EMAGE mouse gene expression spatial database

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EMAGE:19884

A430107O13Rik RIKEN cDNA A430107O13 gene ( MGI:2444814)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009303. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009303_01	euxassay_009303_02	euxassay_009303_03	euxassay_009303_04

euxassay_009303_05	euxassay_009303_06	euxassay_009303_07	euxassay_009303_08	euxassay_009303_09

euxassay_009303_10	euxassay_009303_11	euxassay_009303_12	euxassay_009303_13	euxassay_009303_14

euxassay_009303_15	euxassay_009303_16	euxassay_009303_17	euxassay_009303_18	euxassay_009303_19

euxassay_009303_20	euxassay_009303_21	euxassay_009303_22	euxassay_009303_23	euxassay_009303_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19884_wholemount_strong.wlz

19884_wholemount_moderate.wlz

19884_wholemount_weak.wlz

19884_wholemount_possible.wlz

19884_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19884_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm mesenchyme

moderate

regional

moderate expression: see section 01 02 23

hand

moderate

regional

moderate expression: see section 01 02 03 04 05 23 24

foot

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19 20 21 22

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 20 21 22 23 24

upper leg mesenchyme

moderate

regional

moderate expression: see section 01 20 21 22 23 24

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

dermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 20 21 22 23 24

diencephalon meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord meninges

moderate

regional

moderate expression: see section 09 10 11 13 14

ear

moderate

regional

moderate expression: see section 01

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

renal capsule

strong

regional

strong expression: see section 04 05 06 07 08 13 14 15 16 17

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

right lung

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35819
Entity Detected:	A430107O13Rik, RIKEN cDNA A430107O13 gene ( MGI:2444814)
Sequence:	sense strand is shown >T35819 TAACCACATACAGCCAAACTGCTATCCCCCTTTTTTATGTTAAAAGCCGTGAATGCTAGCAGCAAAGTGG CAATTTAAAACTGAACGAAAGGAAGCAGCAGTTCTACAACATAAAAGGCTGGGAGCGGGATGGCTTTAAA ACCGGAGGGAAAATGAGAAACTGCTAGGACAACCTGGCCGGCGCCCAGCTGTGCAACAGGCACTTCGCGT TAAATCGACTGCGTCTTCGGCTGGTTTCTGCCTCCAGACCCGCTTTACGGTGCTTTCTTTTTGCCCCAAA AGAGGAGACAAGCTGAACTCCTGACTTTAAGAATGTTGTGGAAGTAAGGCATTACCGGAAGGAACTCTTG GTAGCTGGGGATGCATTCGGATGATTTCTTATAACTATCTTTATTTTGACATCTTAAAAAAAAATCTGGC CAGTCACTTCTTACTGTCCTTCATTTAAGGGCTATTAACCACCTCTGACCGCTGGGTCCTATGAATCTTT GGCATGAACCTTCGGAAAGTTTTCAAGCGGAGATGGGGCGAACTTGATTGGAATTGTGTAAAAAGAAGAC AGATGTGTGTTTTTTTCACACTGACAACGTGGCCAGGGGCCAAGTGATCCTGGACTGGTCATGGTCTGTC GACCCGGGTTCCTTTGTCGCCGGCGATTTTGCCCCCGACCTTTCCTGGTGGGTTTGGTGGTGGCGATCTG TCTCTTTTACCAGACCCTGACACTCCGAGGGACTAAGAAGATTGCAGCGGACGTCTCTGGCACTGTCCCA CACACACCCTCTCAAACTCAGGCTAGCAGATGTGAAGAAGGATACCGGCAGGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79253. Forward Primer - name:079253_F_cDNA_A430107O13Rik, sequence:TAACCACATACAGCCAAACTGC; Reverse Primer - name:079253_N_SP6_cDNA_A430107O13Rik, sequence:GTCCTGCCGGTATCCTTCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19889 same embryo
	EMAGE:19886 same embryo
	EMAGE:19888 same embryo
	EMAGE:19885 same embryo
	EMAGE:19887 same embryo
	EurExpress:euxassay_009303 same experiment
	MGI:4822830 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source