EMAGE mouse gene expression spatial database

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EMAGE:19498

Tnfaip8 tumor necrosis factor, alpha-induced protein 8 ( MGI:2147191)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009284. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009284_01	euxassay_009284_02	euxassay_009284_03	euxassay_009284_04

euxassay_009284_05	euxassay_009284_06	euxassay_009284_07	euxassay_009284_08	euxassay_009284_09

euxassay_009284_10	euxassay_009284_11	euxassay_009284_12	euxassay_009284_13	euxassay_009284_14

euxassay_009284_16	euxassay_009284_17	euxassay_009284_18	euxassay_009284_19	euxassay_009284_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19498_wholemount_strong.wlz

19498_wholemount_moderate.wlz

19498_wholemount_weak.wlz

19498_wholemount_possible.wlz

19498_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19498_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 11 weak expression: see section 09 10 12 13

thymus primordium

moderate

regional

moderate expression: see section 08 09 10 weak expression: see section 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 weak expression: see section 06 14 15 16

hypothalamus ventricular layer

weak

regional

weak expression: see section 12 13 14 15

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 12 13 14 15

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 08 09 10 11 17 weak expression: see section 12 15 16

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 11 12 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 weak expression: see section 03 04 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

weak

regional

weak expression: see section 07 08 09 11 12 13 14 15 16

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 16

nasal septum

moderate

regional

moderate expression: see section 11 12 14 15 weak expression: see section 13

viscerocranium

moderate

regional

Expression in the turbinate bone.

cricoid cartilage

moderate

regional

moderate expression: see section 11 12

thyroid cartilage

moderate

regional

moderate expression: see section 08 09 11 12

trachea

moderate

regional

moderate expression: see section 11 weak expression: see section 10

axial skeleton

weak

regional

weak expression: see section 08 09 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6867
Entity Detected:	Tnfaip8, tumor necrosis factor, alpha-induced protein 8 ( MGI:2147191)
Sequence:	sense strand is shown >T6867 GTCTAGGAGCCTCGCAGCCTCACGTCCATGTCCGTGGCGGTGGCGCCTGTGGCTGTGCACCCCGACAGCA TGCTCTCCGAAGCGGAGGAGCCCAGGGAAGTGGCTACAGATGTCTTCAATTCCAAAAACCTGGCCGTTCA GGCACAAAAGAAGATCCTGGGCAAAATGGTATCCAAATCCATCGCCACCACGCTGATCGACGACACCAGC AGCGAGGTGCTAGATGAGCTGTACAGGGTGACCAAGGAGTACACCCAGAACAAGAAGGAGGCGGAGAGGG TCATCAAGAACCTCATCAAGACGGTCATCAAGCTGGCCGTCCTCCACAGGAACAATCAGTTCAATCAAGA CGAGCTGGCGCTCATGGAGAAGTTCAAGAAGAAGGTGCACCAGCTTGCCATGACGGTCGTCAGCTTCCAC CAGGTAGAGTACACCTTCGACCGCAATGTGCTGTCCAGGCTGCTGAACGAGTGCCGAGAGCTCCTACACG AGATCATTCAGCGCCACCTTACCGCCAAGTCTCACGGACGGGTTAATAATGTCTTTGACCATTTTTCAGA TTGTGATTTTTTGGCTGCCTTGTACAATCCCTTTGGAAAGTTTAAACCTCACTTACAGAAACTTTGCGAC GGCATCAACAAAATGTTGGATGAAGAGAACATATGAGCTTGCGAAGTAAGATTGTGACCAGCAGCGAGTT AGGTGAAGTCCAGCACCGATGACTTGTAAAGGGAAGACGGAGACTCTTTTATCAATCACATGTATTTCAG AAAGGCTTGGCCCCCATATGACCTGTTATTGTTGTTGGACATTAATAGTAGTACTTCTGTGTGGCTTGTT GTATTTGAAAGGAAAAAAAAAAGTGTATTGCCAAAAATTCTGGCTGCAAACGTCTTAATGATTGGCAAGA TGTGAGAAAAACGGATAGTTGGTCATTCAGATGTCAAGGGATACAAAGACAGATGAGTGTGGCCCCAAGC GCTGGCACTTCTTGTGTTTTAGGGAAATCATGTTGGTGGCACGTTGGATATTTCTAATATGTATAAAGCC GTGTATCTTGACTCACCTTGATCCTTTGCTACGTCTGTGTATCTCTCTTACAATGTCGAGACCCTCTCTT TGCTGTCATTGATCCTTTTGAAACAACTCTGCTTCTTTGGTTACAGATCGTCATTGACCTTCAGGAGTTA AATTTGAGGCAGTCAAACCGAATGCTCAGGAAAGCAAAAAAAACTGTGCATCTGAAATCATGACTGTGGG AGTGAACAATCTTAGACATCATGAGCAGTGTCCATTGCAAGAGTGTGTTTTTGAAGCTGACTTCTACCTT TTAGTACTATCAGTGATACTTAGAGTCTCAGAGCTAGTGGCATGATATTGCCTTGAAATATCTGCTTAGG GCATAACTTTGATGTCATCTTGAACATGTACTGACATGTTCCCGTATTAACATCTATTTCACCTGTAGGA AGAATAAATCTCTAGATTGGCTTTGATATTGAGACAATAAAATGTAAGTAGCGTTTCAAAAAGAAAAACT TCTTGAATTTAAAGATGAATTCATCAGACTGATTTATGGGTGAAAGGATAGAAAGGACGTTGCTAAGTAA CATAAGAAAGATGCATGTATTTCAGTGTGTAACAGGGATTGATGAATAAAAGATGCCATTTTTTATTTAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3965693 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3965693 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19495 same embryo
	EMAGE:19494 same embryo
	EMAGE:19497 same embryo
	EMAGE:19493 same embryo
	EMAGE:19496 same embryo
	EurExpress:euxassay_009284 same experiment
	MGI:4828841 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source