EMAGE mouse gene expression spatial database

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EMAGE:19612

Wrb tryptophan rich basic protein ( MGI:2136882)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005059. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005059_01	euxassay_005059_02	euxassay_005059_03	euxassay_005059_04

euxassay_005059_05	euxassay_005059_06	euxassay_005059_07	euxassay_005059_08	euxassay_005059_09

euxassay_005059_10	euxassay_005059_11	euxassay_005059_12	euxassay_005059_13	euxassay_005059_14

euxassay_005059_15	euxassay_005059_16	euxassay_005059_17	euxassay_005059_18	euxassay_005059_19

euxassay_005059_20	euxassay_005059_21	euxassay_005059_22	euxassay_005059_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19612_wholemount_strong.wlz

19612_wholemount_moderate.wlz

19612_wholemount_weak.wlz

19612_wholemount_possible.wlz

19612_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19612_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22 23

facial vii ganglion

strong

regional

strong expression: see section 03 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 15

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 16 17 18 19 20 moderate expression: see section 01 15

vagus x ganglion

strong

regional

strong expression: see section 05 14 moderate expression: see section 13

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 15 16

trigeminal v nerve

moderate

regional

moderate expression: see section 08 13 14

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 10 11 12 13 14 moderate expression: see section 03

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

hindgut

moderate

regional

moderate expression: see section 08 09

rectum

moderate

regional

moderate expression: see section 08 09

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11

midgut loop

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09

mandible

strong

regional

strong expression: see section 01 04 05 06 07 13 14 15 16 17 18 19 moderate expression: see section 02 03

lower jaw incisor

strong

regional

strong expression: see section 08 10 11 12

maxilla

strong

regional

strong expression: see section 04 05 06 07 13 14 15 16 17 18 moderate expression: see section 03

upper jaw incisor

strong

regional

strong expression: see section 08 12

bladder

moderate

regional

moderate expression: see section 06 07 08 09 10

orbito-sphenoid

strong

regional

strong expression: see section 03 20 moderate expression: see section 01 02 19 21 22

clavicle

moderate

regional

moderate expression: see section 05 06 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7458
Entity Detected:	Wrb, tryptophan rich basic protein ( MGI:2136882)
Sequence:	sense strand is shown >T7458 GCGCGGAGGCGCGGTCACCATGGCGCTGTCGTAGCGGTGCAGGTGCCTGGGATCCAGCATGAGCGCGTCC GAGACGGACCGCTGGGCGTGGCTGCTGGTGTTGAGCTTCGTGTTCGGGTGTAACTTGCTGCGCATCCTCC TGCCGTCCCTGTCCTCGTTTATTTCCAGGGTGCTGCAGAAGGATGCAGAGCAGGAGTCACAGATGAGGGC AGAGATCCAGGGCATGAAGCAGGAGCTGTCCACCGTCAACATGATGGACGAGTTTGCCAGATACGCCAGG CTGGAAAGGAAGATCAACAAGATGACGGACAAGCTCAAAACGCACGTGAAGGCACGGACAGCTCAGTTGG CCAAGATCAAGTGGTTTATAAGTGTTGCTTTCTACATACTACAAGCAGCGCTGATGATCTCGCTCATTTG GAAGTATTACTCCGTCCCTGTGGCTGTGGTGCCCAGCAAGTGGATCACCCCACTAGACCGCCTGGTAGCC TTTCCTACTCGAGTAGCAGGTGGAATTGGAATCACCTGTTGGATTTTAGTCTGTAACAAAGTTGTGGCTA TCGTTCTCCACCCTTTCAGCTGAGGGGAAGGCCAGCTACAAGTCTTTACAAAAGGATTCTGATAGGGTAA AGAGCGGATTGCACACAAGTCCACAGATTCTTGTGTTTTATGTTTTGGAACATTTGCTTTTGTGTGTCAC CTGGGATTCTTGTTGAAGTCAGGATTTTCTACACCCGCCATTGAGCCAGAAAGACCAGTTACGGCACTCC CATATCGTCTGATGTAGGTCTGGATTCATACCAACTGGGAACCACCACGTGGCATCTGCTGATGCTTGTT TCTGGGCATGGCTTGGGTCTGTTGCCAGCTGCAGAGTCAGACTTGTAACAACTCCGTTAGTGCTGTTTTA ATTTTAGAAAAACCCACACTTTCGTACCACTACTAAACCTCCTTTTTATCTCAGTTACAGAAAGTGACGC ACACTAGTGATGTAGCTGTACTTGTCTCCTGAAATGTCACACATAAGTGTGTGGAAAGTATCGCTGTATG TCTAAGTTTGTTCATTTTTTATAGTGCTTCCTACATGTGTTCATGAGACGACGTGTATTTTTATTTAAAG CATTTCAAATGAAGTCTGTCTTTAAAGCATTGGGTGTTTATGCTGTGCGCAGAATAGGGTTAAACAAGGT GCGGTCTGATCCAGTTCATCTGAGACTCCTTAGGCTCATGAAGTTACTAGGCAGAAATCTGCCAAGTCTG TTTTTTTTTGTTTGTTTTGTTTTTTTGTTTTTTTTTTTTGTTTTGAGACAAGGTCTCTCAGTGTAGCTGC AGCTCTCCTGAAATTATGTAGACCAGGCTTGTCTCAAACCCACAGAGATCCACCTCCCTCTATCTCCCAG ATGATAGGATTAAAGGCGTATGCCGCTACTGTTTTTGTATATGCCGTCAAGACTGTTTTTGAAGCACTTT CCCTAGTTACAGTATTTGCCAGTGCTAGTGTGACTGTCCCACCAGCCTTACAGCAGGAGCTGGGCTCACT GCCTGCGCTGCAAGCTGCGGTATTCGCCCCTGGGGAACCATAGCACAGAAAAGCTCCCTGACAGCACTGC AGGTCTCTGCACAGCTCTGGCTGTCCTGGAATCACTATGTAGACCAGGCTGACCTGGAATTTATAGGAAT CCTCTCGTCTCTGCCTCATAGCTGCTGGGATTAAAGGCGTAGCACTGGGTTTGAAGGATCATATTTATAC TCCAGTGTTTCCTAATGATCTCCTAAGTAGCTCTTTGTTTTCCTGACTGTACTGTGAACAGGCCCAGTGT GCGGCCCTTGGATTGGTTGTAGACCGAGTGCCACCAGAGCAGGCTTGAGAGTCCATTGATTTAAACTCCG CAGATGCTGCAGATAGATCCACGTGGCACCAAGATTACTTGGCTGTATTTGTTGTGGGCACAAACGGAAC ATAGCAGTAGGCATAGCCAACATTGCCAGGAGGACCTGCCCACTAGCTCCAGCATGCTCATCACGAAGGA AGCACGTGGTCTACACAAGATTATTTTGGGACCTGAGGAGGTTGGCTGCAGAATCTGGGTTCCCTCTGGG TATTCAGCCCTCGGGGTGAGGGAGGGTGTGCGCTTTCCTCTTTTTTGCAGTGAGCCATAGTCTGCAATGG CGCTTTTTGCCCACATCGTTTAGGTAGCCTGGGTCAGCATCCCAAGGCCACTTGGAAGGGCAATCAGAAG TGCCCAGAACAGATGCCTCTCATGATTTCAGCTGACCTGAAGGAACAAATGACTTAGTGTTCCCATGTGT GTAATGTGGATGTCAGCGCTTCCACATGCATAATGTATGTAAGACTGTTAGTGTTCCCATGTGTGTAGTG TATGTAACACCGCAAGTAGGGGACAGCTGTGTTTTTATCCCCAAATTGTATTTGTTTATTAATGGGTAAT AAACATTGAGTTAAAAAGGGACTGGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235618 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235618 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19613 same embryo
	EMAGE:19609 same embryo
	EMAGE:19611 same embryo
	EMAGE:19608 same embryo
	EMAGE:19610 same embryo
	EurExpress:euxassay_005059 same experiment
	MGI:4829228 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source