Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19620

Htra1 HtrA serine peptidase 1 ( MGI:1929076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620
"Pseudo-wholemount" of euxassay_005061. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005061_01 euxassay_005061_02 euxassay_005061_03 euxassay_005061_04
EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620
euxassay_005061_05 euxassay_005061_06 euxassay_005061_07 euxassay_005061_08 euxassay_005061_09
EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620
euxassay_005061_10 euxassay_005061_11 euxassay_005061_12 euxassay_005061_13 euxassay_005061_14
EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620 EMAGE:19620
euxassay_005061_15 euxassay_005061_16 euxassay_005061_17 euxassay_005061_18 euxassay_005061_19
EMAGE:19620 EMAGE:19620
euxassay_005061_20 euxassay_005061_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19620Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19620_wholemount_strong.wlz
19620_wholemount_moderate.wlz
19620_wholemount_weak.wlz
19620_wholemount_possible.wlz
19620_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19620_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diaphragm
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 weak expression: see section 13
elbow rest of mesenchyme
strong strong
regionalstrong expression: see section 01 20
forearm rest of mesenchyme
strong strong
regionalstrong expression: see section 01 02 19 20 21
shoulder rest of mesenchyme
strong strong
regionalstrong expression: see section 01 20
humerus
strong strong
regionalstrong expression: see section 02 03 04 05 16 17 18 19
hand mesenchyme
strong strong
regionalstrong expression: see section 18
hindlimb digit 1 metatarsal
moderate moderate
regionalmoderate expression: see section 05
hindlimb digit 2 metatarsal
moderate moderate
regionalmoderate expression: see section 05 15 16
hindlimb digit 3 metatarsal
strong strong
regionalstrong expression: see section 04 moderate expression: see section 05 15 16
hindlimb digit 4 metatarsal
moderate moderate
regionalmoderate expression: see section 05 15 16
hindlimb digit 5 metatarsal
moderate moderate
regionalmoderate expression: see section 05 15
foot mesenchyme
strong strong
regionalstrong expression: see section 03 moderate expression: see section 16 17
knee rest of mesenchyme
strong strong
regionalstrong expression: see section 01 19 20 21
lower leg rest of mesenchyme
strong strong
regionalstrong expression: see section 02 18 19
upper leg rest of mesenchyme
strong strong
regionalstrong expression: see section 03 18
anterior abdominal wall musculature
strong strong
regionalstrong expression: see section 08 14 moderate expression: see section 01 02 03 04 17 18
axial musculature
strong strong
regionalstrong expression: see section 04 05 14 15 moderate expression: see section 03 16 17 19
pancreas
weak weak
regionalweak expression: see section 05 06 07
vibrissa
moderate moderate
regionalmoderate expression: see section 04 18 weak expression: see section 03 05 19
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11
diencephalon roof plate
strong strong
regionalstrong expression: see section 10 weak expression: see section 11
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17 moderate expression: see section 03 weak expression: see section 11
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 11 12 13 14 15 16 17
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 19 20 21
aorta
strong strong
regionalstrong expression: see section 08 09 10 11 12
tongue
weak weak
regionalweak expression: see section 09 13 14
tongue muscle
weak weak
regionalweak expression: see section 10 11 12
lower jaw incisor
weak weak
regionalweak expression: see section 10 13
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 weak expression: see section 06 16 17
upper jaw incisor
weak weak
regionalweak expression: see section 09 10 13
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 weak expression: see section 17
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
trachea
strong strong
regionalstrong expression: see section 10 11
axial skeleton
strong strong
regionalstrong expression: see section 11 moderate expression: see section 10 12 13 14
nucleus pulposus
moderate moderate
regionalmoderate expression: see section 10
scapula
strong strong
regionalstrong expression: see section 01 02 03 19
sternum
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 04 05 06 13 14
tail mesenchyme
strong strong
regionalstrong expression: see section 08 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7471
Entity Detected:Htra1, HtrA serine peptidase 1 ( MGI:1929076)
Sequence:sense strand is shown

>T7471
CGGACGCGTGGGGTCCAGCCCGTGGCCGTCGGAGTCGTCATGCAGTCCCTGCGTACCACGCTCCTGTCTT
TGCTACTGCTGCTGCTAGCGGCTCCTTCCTTGGCGTTGCCGTCGGGGACCGGCCGCTCGGCCCCAGCTGC
CACCGTCTGTCCCGAGCACTGCGATCCCACCCGCTGCGCCCCGCCGCCCACGGACTGCGAGGGTGGCCGC
GTCCGCGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCGCTCGAGGGTGCAGCGTGCGGCCTGCAGGAGG
GTCCCTGCGGCGAGGGGCTGCAATGCGTAGTGCCCTTCGGGGTGCCGGCCTCGGCCACAGTACGACGGCG
CGCACAGGCCGGCCTGTGCGTGTGTGCCAGCAGCGAGCCGGTGTGTGGTAGCGACGCCAAGACCTACACC
AACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAGAAGCTTCGCCAGCCGCCGGTCATCGTCCTGC
AGCGCGGCGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGTCATAAGTACAACTTTATTGCTGA
TGTGGTGGAGAAGATCGCCCCGGCTGTGGTTCACATTGAACTATATCGCAAGCTTCCTTTCTCGAAGAGG
GAGGTGCCAGTGGCCAGTGGGTCAGGATTCATCGTATCGGAGGATGGACTGATTGTGACAAATGCTCACG
TGGTCACCAACAAAAACCGGGTCAAGGTTGAGCTGAAGAATGGAGCTACCTATGAAGCCAAAATCAAGGA
TGTGGATGAAAAGGCGGACATTGCGCTTATCAAGATTGACCACCAGGGAAAGCTGCCAGTCCTGCTGCTC
GGCCGCTCCTCAGAGCTGAGACCTGGAGAATTTGTAGTTGCCATTGGAAGCCCCTTTTCTCTTCAAAACA
CAGTCACCACTGGGATCGTCAGCACCACCCAGCGAGGCGGCAAAGAGCTGGGACTTCGGAACTCCGATAT
GGACTACATTCAGACAGACGCTATCATCAATTATGGAAATTCCGGAGGCCCGTTAGTAAACCTGGATGGC
GAGGTGATTGGGATTAACACCTTGAAGGTGACGGCGGGCATCTCCTTCGCAATTCCATCCGATAAGATAA
AAAAGTTCTTGACACAGTCCCACGATCGACAGGCCAAAGGGAAAGCTGTCACCAAGAAGAAGTATATTGG
GATCCGAATGATGTCGCTCACATCTAGCAAAGCCAAAGAGCTGAAGGACCGTCACCGAGACTTCCCGGAT
GTGCTCTCTGGGGCATATATCATTGAAGTCATTCCTGACACCCCGGCAGAAGCCGGAGGGCTCAAGGAAA
ATGACGTCATCATCAGCATCAACGGACAGTCTGTGGTCACTGCCAATGACGTCAGCGATGTCATCAAAAA
GGAGAACACCCTGAACATGGTTGTCCGCAGGGGCAATGAAGACATTGTGATTACCGTGATTCCTGAAGAA
ATCGACCCCTAGGCAGAGGCAGGAGCCAGACTTCATGTTTCCCTCAAAGACTCCAGGGGATGGCGCATGA
GAACTGAGCTGGTGACGCAGGACACCCGGGACCTTTGTCCAACATTTTGCTTGTTCAGGGAACACCCCTG
CCGACAGAATCCTTCTTGATAGTTTGCGGGCAAAACAAATGTAATGTTGCAGGTGCACAGGCAGAAGTCC
TGCCTTCTGTATGCTATGTATGCAGCGTGCTTTTCCTTACAAGCTTGGGCTGTTCCTGCTTACACAGTCA
ACATTTGTCCCTTCCCTTAGCCTAAGTTGTCTGACTAATGCAGTTGACGGATGCGTAGGCAAGAGGAAGG
TCCTCGCAGCCATGGTCTTGGGCGTGTTTACACTTTTCCTCCGAGTCGGCACTCAGAGGAATTTGATGCC
CCGAGACCACAGGTGGGTGACCCTGGCTTCTGAGATGGCCAAAAATGCCTCTTAGGAATCCTGGAACCCG
GAGTACAGTGACTTTGAGTTTCAGCTATTAAAATACTTCTTCATACGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4235912 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235912 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19622 same embryo
 EMAGE:19621 same embryo
 EMAGE:19623 same embryo
 EMAGE:19619 same embryo
 EMAGE:19624 same embryo
 EurExpress:euxassay_005061 same experiment
 MGI:4825486 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS