EMAGE mouse gene expression spatial database

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EMAGE:19732

Cacybp calcyclin binding protein ( MGI:1270839)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006213. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006213_01	euxassay_006213_02	euxassay_006213_03	euxassay_006213_04

euxassay_006213_05	euxassay_006213_06	euxassay_006213_07	euxassay_006213_08	euxassay_006213_09

euxassay_006213_10	euxassay_006213_11	euxassay_006213_12	euxassay_006213_13	euxassay_006213_14

euxassay_006213_15	euxassay_006213_16	euxassay_006213_17	euxassay_006213_18	euxassay_006213_19

euxassay_006213_20	euxassay_006213_21	euxassay_006213_22	euxassay_006213_23	euxassay_006213_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19732_wholemount_strong.wlz

19732_wholemount_moderate.wlz

19732_wholemount_weak.wlz

19732_wholemount_possible.wlz

19732_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19732_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 21 22 23 24 moderate expression: see section 10 11 12 13 14 15 16 20

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 16 17 18

brain

moderate

homogeneous

moderate expression: see section 24

forebrain

moderate

homogeneous

moderate expression: see section 24

diencephalon

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 10 20 21 22 23

telencephalon

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 05 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

cervical ganglion

moderate

regional

moderate expression: see section 09 16

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 19 20 weak expression: see section 09 10 11 12 15 16 17 18

mandible

moderate

regional

moderate expression: see section 03 04 05 06 21 22 23 weak expression: see section 07 08 09 10 11 12 16 17 18 19 20

lower jaw molar

weak

regional

weak expression: see section 08 09 10 18 19

maxilla

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 09 10 11 12 16 17 18 19 20

upper jaw molar

weak

regional

weak expression: see section 08 09 10 18 19

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 10 15 16 17 18 19

orbito-sphenoid

strong

regional

strong expression: see section 05 06 07 22 23 moderate expression: see section 24 weak expression: see section 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T630
Entity Detected:	Cacybp, calcyclin binding protein ( MGI:1270839)
Sequence:	sense strand is shown >T630 TCCTCGAGCCTGTTGGCCTACTGGAGCTCCGGCCTCGGGTCTGACGCGCGACGGCATGGCTTCCGTTTTG GAAGAGTTGCAGAAAGACCTAGAAGAGGTCAAAGTATTGCTGGAAAAGTCCACTAGGAAAAGACTACGTG ATACTCTTACAAGTGAAAAGTCCAAGATTGAGACGGAACTCAAGAACAAGATGCAACAGAAGTCGCAGAA GAAACCAGAACTTGATAATGAAAAGCCAGCTGCTGTGGTTGCTCCTCTTACAACAGGATACACCGTGAAA ATCAGTAATTATGGATGGGATCAGTCAGATAAGTTTGTGAAAATCTACATTACCTTGACTGGCGTCCATC AGGTGCCCACTGAGAACGTGCAGGTGCACTTCACAGAGAGGTCATTTGATCTTCTGGTAAAAAACCTCAA TGGCAAGAATTACTCCATGATTGTGAACAATCTTTTGAAACCTATCTCTGTGGAAAGCAGTTCAAAAAAA GTCAAGACTGATACAGTAATTATTCTATGTAGAAAGAAAGCAGAAAACACAAGATGGGACTACTTAACAC AGGTGGAAA
Notes:	The probe template was PCR amplified from IMAGE:1886450 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1886450 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19734 same embryo
	EMAGE:19737 same embryo
	EMAGE:19736 same embryo
	EMAGE:19735 same embryo
	EMAGE:19733 same embryo
	EurExpress:euxassay_006213 same experiment
	MGI:4823588 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source