EMAGE mouse gene expression spatial database

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EMAGE:19913

Adamtsl2 ADAMTS-like 2 ( MGI:1925044)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009340. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009340_01	euxassay_009340_02	euxassay_009340_03	euxassay_009340_04

euxassay_009340_05	euxassay_009340_06	euxassay_009340_07	euxassay_009340_08	euxassay_009340_09

euxassay_009340_10	euxassay_009340_11	euxassay_009340_12	euxassay_009340_13	euxassay_009340_14

euxassay_009340_15	euxassay_009340_16	euxassay_009340_17	euxassay_009340_18	euxassay_009340_19

euxassay_009340_20	euxassay_009340_21	euxassay_009340_22	euxassay_009340_23	euxassay_009340_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19913_wholemount_strong.wlz

19913_wholemount_moderate.wlz

19913_wholemount_weak.wlz

19913_wholemount_possible.wlz

19913_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19913_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

weak

regional

weak expression: see section 01 02 03

upper leg muscle

weak

regional

weak expression: see section 02 03 04 05 19 20

hand

weak

regional

weak expression: see section 01 02 09 24

foot

weak

regional

weak expression: see section 05 06 07 08 10 22 23 24

lower leg rest of mesenchyme

weak

regional

weak expression: see section 03 04 05

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14

heart valve

moderate

regional

moderate expression: see section 11 12 13

tongue muscle

weak

regional

weak expression: see section 12 13 14 15 16 17

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

right lung

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

tail mesenchyme

weak

regional

weak expression: see section 12 13 14 15 16 17 18

tail paraxial mesenchyme

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35844
Entity Detected:	Adamtsl2, ADAMTS-like 2 ( MGI:1925044)
Sequence:	sense strand is shown >T35844 GCTATGATGGTGTTGAAGTGGATGACAGCTACTGCGATGCCCTCACCCGTCCTGAGCCTGTCCACGAATT CTGCGCTGGGAGGGAGTGCCAGCCCAGGTGGGAGACCAGCAGCTGGAGTGAGTGCTCACGTACCTGTGGT GAGGGCCATCAGTTCCGCATCGTGCGCTGCTGGAAGATGCTGTCCCCTGGATTTGACAGCTCCGTGTACA GTGACCTCTGTGAAGCCACGGAGGCTGTCCGGCCGGAGGAACGCAAGACATGCCGCAACCCAGCCTGCGG ACCACAGTGGGAGATGTCTGAGTGGTCAGAGTGCACTGCCAAGTGTGGAGAGCGCAGTGTAGTGACCAGA GACATCCGCTGTTCCGAGGACGAGAAACTGTGTGACCCCAGCACCAAGCCTGTGGGAGAGAAGAACTGCA CTGGGCCTCCCTGTGACCGCCAGTGGACTGTCTCGGACTGGGGACCGTGCAGTGGGAGCTGTGGACAGGG CCGCACTATCAGGCATGTGTACTGTAAGACCAGCGATGGACGGGTAGTTCCCGAGTCTCAGTGCCAGACA GAGACAAAGCCTCTGGCTATCCACCCCTGTGGGGACAAGAACTGCCCAGCACACTGGCTGGCTCAAGACT GGGAAAGGTGCAATACCACCTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76362. Forward Primer - name:076362_F_cDNA_A930008K15Rik, sequence:GCTATGATGGTGTTGAAGTGGA; Reverse Primer - name:076362_N_SP6_cDNA_A930008K15Rik, sequence:ACAGGTGGTATTGCACCTTTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19917 same embryo
	EMAGE:19915 same embryo
	EMAGE:19914 same embryo
	EMAGE:19916 same embryo
	EMAGE:19912 same embryo
	EurExpress:euxassay_009340 same experiment
	MGI:4822992 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source