EMAGE mouse gene expression spatial database

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EMAGE:19423

Dusp1 dual specificity phosphatase 1 ( MGI:105120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005013. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005013_01	euxassay_005013_02	euxassay_005013_03	euxassay_005013_04

euxassay_005013_05	euxassay_005013_06	euxassay_005013_07	euxassay_005013_08	euxassay_005013_09

euxassay_005013_10	euxassay_005013_11	euxassay_005013_12	euxassay_005013_13	euxassay_005013_14

euxassay_005013_15	euxassay_005013_16	euxassay_005013_17	euxassay_005013_18	euxassay_005013_19

euxassay_005013_20	euxassay_005013_21	euxassay_005013_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19423_wholemount_strong.wlz

19423_wholemount_moderate.wlz

19423_wholemount_weak.wlz

19423_wholemount_possible.wlz

19423_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19423_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 09 10

forearm mesenchyme

moderate

regional

moderate expression: see section 01 02

foot mesenchyme

moderate

regional

moderate expression: see section 04 05 06

upper leg mesenchyme

moderate

regional

moderate expression: see section 01 02

femur

strong

regional

strong expression: see section 07 moderate expression: see section 21

brain

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 14

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 14 15 16 17 18 19

vagus x ganglion

weak

regional

weak expression: see section 05 13

spinal cord

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12

neural retina

weak

regional

weak expression: see section 01 02 03 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 12 13 14 moderate expression: see section 06 07 15 16

tongue

moderate

regional

moderate expression: see section 10 11 12 weak expression: see section 09

nucleus pulposus

strong

regional

strong expression: see section 06 09 moderate expression: see section 11 12 13

clavicle

strong

regional

strong expression: see section 08 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6506
Entity Detected:	Dusp1, dual specificity phosphatase 1 ( MGI:105120)
Sequence:	sense strand is shown >T6506 CGGGAGGAAAGCGCGGTGAAGCCAGATTAGGAGCAGCGAGCACTTGGGGACTTAGGGCCACAGGACACCG CACAAGATCGACCGACTTTTTCTGGAGAACCGCAGAACGGGCACGCTGGGGTCGCTGGGGCTGGCCATGG TGATGGAGGTGGGCATCCTGGACGCCGGGGGGCTGCGCGCGCTGCTGCGAGAGGGCGCCGCGCAGTGCCT GTTGTTGGATTGTCGCTCCTTCTTCGCTTTCAACGCCGGCCACATCGCGGGCTCAGTGAACGTGCGCTTC AGCACCATCGTGCGTCGCCGCGCCAAGGGCGCCATGGGCCTGGAGCATATCGTGCCCAACGCTGAACTGC GTGGCCGCCTGCTGGCCGGAGCCTACCACGCCGTGGTGCTGCTGGACGAGCGCAGCGCCTCCCTGGACGG CGCCAAGCGCGACGGCACCCTGGCCCTGGCCGCGGGCGCGCTCTGCCGAGAGGCGCGCTCCACTCAAGTC TTCTTTCTCCAAGGAGGATATGAAGCGTTTTCGGCTTCCTGCCCTGAGCTGTGCAGCAAACAGTCCACCC CCACGGGGCTCAGCCTCCCCCTGAGTACTAGTGTGCCTGACAGTGCAGAATCCGGATGCAGCTCCTGTAG TACCCCTCTCTACGATCAGGGGGGCCCAGTGGAGATCCTGTCCTTCCTGTACCTGGGCAGTGCCTATCAC GCTTCTCGGAAGGATATGCTTGACGCCTTGGGCATCACCGCCTTGATCAACGTCTCAGCCAATTGTCCTA ACCACTTTGAGGGTCACTACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCAGCTC CTGGTTCAACGAGGCTATTGACTTCATAGACTCCATCAAGGATGCTGGAGGGAGAGTGTTTGTTCATTGC CAGGCCGGCATCTCCCGGTCAGCCACCATCTGCCTTGCTTACCTCATGAGGACTAACCGGGTAAAGCTGG ACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGGAGTATCATCTCCCCGAACTTCAGCTTCATGGGCCAGCT GCTGCAGTTTGAGTCCCAAGTGCTAGCCCCTCACTGCTCTGCTGAAGCTGGGAGCCCTGCCATGGCTGTC CTTGACCGGGGCACCTCTACTACCACAGTCTTCAACTTCCCTGTCTCCATCCCCGTCCACCCCACGAACA GTGCCCTGAACTACCTTAAAAGCCCCATCACCACCTCTCCAAGCTGCTGAAGGGCAAGGGGAGGTGTGGA GTTTCACTTGCCACCGGGTTGCCACTCCTCCTGTGGGAGGAGCAATGCAATAACTCTGGGAGAGGCTCAT GGGAGCTGGTCCTTATTTATTTAACACCCCCCCTCACCCCCCAACTCCTCCTGAGTTCCACTGAGTTCCT AAGCAGTCACAACAATGACTTGACCGCAAGACATTTGCTGAACTCGGCACATTCGGGACCAATATATTGT GGGTACATCAAGTCCCTCTGACAAAACAGGGCAGAAGAGAAAGGACTCTGTTTGAGGCAGTTTCTTCGCT TGCCTGTTTTTTTTTCTAGAAACTTCATGCTTGACACACCCACCAGTATTAACCATTCCCGATGACATGC GCGTATGAGAGTTTTTACCTTTATTTATTTTTGTGTAGGTCGGTGGTCTGCCTTCACAAATGTCATTGTC TACTCATAGAAGAACCAAATACCTCAATTTTGTGTTTGCGTACTGTACTATCTTGTAAATAGACCCAGAG CAGGTTTGCTTTCGGCACTGACAGACAAAGCCAGTGTAGGTTTGTAGCTTTCAGTTATCGACAGTTGTAT GTTTATTTATGATCTGAAGTAATATATTTCTTCTTCTGAGAAGACATTTTGTTACTGGGATGACTTTTTT TATACAACAGAATAAATTATGACGTTTCTATTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589849 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19424 same embryo
	EMAGE:19419 same embryo
	EMAGE:19422 same embryo
	EMAGE:19421 same embryo
	EMAGE:19420 same embryo
	EurExpress:euxassay_005013 same experiment
	MGI:4824412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source