EMAGE mouse gene expression spatial database

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EMAGE:19608

Pgam2 phosphoglycerate mutase 2 ( MGI:1933118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005053. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005053_01	euxassay_005053_02	euxassay_005053_03	euxassay_005053_04

euxassay_005053_05	euxassay_005053_06	euxassay_005053_07	euxassay_005053_08	euxassay_005053_09

euxassay_005053_10	euxassay_005053_11	euxassay_005053_12	euxassay_005053_13	euxassay_005053_14

euxassay_005053_15	euxassay_005053_16	euxassay_005053_17	euxassay_005053_18	euxassay_005053_19

euxassay_005053_20	euxassay_005053_21	euxassay_005053_22	euxassay_005053_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19608_wholemount_strong.wlz

19608_wholemount_moderate.wlz

19608_wholemount_weak.wlz

19608_wholemount_possible.wlz

19608_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19608_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 13 14 15 16 17 18 19 20 21 moderate expression: see section 03 04 12

forearm mesenchyme

strong

regional

strong expression: see section 19 20 21 22

shoulder mesenchyme

strong

regional

strong expression: see section 19 20 21 22

upper arm mesenchyme

strong

regional

strong expression: see section 19 20 21 22

foot mesenchyme

strong

regional

strong expression: see section 01 17 18

lower leg mesenchyme

strong

regional

strong expression: see section 01 18 19 20 21 22

upper leg mesenchyme

strong

regional

strong expression: see section 01 18 19 20

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thymus primordium

strong

regional

strong expression: see section 06 07 08 09 10

hypothalamus ventricular layer

weak

regional

weak expression: see section 10 11 12 13 14

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12 13 14

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 14 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 10 11 12 13 14 15 16 17

heart

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15

tongue muscle

strong

regional

strong expression: see section 08 09 10 11 12

tail mesenchyme

strong

regional

strong expression: see section 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7456
Entity Detected:	Pgam2, phosphoglycerate mutase 2 ( MGI:1933118)
Sequence:	sense strand is shown >T7456 GTCCCCCGCCACCATGACCACCCACCGCCTAGTAATGGTTCGCCACGGTGAGAGCTTATGGAACCAAGAG AACCGTTTCTGTGGCTGGTTTGATGCAGAGCTGAGTGAGAAGGGAGCAGAGGAGGCCAAGCGGGGGGCCA CCGCTATCAAAGATGCCAAGATAGAGTTTGACATCTGCTACACGTCGGTGCTGAAGCGGGCAATCCGCAC CCTTTGGACCATCCTGGATGTTACGGACCAAATGTGGGTGCCCGTGGTGCGTACCTGGCGCCTCAATGAG CGGCACTATGGTGGCCTCACAGGCCTCAATAAGGCTGAGACGGCTGCAAAGCACGGGGAGGAGCAGGTGA AGATCTGGAGGCGTTCCTTTGACACCCCACCACCACCCATGGATGAGAAACACAACTACTACACCTCCAT CAGCAAGGACCGCCGCTATGCAGGCTTGAAGCCTGAGGAGCTGCCTACCTGTGAAAGTCTCAAGGACACC ATTGCCCGGGCCTTGCCCTTCTGGAATGAGGAGATCGCACCTAAGATTAAGGCTGGCCAGAGAGTGCTTA TTGCAGCCCATGGGAACAGCCTTCGGGGCATTGTGAAACATCTGGAAGGGATGTCCGACCAGGCCATCAT GGAGCTGAACCTGCCCACTGGAATCCCCATTGTGTATGAGCTGGACCAGAACCTGAAGCCCACCAAGCCC ATGAGGTTCCTGGGAGACGAAGAGACGGTTCGGAAGGCCATGGAAGCTGTTGCTGCCCAGGGAAAGGCGA AGTGAGGGTGGACGGGGCAATAAAGTTACTTCCGCACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235174 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235174 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19613 same embryo
	EMAGE:19609 same embryo
	EMAGE:19611 same embryo
	EMAGE:19610 same embryo
	EMAGE:19612 same embryo
	EurExpress:euxassay_005053 same experiment
	MGI:4827169 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source