EMAGE mouse gene expression spatial database

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EMAGE:19804

Dsp desmoplakin ( MGI:109611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006313. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006313_01	euxassay_006313_02	euxassay_006313_03	euxassay_006313_04

euxassay_006313_05	euxassay_006313_06	euxassay_006313_07	euxassay_006313_08	euxassay_006313_09

euxassay_006313_10	euxassay_006313_11	euxassay_006313_12	euxassay_006313_13	euxassay_006313_14

euxassay_006313_15	euxassay_006313_16	euxassay_006313_17	euxassay_006313_18	euxassay_006313_19

euxassay_006313_20	euxassay_006313_21	euxassay_006313_22	euxassay_006313_23	euxassay_006313_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19804_wholemount_strong.wlz

19804_wholemount_moderate.wlz

19804_wholemount_weak.wlz

19804_wholemount_possible.wlz

19804_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19804_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19 20 weak expression: see section 10

pancreas

weak

regional

weak expression: see section 07 08 09 13 14

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 19 20 21 22 23 24

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

conjunctival sac

strong

regional

strong expression: see section 01 02 03 04 05 22 23 24

naris

strong

regional

strong expression: see section 13 17 moderate expression: see section 12 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18

naso-lacrimal duct

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23 24

heart atrium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

pharynx epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16

tongue epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

stomach

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06

rectum

weak

regional

weak expression: see section 11 12

midgut

moderate

regional

moderate expression: see section 05 06 weak expression: see section 07 08 09 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 11 12 13 16 17

lower jaw molar

strong

regional

strong expression: see section 07 08 19

oral epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 12 13 16 17

upper jaw molar

strong

regional

strong expression: see section 07 08 19

kidney calyx

weak

regional

weak expression: see section 05 06 07 08 14 15 16 17

kidney pelvis

weak

regional

weak expression: see section 15 16

seminiferous cord

weak

regional

weak expression: see section 03 04 05 06 17 18

urethra of male

strong

regional

strong expression: see section 11 12

lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

trachea epithelium

strong

regional

strong expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35002
Entity Detected:	Dsp, desmoplakin ( MGI:109611)
Sequence:	sense strand is shown >T35002 GATTCCTCCAAGCTGGTATTTGATGGGCTGAGGAAGAAGGTGACGGCAATGCAGCTCTATGAGTGCCAGC TGATCGACAAGACGACGCTAGACAAGCTGTTGAAGGGGAAGAAGTCAGTGGAGGAGGTGGCCTCAGAGAT CCAGCCTTTCCTGCGCGGCGCAGGCGCCATCGCCGGGGCTTCCGCTTCTCCCAAGGAAAAGTACTCTTTG GTGGAGGCCAAGAGAAAGAAATTCATCACCCCAGAATCCACTGTCATGCTTCTGGAGGCCCAGGCAGCCA CCGGTGGTATCATCGATCCCCATCGGAATGAGAAGCTCACTGTGGACAATGCCGTAGCCCGGGATCTCAT CGACTTCGACGACCGCCAACAGATCTACACAGCGGAGAAAGCCATCACTGGTTTCGATGACCCGTTTTCG GGCAAGACGGTCTCTGTTTCAGAAGCCATCAAGAAGAATTTGATCGACAGAGAAACCGGAATGCGCCTGC TGGAAGCCCAGCTTGCTTCAGGGGGTGTAGTTGATCCAGTCAACAGTGTCTTCTTGCCAAAAGATGTCGC CTTGGCCCGTGGGCTGATTGATAGAGATTTGTATCGCTCCTTGAACGATCCCCGAGATAGTCAGAAGAAC TTCGTGGATCCAATCACCAAGAAGAAGGTCAGCTACATGCAGCTGAGAGAAAGGTGCAGAATCGAACCCC ACACGGGTCTGCTCCTGCTGTCAGTACAGAAGAGAAGCATGTCCTTCCAGGGAATCAGACAACCCGTGAC CGTCACCGAACTGGTTGATTCTGGCATATTGAGACCGTCCACTGTAAACGAACTGGAATCCGGTCAGATT TCCTATGATGAGGTTGGGGAGAGAATCAAGGACTTCCTCCAGGGTTCAAGCTGCATAGCAGGCATATATA ATGAGACCACTAAGCAGAAGCTGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50010. Forward Primer - name:050010_F_cDNA_Dsp, sequence:GATTCCTCCAAGCTGGTATTTG; Reverse Primer - name:050010_N_SP6_cDNA_Dsp, sequence:CCCAGCTTCTGCTTAGTGGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19801 same embryo
	EMAGE:19802 same embryo
	EMAGE:19805 same embryo
	EMAGE:19800 same embryo
	EMAGE:19803 same embryo
	EurExpress:euxassay_006313 same experiment
	MGI:4824402 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source