EMAGE mouse gene expression spatial database

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EMAGE:19606

Wnk4 WNK lysine deficient protein kinase 4 ( MGI:1917097)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005023. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005023_01	euxassay_005023_02	euxassay_005023_03	euxassay_005023_04

euxassay_005023_05	euxassay_005023_06	euxassay_005023_07	euxassay_005023_08	euxassay_005023_09

euxassay_005023_10	euxassay_005023_11	euxassay_005023_12	euxassay_005023_13	euxassay_005023_14

euxassay_005023_15	euxassay_005023_16	euxassay_005023_17	euxassay_005023_18	euxassay_005023_19

euxassay_005023_20	euxassay_005023_21	euxassay_005023_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19606_wholemount_strong.wlz

19606_wholemount_moderate.wlz

19606_wholemount_weak.wlz

19606_wholemount_possible.wlz

19606_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19606_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 04 05 weak expression: see section 20

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 04 05 weak expression: see section 20

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 04 05 weak expression: see section 20

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 04 05 18 weak expression: see section 20

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 04 05 18 19

otic capsule

weak

regional

weak expression: see section 04 05 06 07 14 15 16 17 18 19

nasal septum

moderate

regional

moderate expression: see section 13 14 15 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

heart atrium

strong

regional

strong expression: see section 11 12 moderate expression: see section 09 10

stomach

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 08 09

midgut loop

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 12 13 15

upper jaw incisor

strong

regional

strong expression: see section 13

renal cortex

moderate

regional

moderate expression: see section 03 04 05 06 07 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7439
Entity Detected:	Wnk4, WNK lysine deficient protein kinase 4 ( MGI:1917097)
Sequence:	sense strand is shown >T7439 GCCTGTCAGGCAGCAACCTCCAGCCTCCTGGCTTTCCGTTCTGCGGCGCCTGGCCCTTCCCATGCTAGCA CCTCGAAATACGGAGACTGGGGTCCCCATGTCCCAAACCGAGGCTGACCTGGCTCTGCGGCCATCTCCGG CTCTCACCTCTACGGGGCCGACCCGCCTGGGGCCACCTCCTCGCCGAGTGCGCCGCTTCTCTGGGAAGGC TGAGCCTCGGCCGCGCTCTTCGAGACCTAGCCGCCGCAGCTCAGTCGATCTGGGACTGCTGAGCTCCTGG TCTCAACCAGCCTCACTCCTTCCGGAACCCCCGGATCCTCCAGACTCCGCTGGCCCCACGAGGAGCCCAC CTTCAAGCTCTAAAGAACCCCCCGAGGGCACATGGATGGGGGCAGCTCCCGTGAAGGCTGTGGACTCTGC ATGTCCTGAGCTTACGGGATCTTCAGGAGGTCCAGGGTCCCGGGAGCCACCGAGGGTTCCTGATGCTGCA GCCCGGGAACGGCGGCGGGAGCAGGAAGAAAAAGAGGACACAGAAACCCAGGCTGTGGCAACGTCTCCGG ACGGCCGATACCTCAAGTTTGACATTGAGATTGGAAGAGGTTCCTTCAAGACCGTGTATCGAGGGCTAGA CACCGACACCACGGTAGAGGTGGCCTGGTGTGAGCTGCAGACCCGGAAACTCTCTCGGGCCGAGCGGCAG CGTTTCTCCGAGGAAGTTGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCTATGACTCCT GGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACAGAACTCATGACCTCGGGCACGCTCAA GACGTACCTGAGGCGGTTCCGCGAAATGAAGCCCCGAGTCCTTCAGCGCTGGAGTCGCCAGATTCTACGG GGACTTCATTTCCTTCATTCCCGAGTGCCCCCCATCCTGCACCGAGATCTCAAGTGTGACAACGTCTTCA TTACTGGCCCTTCAGGCTCTGTCAAAATCGGAGACCTCGGACTGGCCACGCTCAAGCGCGCCTCCTTTGC CAAGAGCGTCATTGGGACCCCGGAATTCATGGCTCCTGAGATGTACGAGGAAAAGTACGACGAGGCTGTG GATGTGTACGCCTTTGGCATGTGCATGCTGGAGATGGCTACGTCTGAGTATCCCTACTCCGAGTGTCAGA ATGCAGCACAAATCTACCGCAAGGTCACTTCGGGCACAAAGCCCAACAGCTTCTACAAGGTGAAGATGCC AGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGGTTCACCATCCAGGATCTC CTGGCCCATGCGTTCTTCCGCGAGGAACGAGGTGTGCACGTGGAGCTGGCAGAGGAAGATGATGGAGAGA AGCCGGGCCTCAAGCTCTGGCTGCGCATGGAGGATGCGCGGCGCGGGGGGCGTCCACGAGACAACCAGGC CATCGAGTTCCTGTTTCAGCTCGGCCGGGACGCTGCGGAGGAGGTGGCTCAGGAGATGGTAGCCTTGGGC TTAGTCTGTGAAGCTGACTACCAGCCAGTGGCCCGTGCAGTTCGAGAACGGGTTGCTGCTATCCAGCGGA AGCGGGAGAAGCTTCGAAAAGCTAGGGAATTGGAGGTTCTCCCACCAGACTCAGGACCTCCTCCAGCAAC TGTGTCTCTGGCTCCGGGTCCCCCCAGTGCCTTCCCCCCAGAGCCTGAGGAGCCAGAGGCTGACCAGCAT CAATCCTTCCTCTTCCGCCATGCCAGCTACTCATCAACTACATCTGATTGCGAGACTGATGGCTACCTCA GTTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCTGGGGGGTTACCATCCAGCCCCGC TGAATCCCATCTCTGCTTGCCCTCGGGTTTTGCCTTGTCTATTCCACGGTCTGGCCCTGGCAGTGACTTT TCTCCTGGGGATAGCTACGCCTCAGATGCGGCATCAGGCCTTAGTGACATGGGAGAAGGGGGGCAAATGA GGAAAAATCCAGTGAAGACTCTTCGACGGAGACCTCGATCCCGGCTCCGGGTCACTAGTGTCTCAGACCA GAGCGACAGAGTTGTCGAGTGTCAGCTGCAGACTCACAACAGCAAGATGGTGACGTTCCGATTTGATCTG GATGGGGACAGCCCAGAAGAAATTGCAGCTGCCATGGTATACAATGAGTTCATCCTGCCCTCGGAGCGAG ATGGATTCCTGAGCCGGATCCGGGAGATTATCCAGCGAGTGGAGACCCTGCTGAAGAGAGATGCTGGTCC CCCAGAGGCTGCTGAAGATGCCCTGAGCCCCCAGGAAGAGCCAGCAGCCCTGCCTGCCCTCCCAGGCCCA CCCAATGCAGAGCCCCAGAGAAGCATCTCCCCAGAACAGAGGAGCTGGGCAGCCTTCTCCACCTCTCCAT CTTCTCCTGGCACCCCCTTGTCCCCTGGGGCCCCCTTTTCCCCTGGGACCCCTCCTGTCTTCCCATGCCC CATCTTTCCTATCACTTCACCCTCATGCTATCCCTGCCCATTCTCCCAGGTCTCTTCAAACCCCTATCCA CAGGCCCCCAGTTCCCTGCTTCCCTTATCCTCCAGTGCTTCTCAGGTTCCACTTCCATCTTCCTCTCTTC CCATCAGCGCTCCCCTCCCATTCTCTCCTAGTTATCCCCAAGACCCTCTTAGCCCCACTTCTCTTCCCGT CTGCCCCTCTCCTCCCTCTCTTCCCTCTACCACAGCAGCCCCTCTCCTCTCTCTGGCTAGTGCCTTCTCT CTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCATCCCCTGGACTTCTTTCTCAGTCTCCTCCAG CCCCTCCAGGTCCTCTGCCTAGCCTGCCCCTTTCCCTTGCTTCTTGTGACCAGGAGAGCCTTTCGGCCCA AACAGCTGAGACAGAGAATGAGGCTTCCCGTAATCCTGCTCAGCCACTACTGGGTGATGCTAGACTGGCA CCTATATCTGAAGAGGGAAAGCCCCAGCTTGTTGGCCGTTTCCAAGTGACTTCATCTAAGGAACCAGCAG AGCCTCCCCTGCAACCAGCATCCCCAACTCTCTCCAGATCCCTAAAGCTGCCAAGCCCTCCGCTGACCTC AGAGAGCTCAGACACAGAGGACAGTGCTGCAGGAGGCCCAGAGACTAGGGAGGCTCTGGCAGAGAGTGAC CGTGCAGCCGAAGGCCTGGGGGTTGCGGTCGATGATGAAAAGGATGAAGGGAAGGAACCCCTACTTGGAG GCAGTTCCCCAATCTTGAGCCATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCTGTGTCTGAG CAGTGAGGAGTCGGAGAGCAGCGGGGAAGACGAGGAATTCTGGGCTGAGCTGCAGAACCTTCGGCAGAAG CACTTGTCGGAAGTGGAGGCACTACAGACACTACAGAAGAAGGAAATCGAGGACTTATACAGCCGGCTTG GGAAACAGCCCCCACCTGGTATTGTAGCTCCAGCTGCTATGCTGTCCTGCCGCCAGCGCCGCCTCTCGAA GGGCAGCTTTCCCACCTCCCGCCGCAACAGCCTGCAGCGCTCTGATCTCCCTGGTCCTGGCATCATGCGA AGGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGACATTCGCCG GGGATATTGGCAGGATGTGAATTCAGACCAGAAGTCCTATGTCTCCCCAACACCAGGGCCCCACCATGGC CCATGGCCTCTCAGAATCTGATGTTTTTTGTGACCAACTCAACTCAGCAAAGAACACCCCAGCACTGAGT GGGTAGAAGGCCCGTGGGCACGGAGAGTGCGTTGTGTTGTATCCTGAAGAGTCAAAACCTTGCGGGTTCT CTTTGCTGTGTGGAAATCTCATTTCTGTAGCCTACCCCACCTGCCCTCCCCAGGCAAGGGCTGACCACTA AGCAATCCGAGTCCAGATGCTGCGGAAGGCCAACTTCCAGCTGGGAGAGTGGAGGGGCTGTGCTCCTGCC ACCAAAGTCAACAGCAACCAATAAAAATACTGAGGAGAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4234311 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4234311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19607 same embryo
	EurExpress:euxassay_005023 same experiment
	MGI:4829222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source