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EMAGE:19423

Dusp1 dual specificity phosphatase 1 ( MGI:105120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423
"Pseudo-wholemount" of euxassay_005013. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005013_01 euxassay_005013_02 euxassay_005013_03 euxassay_005013_04
EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423
euxassay_005013_05 euxassay_005013_06 euxassay_005013_07 euxassay_005013_08 euxassay_005013_09
EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423
euxassay_005013_10 euxassay_005013_11 euxassay_005013_12 euxassay_005013_13 euxassay_005013_14
EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423 EMAGE:19423
euxassay_005013_15 euxassay_005013_16 euxassay_005013_17 euxassay_005013_18 euxassay_005013_19
EMAGE:19423 EMAGE:19423 EMAGE:19423
euxassay_005013_20 euxassay_005013_21 euxassay_005013_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19423Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19423_wholemount_strong.wlz
19423_wholemount_moderate.wlz
19423_wholemount_weak.wlz
19423_wholemount_possible.wlz
19423_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19423_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 09 10
forearm mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 06
upper leg mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
femur
strong strong
regionalstrong expression: see section 07 moderate expression: see section 21
brain
weak weak
homogeneousweak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 04 14
trigeminal v ganglion
weak weak
regionalweak expression: see section 03 04 05 06 14 15 16 17 18 19
vagus x ganglion
weak weak
regionalweak expression: see section 05 13
spinal cord
weak weak
homogeneousweak expression: see section 05 06 07 08 09 10 11 12
neural retina
weak weak
regionalweak expression: see section 01 02 03 20 21
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 12 13 14 moderate expression: see section 06 07 15 16
tongue
moderate moderate
regionalmoderate expression: see section 10 11 12 weak expression: see section 09
nucleus pulposus
strong strong
regionalstrong expression: see section 06 09 moderate expression: see section 11 12 13
clavicle
strong strong
regionalstrong expression: see section 08 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6506
Entity Detected:Dusp1, dual specificity phosphatase 1 ( MGI:105120)
Sequence:sense strand is shown

>T6506
CGGGAGGAAAGCGCGGTGAAGCCAGATTAGGAGCAGCGAGCACTTGGGGACTTAGGGCCACAGGACACCG
CACAAGATCGACCGACTTTTTCTGGAGAACCGCAGAACGGGCACGCTGGGGTCGCTGGGGCTGGCCATGG
TGATGGAGGTGGGCATCCTGGACGCCGGGGGGCTGCGCGCGCTGCTGCGAGAGGGCGCCGCGCAGTGCCT
GTTGTTGGATTGTCGCTCCTTCTTCGCTTTCAACGCCGGCCACATCGCGGGCTCAGTGAACGTGCGCTTC
AGCACCATCGTGCGTCGCCGCGCCAAGGGCGCCATGGGCCTGGAGCATATCGTGCCCAACGCTGAACTGC
GTGGCCGCCTGCTGGCCGGAGCCTACCACGCCGTGGTGCTGCTGGACGAGCGCAGCGCCTCCCTGGACGG
CGCCAAGCGCGACGGCACCCTGGCCCTGGCCGCGGGCGCGCTCTGCCGAGAGGCGCGCTCCACTCAAGTC
TTCTTTCTCCAAGGAGGATATGAAGCGTTTTCGGCTTCCTGCCCTGAGCTGTGCAGCAAACAGTCCACCC
CCACGGGGCTCAGCCTCCCCCTGAGTACTAGTGTGCCTGACAGTGCAGAATCCGGATGCAGCTCCTGTAG
TACCCCTCTCTACGATCAGGGGGGCCCAGTGGAGATCCTGTCCTTCCTGTACCTGGGCAGTGCCTATCAC
GCTTCTCGGAAGGATATGCTTGACGCCTTGGGCATCACCGCCTTGATCAACGTCTCAGCCAATTGTCCTA
ACCACTTTGAGGGTCACTACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCAGCTC
CTGGTTCAACGAGGCTATTGACTTCATAGACTCCATCAAGGATGCTGGAGGGAGAGTGTTTGTTCATTGC
CAGGCCGGCATCTCCCGGTCAGCCACCATCTGCCTTGCTTACCTCATGAGGACTAACCGGGTAAAGCTGG
ACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGGAGTATCATCTCCCCGAACTTCAGCTTCATGGGCCAGCT
GCTGCAGTTTGAGTCCCAAGTGCTAGCCCCTCACTGCTCTGCTGAAGCTGGGAGCCCTGCCATGGCTGTC
CTTGACCGGGGCACCTCTACTACCACAGTCTTCAACTTCCCTGTCTCCATCCCCGTCCACCCCACGAACA
GTGCCCTGAACTACCTTAAAAGCCCCATCACCACCTCTCCAAGCTGCTGAAGGGCAAGGGGAGGTGTGGA
GTTTCACTTGCCACCGGGTTGCCACTCCTCCTGTGGGAGGAGCAATGCAATAACTCTGGGAGAGGCTCAT
GGGAGCTGGTCCTTATTTATTTAACACCCCCCCTCACCCCCCAACTCCTCCTGAGTTCCACTGAGTTCCT
AAGCAGTCACAACAATGACTTGACCGCAAGACATTTGCTGAACTCGGCACATTCGGGACCAATATATTGT
GGGTACATCAAGTCCCTCTGACAAAACAGGGCAGAAGAGAAAGGACTCTGTTTGAGGCAGTTTCTTCGCT
TGCCTGTTTTTTTTTCTAGAAACTTCATGCTTGACACACCCACCAGTATTAACCATTCCCGATGACATGC
GCGTATGAGAGTTTTTACCTTTATTTATTTTTGTGTAGGTCGGTGGTCTGCCTTCACAAATGTCATTGTC
TACTCATAGAAGAACCAAATACCTCAATTTTGTGTTTGCGTACTGTACTATCTTGTAAATAGACCCAGAG
CAGGTTTGCTTTCGGCACTGACAGACAAAGCCAGTGTAGGTTTGTAGCTTTCAGTTATCGACAGTTGTAT
GTTTATTTATGATCTGAAGTAATATATTTCTTCTTCTGAGAAGACATTTTGTTACTGGGATGACTTTTTT
TATACAACAGAATAAATTATGACGTTTCTATTGAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3589849 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589849 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19424 same embryo
 EMAGE:19419 same embryo
 EMAGE:19422 same embryo
 EMAGE:19421 same embryo
 EMAGE:19420 same embryo
 EurExpress:euxassay_005013 same experiment
 MGI:4824412 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS