EMAGE mouse gene expression spatial database

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EMAGE:19466

Mpp3 membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) ( MGI:1328354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005156. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005156_01	euxassay_005156_02	euxassay_005156_03	euxassay_005156_04

euxassay_005156_05	euxassay_005156_06	euxassay_005156_07	euxassay_005156_08	euxassay_005156_09

euxassay_005156_10	euxassay_005156_11	euxassay_005156_12	euxassay_005156_13	euxassay_005156_14

euxassay_005156_15	euxassay_005156_16	euxassay_005156_17	euxassay_005156_18	euxassay_005156_19

euxassay_005156_20	euxassay_005156_21	euxassay_005156_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19466_wholemount_strong.wlz

19466_wholemount_moderate.wlz

19466_wholemount_weak.wlz

19466_wholemount_possible.wlz

19466_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19466_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 09 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 05 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 05 06 07 15 16 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 07 14

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 16 moderate expression: see section 15

cervical ganglion

strong

regional

strong expression: see section 08 16 moderate expression: see section 15

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16 17 18 19

retina

strong

regional

strong expression: see section 01 19 20 21 moderate expression: see section 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 07 08 09 11 12 13 14 moderate expression: see section 04

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 09 10

tongue

weak

regional

weak expression: see section 06 07 08 09 10 11 12

stomach

weak

regional

weak expression: see section 01 02 03 04 06 07 08

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 15 16 17 18

metanephros

moderate

spotted

moderate expression: see section 07 weak expression: see section 17 18

testis

moderate

regional

moderate expression: see section 04 05 06 07 weak expression: see section 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8619
Entity Detected:	Mpp3, membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) ( MGI:1328354)
Sequence:	sense strand is shown >T8619 AGGCTGGTGGCTCCCGCGCGGCTCCGGGTCACCGATCCCAGATCCTGGCGCGACGAGAGGTCATAAGCAG ATCCTGGTGATGTGTGTTCATTACTGAGAGATTCCTCTCCCACCCACACAAACTGTAGGGACAGCTGGGG ACCTGCTGGGCACAGGCATGCCAGTACTGTCTGAAGACTCGGGTTTGCATGAGACCCTGGCACTGCTCAC CTCTCAGCTCAGGCCTGACTCCAACCATAGGGAGGAGATGGGCTTCCTGAGAGATGTCTTCAGTGAGAAG AGCCTCAGTTATTTAATGAAGATTCATGAGAAACTCCGCTATTATGAGAGGCAGAGTCCAACCCCAGTCC TCCACAGCGCCATGGCCCTTGCTGAGGATGTGATGGAGGAGCTGCAGGCTGCCTCTGTGCACAGTGATGA GCGGGAGCTGCTGCAGCTGCTGTCCACCCCCCACCTCAGGGCTGTGCTCATGGTCCACGACACGGTTGCC CAGAAGAACTTCGATCCTGTTCTGCCCCCTCTGCCCGATAACATCGATGAGGATTTTGAAGAGGAGTCGG TGAAGATTGTTCGCTTGGTGAAGAATAAGGAGCCCCTGGGTGCCACCATCCGACGGGATGAGCATTCGGG GGCTGTTGTGGTGGCCAGGATCATGCGAGGGGGTGCGGCAGACCGGAGCGGTCTGGTCCACGTTGGCGAT GAGCTCCGAGAGGTGAATGGGATCGCCGTCCTGCACAAGCGTCCAGACGAGATCAGCCAAATTCTGGCCC AATCCCAGGGATCCATTACCCTGAAGATCATCCCTGCCACCCAGGAGGAAGACCGCTTCAAAGACAGCAA GGTATTCATGCGTGCCCTCTTCCACTATGACCCTCGGGAGGACCGTGCCATCCCCTGCCAGGAGGCTGGC CTGCCCTTCCAGCGGAGGCAGGTCCTGGAGGTGGTGAGCCAAGATGACCCCACCTGGTGGCAGGCCAAGA GAGTAGGCGACACCAACCTTCGAGCTGGCCTCATCCCATCTAAGCAGTTCCAGGAGAGGCGGCTGAGCTA CCGGAGAACCACTGGCACCTTACCAAGCCCCCAGAACTTCAAAAAGCCCCCCTATGATCAGCCTTGTGAC AAAGAGACCTGTGACTGTGATGGGTACTTTAAAGGACATTATGTGGCTGGTCTTCGGAGAAGCTTCCGGC TGGGCTGCAGGGAGAGGCTGGGTGGCTCGCAGGAAGCAAAGGTCCCTACAGGAGCTGAGTCTCAGGTGCT GCTGACGTACGAGGAAGTGGCCAGGTACCAACACCAGCCTGGGGAGCGGCCCCGCCTGGTGGTTCTCATT GGGTCCCTGGGAGCCCACCTCCATGAGCTGAAGCAAAGGGTGGTGGCTGAGGACCCCCAGCAGTTTGCCG TTGCAGTTCCACACACCACCAGGCCCCGGAAGAGCCACGAGAGAGACGGGGTGGAGTACCATTTTGTCTC CAAGCAAGCGTTTGAGGCCGACGTACATCACAACAAGTTCCTGGAGCATGGAGAATATAAAGAGAATCTC TACGGCACCAGCCTAGAGGCCATTCAGGCTGTTATGGCCAAAAACAAAGTGTGTTTGGTGGATGTAGAAC CAGAAGCACTAAGACACCTGAGGACCCCTGAGTTTAAGCCCTATGTTATATTTGTGAAGCCTGCAATTCA GGAGAGAAGGAAGACGCCGCCGGTGTCCCCAGACTCTGAGGACATAGCATCTTCACTTGATGAACAGCAG CAAGAGATGGCCGCCTCTGCTGCCTTCATAGATCAGCACTATGGGCACCTGATAGACACTGTGCTCGTGA GGCAGGACCTGCAGGAGGCCTGCAGCCAGCTCAGAGCTGTCATAGAGACGCTGAGCAAGGACACTTCCTG GGTACCCATCAGCTGGGTGAGGTAACTTCACCCCATAGCATCTGGTCTGAAGGGACCTTGAAGACCCTCG AGTCCAGCCTCCCTCATGTACCATCAAGGAATCTCAAGTGCAGAGAGGAACAGGACTTCCCACGAGCCAT CGCCACCGAGCATCGGTGGGAAGCCTTGCTCAGTGTTGGATTTCTGTCTGCTGTTTGCACTGCAGCCCTT CACGAGCGGAGCTGAAAGGGGCAGTCCACAGAATAGCGTGTTGTTCATTAAAGTCTCGCAGGCAAGTTGG CCTTGATTCTTTGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5718318 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5718318 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19467 same embryo
	EMAGE:19468 same embryo
	EMAGE:19469 same embryo
	EMAGE:19464 same embryo
	EMAGE:19465 same embryo
	EurExpress:euxassay_005156 same experiment
	MGI:4826431 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source