EMAGE mouse gene expression spatial database

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EMAGE:19568

Tbce tubulin-specific chaperone E ( MGI:1917680)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004971. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004971_01	euxassay_004971_02	euxassay_004971_03	euxassay_004971_04

euxassay_004971_05	euxassay_004971_06	euxassay_004971_07	euxassay_004971_08	euxassay_004971_09

euxassay_004971_10	euxassay_004971_11	euxassay_004971_12	euxassay_004971_13	euxassay_004971_14

euxassay_004971_15	euxassay_004971_16	euxassay_004971_17	euxassay_004971_18	euxassay_004971_19

euxassay_004971_20	euxassay_004971_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19568_wholemount_strong.wlz

19568_wholemount_moderate.wlz

19568_wholemount_weak.wlz

19568_wholemount_possible.wlz

19568_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19568_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 03 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15 not examined expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 15 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 14

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 12

cervical ganglion

moderate

regional

moderate expression: see section 07 13 14

thoracic ganglion

moderate

regional

moderate expression: see section 11

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 12 13 14 15

retina

moderate

regional

moderate expression: see section 01 02 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14 15

liver

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

metanephros

weak

regional

weak expression: see section 05 06 07 08 09 13 14 15 16

urethra of male

moderate

regional

moderate expression: see section 09 weak expression: see section 10

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8007
Entity Detected:	Tbce, tubulin-specific chaperone E ( MGI:1917680)
Sequence:	sense strand is shown >T8007 GCCGAAAGCTTCGTCGCTGATTGCGTGAGGGTCTCTGGGTGCTGACAGACCGGCAGAGGGAAGATCTCCT ATTGTGGATTCCTGCTATCTTATAATGAGTGACATTTTGCCATTAGATGTCATTGGTAGAAGAGTTGAAG TTAATGGAGAATATGCAACAGTGCGCTTTTGTGGTGCTGTTCCTCCTGTGGCAGGACTCTGGTTAGGAGT TGAGTGGGACAATCCCGAGAGAGGAAAGCATGACGGGAGCCACGAAGGTACTATGTATTTTAAATGCAGG CACCCAACAGGAGGCTCTTTTGTTCGTCCAAGCAAAGTTAATTTCGGAGATGACTTCCTTACTGCCCTTA AGAAGCGCTATGTGCTCGAAGATGGACCTGATGATGATGAAAATTCGTGTTCCTTAAAAGTTGGAAGTAA ACAAGTGCAAACTATTGGTTTTGAACATATTACAAAGAAGCAGAGTCAGCTGAGAGCACTGCAGGACATT TCCCTCTGGAACTGTGCCGTGAGCCATGCTGGTGAACAAGGAAGAATTGCTGAAGCCTGCCCCAATATTC GAGTTGTAAATTTATCCAAAAACTTGTTGTCAACTTGGGATGAAGTGGTTCTTATTGCAGAGCAGCTCAA AGACCTGGAGGCTCTTGATCTCAGTGAAAATAAACTGCAGTTTCCCTCTGACTCACCAACTCTAACTAGA ACATTTTCTACTCTGAAGACTTTAGTCCTAAACAAAACAGGAATAACGTGGACTGAGGTGCTGCACTGTG CTCCTTCATGGCCAGTCCTCGAGGAACTTTACCTCAAGTCTAACAATATTTCCATTTCTGAAAGGCCAGT GAATGTCCTGCAGAAAATGAGGTTACTAGACCTTTCCTCCAACCCATCAATTGATGAAAGCCAGCTGAGC CTTATAGCAGATCTTCCAAGATTAGAACACCTAGTCCTTTCTGACATTGGACTTTCTTCTATACATTTTC CAGATGCTGAAATTGGATGCAAAACATCCATGTTCCCGGCCTTGAAGTACCTCATAGTCAATGACAACCA GATATCAGAATGGTCATTTATCAACGAGCTGGATAAACTGCAGAGCCTGCAGGCCCTGTCCTGCACTAGA AACCCTTTGAGCAAAGCGGACAAAGCAGAGGAGATCATTATCGCCAAGATTGCTCAGCTGAGGACCCTGA ACAGGTGCCAGATTCTTCCAGAGGAAAGGCGTGGAGCTGAGCTAGATTATCGAAAAGCCTTTGGAAATGA ATGGAGAAAGGCTGGTGGACACCCAGATCCAGACAAAAACAGGCCAAATGCAGCGTTTCTCTCAGCCCAT CCTAGATACCAGCTTCTCTGCTGCAAATATGGTGCACCTGAAGATGAGGAGCTCAAAACACAGCAGCCAT TCATGCTAAAAAAACAGCTACTAACCCTGAAGATAAAATGCTCAAATCAACCTGAACGGCAAATATTAGA AAAGCAGTTGCCAGACTCCATGACAGTTCAAAAGGTAAAGGGACTGTTGTCACGTCTTCTCAAAGTTCCT GTGTCAGAACTTCTGTTGTCCTACGAAAGTTCCAAGATGCCGGGCAGAGAAATTGAACTAGAAAATGACC TACAGCCATTACAGTTTTATTCTGTGGAAAATGGAGATTGCCTACTAGTGCGATGGTAACAGTCCAACCG TAAGAGTTTAACGACTGGAATGTATACACCAGAAACAAAACCTTTGCCCTCACAAATGATGTTATGTTTT GTTGGGAAAAGACCATTTGGTTGTATATTAATAGAATAATAAAGGCTTTGATTACTAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30009463 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30009463 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19571 same embryo
	EMAGE:19567 same embryo
	EMAGE:19570 same embryo
	EMAGE:19569 same embryo
	EMAGE:19572 same embryo
	EurExpress:euxassay_004971 same experiment
	MGI:4828613 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source