTTCTGGAAGGGGAATGACAATCACATTTAC
AATGTTCATTACAGTATGAGTATCAATGGGAAACTACAAGATGGGTCAATGGAAATAGATGCTGTGAACA
ACTTGGAGACCTTTAAAATGGGAAGCGGAGCGGAAGAAGCAATTGAAGTCAACGATTTTAAAAATGGCAT
CACTGGGATCCGTTTTGCTGGAGGAGAGAAGTGCTACATCAAAGCACAGGTGAAGGCTCGCATCCCTGAG
GTGGGCACAGTGACCAAGCAGAGCATCTCTGAACTGGAAGGCAAGATCATGCCAGCTAACTACGAAGAGA
ACTCGCTGATTTGGGTGGCCGTGGACCAGCCTGTGAAGGACAGCAGCTTCTTGAGCTCTAAAATCCTTGA
ACTCTGTGGCGACCTGCCGATTTTCTGGCTTAAGCCCATGTATCCAAAAGAAATCCAGAGAGAGAGAAGA
GAAGTCGTGAGAAACAGTGCTCCCTCTACCACAAGAAGACCACACAGCGAACCTCGAGGTAACGCAGGCC
CTGGAAGACTGAGTAACGGAACCAGACCCAATGTTCAGGACGACGCAGAACCTTTCAACCCTGACAATCC
TTACCACCAGCAGGAAGGAGAAAGCATGACATTTGACCCTAGACTGGACCATGAAGGGATCTGCTGTATA
GAATGCAGGCGGAGCTACACCCACTGCCAGAAGATCTGTGAACCACTGGGAGGCTACTATCCATGGCCTT
ACAATTACCAAGGATGCCGCTCGGCCTGCAGAGTCGTCATGCCATGCAGCTGGTGGGTGGCCCGCATCTT
GGGCATGGTGTAAATCCAGTTCATCTATCAGGACTGCCAAGCAAGAATCGATATGAGAGTTGAGAACCAA
AGAAGCATAGAGCATGTCCATGCCAAGAGACCACAAAGTATTTTATATTCAACCTGAATAATGTTATCCT
AAACGCTGTTGTGCACCACTGTGTGCAAATGTGCTGAAAGGGTAGTTTAACTCTACAATTATTATCATTT
TACGACTTGCTTTACCAATAAAGCCTACTGCAAGTCTTTAAAAGCTACTTTTGAAATAAATATGAAAACT
TAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6439864 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6439864 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19347 same embryo
 EMAGE:19346 same embryo
 EMAGE:19345 same embryo
 EMAGE:19344 same embryo
 EMAGE:19342 same embryo
 EurExpress:euxassay_002141 same experiment
 MGI:4825896 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS