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EMAGE:19419

Tes testis derived transcript ( MGI:105081)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419
"Pseudo-wholemount" of euxassay_004840. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004840_01 euxassay_004840_02 euxassay_004840_03 euxassay_004840_04
EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419
euxassay_004840_05 euxassay_004840_06 euxassay_004840_07 euxassay_004840_08 euxassay_004840_09
EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419
euxassay_004840_10 euxassay_004840_11 euxassay_004840_12 euxassay_004840_13 euxassay_004840_14
EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419
euxassay_004840_15 euxassay_004840_16 euxassay_004840_17 euxassay_004840_18 euxassay_004840_19
EMAGE:19419 EMAGE:19419 EMAGE:19419 EMAGE:19419
euxassay_004840_20 euxassay_004840_21 euxassay_004840_22 euxassay_004840_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19419Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19419_wholemount_strong.wlz
19419_wholemount_moderate.wlz
19419_wholemount_weak.wlz
19419_wholemount_possible.wlz
19419_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19419_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
humerus
strong strong
regionalstrong expression: see section 04 16 17
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 07 08 09 11 12
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 14 15 16 17 moderate expression: see section 07
brain
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
pituitary gland
moderate moderate
regionalmoderate expression: see section 11 12 13
adenohypophysis
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 18 19 20 21
facial vii ganglion
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 03 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 03 04 05 06 15 16 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 05 14
spinal cord
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 11 12 13
not examined not examined
regionalnot examined expression: see section 02 03 21 22
extrinsic ocular muscle
strong strong
regionalstrong expression: see section 02 03 04 20 21 22 23 moderate expression: see section 01 19
neural retina
strong strong
regionalstrong expression: see section 02 03 21 22
esophagus
moderate moderate
regionalmoderate expression: see section 08 09
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08
hindgut
strong strong
regionalstrong expression: see section 11 13 moderate expression: see section 12
rectum
strong strong
regionalstrong expression: see section 11
midgut
strong strong
regionalstrong expression: see section 09 10 11 13 14 15 16 moderate expression: see section 08 12
midgut loop
strong strong
regionalstrong expression: see section 09 10 11 13 14 15 moderate expression: see section 05 06 07 12
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 14
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 17
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 14
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 17 18
renal medullary interstitium
moderate moderate
homogeneousmoderate expression: see section 04 05 06 07 13 14 15 16
left lung
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08
right lung
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20
trachea
moderate moderate
regionalmoderate expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6504
Entity Detected:Tes, testis derived transcript ( MGI:105081)
Sequence:sense strand is shown

>T6504
GCCGCGCCGGAGTTCCCGCAGCGGAGATCGCGGACGCGGGACTGAGCCCCTCCGACGCCCAGTATGGACC
TGGAAACCAAAATGAAGAAGTGGAACTATGGACACCAAACGAGGTGATTTATGGGAAATACTTCGAGCTG
TGCCTGGCCGTGCAGATGCTTGCTGCTGTTCCCACTACGATCCCTTGTAGGCCCATTCCTGTGAAAGCAG
TGGACGTTTTGGGGGGCAGGATACTATGTATATAACAGGCAATCTCTCTGCTCTGGATCAAGAGGGTCAC
CTGAATTGTTGCCAGCGTATCTGGTTTCTTGACATGATTATGGAGAAATTGGTGCCACTTCCATTTCTAA
CTCTAAACTAAACAGAGAGAAGAAGCAGGGGCTTGGAGACTCGGGTCATGGAAATATGGGCTTAGGTCAT
GAGCAAGGATTTGGAGCCCCCTGTCTAAAATGCAAAGAGAACTGTGAAGGATTTGAACTACACTTCTGGA
GGAAAATATGTCGTAACTGCAAATGTGGTCAAGAAGAGCATGACGTCCTCCTGAGCAATGAGGAGGACCG
GAAAGTGGGGAGACTCTTTGAGGACACTAAGTACACCACCCTGATCGCCAAGCTGAAGTCAGATGGCATT
CCCATGTACAAACGCAATGTTATGATCTTGACCAATCCAGTGGCTGCCAAGAAGAACGTCTCCATCAACA
CAGTTACCTATGAGTGGGCTCCCCCAGTTCAAAACCAGGCTCTGGCCAGGCAGTACATGCAGATGCTGCC
TAAGGAGAAGCAGCCAGTGGCAGGTTCAGAGGGGGCACAGTACCGGAAGAAGCAGCTGGCGAAACAGCTC
CCGGCTCATGACCAGGATCCTTCTAAGTGCCATGAGTTGTCTCCCAAAGAGGTGAAGGAGATGGAGCAGT
TTGTGAAGAAATACAAGAGTGAGGCCCTGGGAGTGGGTGACGTGAAGTTTCCCTCTGAGATGAATGCTCA
GGGTGACAAGGTGCACAACCCTGCGGGGGACAGACACGCCCCAGCAGCAGTGGCTTCCAAGGACAAGTCT
GCAGAATCCAAGAAGACCCAGTATTCCTGTTACTGCTGCAAACACACCATGAACGAAGGCGAGCCGGCCA
TCTATGCTGAAAGGGCTGGCTACGATAAGCTCTGGCACCCGGCGTGTTTTATCTGCAGCACCTGTGGTGA
ACTCCTGGTCGACATGATTTACTTCTGGAAGAATGGGAAGCTGTACTGTGGCAGACATTACTGTGACAGT
GAGAAGCCCCGCTGTGCTGGCTGTGACGAGCTGATATTCAGCAATGAATATACCCAAGCAGAAAACCAGA
ATTGGCACCTGAAGCACTTCTGCTGCTTTGACTGCGACCACATCCTAGCAGGGAAAATCTACGTGATGGT
CACTGACAAGCCTGTGTGCAAGCCCTGCTATGTGAAGAACCATGCTGTGGTGTGTCAAGGATGCCACAAT
GCCATCGACCCGGAAGTGCAGCGAGTAACGTACAACAACTTCAGCTGGCACGCGTCCACAGAGTGTTTCC
TGTGCTCTTGCTGCAGCAAGTGTCTTATCGGGCAGAAGTTTATGCCCGTAGAAGGGATGGTTTTCTGTTC
CGTGGAGTGTAAGAGGATGATGTCCTAAGAGGAGTGTACCAAGCCGTATGGAGCCATAGCTGTCCACAGT
GGCCTGCATTTCATGATAAACTGCAGTTTGAAAAAAAAAATAAAAGGAAAAAAACCCTATATAACAAACC
AGAATATTTTGTCCAATATTTTTTCTTTTTAACCTTATGAATTTTGTCTGTCTTAATTTTAAATGACTTT
TCATGATTTAAAAAAAACAGTAAAGAATTGTAGCTTTTATTTCTATACATTAATTAAATCTTTGAATTGG
AAGCTTGAATTTAATCAGTCCTTATAACTGCATACTCCTAGTGCCAAATTTATATTAAGTGATAGATATG
AAATCACTTAAAATTAGTGTCCTGGGAGAGATGACCATTTTAACTTCTATGCTTATTTCACATGGGAAAT
GTAAAGGAAATTGCATGTTGCCTGACATGACGCTGTGCACATAGCCTGCCTCATCGATTGATGACGAGGT
GGCATCAGCTCGGTGAATTCTTCGGGCAGACAGCGTGGTCCACGATGGGCGTTCCTATGCCTAAGCCTTT
GCATCCCCTGGTGAGGCAGAGGGGCCTCTCTCACTGCGTCTCTCTCTGTTTACCTCACCTCCCCATGTTC
GCTTTTGCATCTAGTGACCGCCATCTGTGGCCTTGCATATTTTCTATGTGTGTGGCTGGAACAATATTGC
ACTTTGCTCTTAGAGATTATTATTTTTTGCCCAGAACATAAAAGTAGGCCATTCATTTAGTTATTTTTTT
TGAAACCCAAAGAATATGTATAGGTTTTGGTACCCAGGGAATTATAACTTGTGCTAATATCACCTCTCTG
CCATGCCTTGACTCAATTTTGCATGATTTACGGTGTTACCAGATGGATCCTCTCTTGTGCTACCTCTGAG
CTTGCTGTAGACACAAAGGCTATTTTCTTTGCCTGTGCACTCCCATCCTTGGGTATTTTTAATGTTTTCC
CTTGTATGCATTCATACATAACTATGCACTATAAATATTAAATGGAATGACTTATGTTATTGAAAATAAA
GTCTCTTTCACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3589585 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589585 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19424 same embryo
 EMAGE:19422 same embryo
 EMAGE:19423 same embryo
 EMAGE:19421 same embryo
 EMAGE:19420 same embryo
 EurExpress:euxassay_004840 same experiment
 MGI:4828661 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS