EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19776

Zfp663 zinc finger protein 663 ( MGI:2685854)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006315. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006315_01	euxassay_006315_02	euxassay_006315_03	euxassay_006315_04

euxassay_006315_05	euxassay_006315_06	euxassay_006315_07	euxassay_006315_08	euxassay_006315_09

euxassay_006315_10	euxassay_006315_11	euxassay_006315_12	euxassay_006315_13	euxassay_006315_14

euxassay_006315_15	euxassay_006315_16	euxassay_006315_17	euxassay_006315_18	euxassay_006315_19

euxassay_006315_20	euxassay_006315_21	euxassay_006315_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19776_wholemount_strong.wlz

19776_wholemount_moderate.wlz

19776_wholemount_weak.wlz

19776_wholemount_possible.wlz

19776_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior abdominal wall musculature

moderate

regional

moderate expression: see section 03 04

cranial musculature

moderate

spotted

moderate expression: see section 01 02 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22

pectoral girdle and thoracic body wall musculature

moderate

spotted

moderate expression: see section 02 07 08 09 10 17 18 19 20 21 22

pelvic girdle musculature

moderate

spotted

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 06 07 08 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 14 15 16

spinal cord

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 11

cervical ganglion

moderate

regional

moderate expression: see section 05 06 13

thoracic ganglion

weak

regional

weak expression: see section 08 09

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

not examined

regional

not examined expression: see section 01

not examined

regional

not examined expression: see section 01 02 03 04 22

retina

moderate

regional

moderate expression: see section 02 03 04 22

tongue

weak

spotted

weak expression: see section 12 13 14 15

genital tubercle of male

moderate

regional

moderate expression: see section 13 14 15 16

lung

moderate

spotted

moderate expression: see section 16 17 18 19 weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8513
Entity Detected:	Zfp663, zinc finger protein 663 ( MGI:2685854)
Sequence:	sense strand is shown >T8513 GATACCAGGCATGAACCAGCAGCTACAGACCAGTCCTTTCTGCAGGAAGATACCAGGCACCAACCAGCAG CTGTAGGTCAATCCTGAAGAAACTGCCAGGCTTCCTAACCGGCCTAAGGCAAGGCTGCAGAAGCAGCAAG CTGCCGCAGGAGCCTCACAAGCAGTTCTCGGCTACCCTCTAAGCCTTTACTCATGCTACAGCTGCAGTTG CCCAGAGCTTGCTGTGCACAGACAACGACTGGAGAGATGGCTCGGAGGGTGAATTGCCCACCGAGATGCA TAAGGACCTGAGTACAGAACTCCAGCACCCATGTTTAAAAGCCAGGGACCAGTGTTGTTCGAAGATGTGT CTGTGGTCTTCACTCAGAAGGAGTGGCAGCTGCTGGATGCTGCCCAGAGGCACCTGTACAGGGAAGTGAT GCTGGAGACCTACAGGCACCTACGAGCAGTGGGGCACAATGTAACCAAACCTGAGCTGATATGCAAGCTG GAGCAAGGAGAAAGCCCTTGGGAACCCTCAGGCCACAGCCTCTCAGAAGTCCAGAGGGCCGATACCATGA CAACAAATGAAGAAAACGGAGGCAGATATTTGAGTCAAATCTTTATCAGTGATGAAACACTGACTAAGAG AAGAAGCAAGGATCTAAAAGAAATCGTTCATCTGGCTACAGACCCAGAGGCCTCAAGAGCAATACGTCAG AAGTGTTGCTCAATGGAAGCAAGCTTGGAAAACATCACAGGATTAGTTACTGGCAACAGAAACCACACAA CAAAAAAGTTGGATGGTTTAGGTGGCTCCAGGTCGCCTGATGAAAGATCTCATATAGGATGTAAAAACTG TGAACGTGACCCAAAGAAAAAATCCCACAGTCCTATGGAGGGCCCTACTCAACAGCAGAAGACATCGCGT TTGGAGCAACTTCTTGAATATCAAAATTGTGGGAAGACCTCACACACAAAGGCAGCCTCTGTCATACACC AGACAGTTCACGCAGGACAGGATAGCAAATACACACAAGCCACTGCCCACAAGATAAGGTTCCAGTCTTT TCTAAGAACCCTACGTGAAAGGAAGAAGCAAGAGGCTAGCCAACATGGAAAAGCACTGTGTGTGAAGTCA AAGCATGAACCTGCAGGAACTCCTGTAAGGGAGAAACTCTGTGGGCGTGGCATGCTTGAGAAACCCTATA AAGAGAAATTGGATCTTTGCAGAGGTCAGAGAAGGCAGCCAGGAGAGCCTGAATGTAGTGTCAGTGAGAA TGCCATAAGCAGCTCACCTCACCTCCAAAATGAGGAACTTCATGTGGGAGAGAAAATCTGTGCCAGTGGG AACTGTGGGGGAACCCTTCCTGGAAAGCCACGTCTTTCTCAGAACCGGAAAACTTGCACAGCAGAAAAAC CCAAGTGTGCAGACACACAAACAGCCTTAAAGACCTCACATCAGATCCTACATCCGAGACTCAGCACAAG GGAGAAAACCCCCAAAGGGGACGAGTGTGGGAGGTCTCCGCTCCTCTCCCGCAAGGAACTGAGATGCAGT CAATACGGGAGACCATCCTTGGGGGAGAAACCGGAGTGTGAGGGAAATGAGAAGCCGGACCATAGTGAAA ACCAGAGCCTTTGCAAAGAACAGAAAGCCCATGAGGGCTGCAAGTGTGAGGAAGGCTGTCCCAAGACTGC AAGCCTTCCTCAACACCAGAGCTCACACACAGAGAAAAAAACCTATGCATGCAATGAATGCAACAAGTCC TTCCTGGTGAAGTCACACCTCACCGAACACCAGAGAACTCACACGGGAGAGAAACCCTATGAATGTAAAG AGTGTGGAAAGTCATTTTGCCAAAAATACGCCCTCACGGTACACCAGAGAACTCACACGGGAGAGAAACC ATACAAGTGCAACGAATGTGGGAAAACTTTCTGCGTGAAATCCAACCTTACCCAACACCAAAGGACTCAC ACGGGGGATAAGCCGTACAAGTGCAGCGAATGCTGGAGGTCGTTCTGTGTCAAGTCCAACCTCGTCGTGC ACCAGAGAACTCACACTGGAGAGAAGCCCTACAGATGTCTCGAGTGTGGGAAGACCTTCTATGAGAAATC TGCCCTCACAAAACATCGGCGGATTCACACAGGGGAAAAACCTTATGAATGCGAGGAATGCAAAAAAAGT TTCAGCCAGAGGTCGGCCCTTACCAAGCACCAAAGAAAAACGCACAAGAAGAAAACACCCACCGGCAGCC CGCGCGGGCAGAAATCTGAACCAACCAGCGAAGCTCACTGAATAGTGACAGCACGAAAGAGCAAGACGCT GCCATGGCAACTCGCATCGTTGAGAACTCCGCCATCAGATTATACATGATAGACGCTAGAGAATTAATGC AGGGCTAAAAGCATAAAAATATTTCAAACATGTGAATCCTTTCAACAGGTATTCAACATTGTCGAAGGAG AAAGAAATGATTTTAGTGAATGCGAAGAACAAATGTCCTTGTGGAAATATTGTCTAACTGGAAGTTATAT TTCAGATGTGATGCAGAACTCATTACAGGACAGATAACAGAACACCATCTGTTCTTCAGGCAGAATGAGG CAAGGCTCTTTCATAACAGTGATAAGTGAGGCTTTACTGATCCAGTTGTAGCAAAGTCATCTTTTGCCGG TCCTGCTTCATTCTGTTCCTCATGTTTTCTCCATTTCCTTCAAGGGCATTGACGGAGATAAAGGAACAAG AGTGGAAAAGACCAAAAGCAAATTAGAGGAATCTAAGCCCTGGGTGTGAAAAGATCCTAGGAACAGCTAT CGAAAACGGCACAAACAAGAGTCTATGTTAAACGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTGCGTG CGCGTGCACACGTGCGTGCGTGTGAGCATGTGAATGTGATACCATGATGAGTTAAAAACGATGTCCAGCG GCCAGCAAGGGCTCAATGTTAAAAGCCCCTGCTCCACATGCCTCGTGACCTCTGTTTGATTCCTGGAACC CTTGTAAAGGTGGAATGAGAGAACTCACACCAGACATTTGTCCTCTGAGCCCACACATGCACAGGGGCAC ACACACACACACACACACACACACACACACTAATAATAATAAGTAAAATAAAAAATTTAAACGAAACCCC TAAATCTAACCTAACATCAGGCCATTTGTAATGAGAAGAAGAGAAAGAGGAGGAAGAAGAACACGCTATA AATTCAGTTGAAAGGGGGATTTGAAAGCTAGAAGATAGCAAAAGTATATATTTTTAAATTAACTCAATAA AGTGTGGCTTTGGAATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5705933 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5705933 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19777 same embryo
	EMAGE:19774 same embryo
	EMAGE:19772 same embryo
	EMAGE:19773 same embryo
	EMAGE:19775 same embryo
	EurExpress:euxassay_006315 same experiment
	MGI:4825084 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source