EMAGE mouse gene expression spatial database

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EMAGE:19996

Shb src homology 2 domain-containing transforming protein B ( MGI:98294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009465. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009465_01	euxassay_009465_02	euxassay_009465_03	euxassay_009465_04

euxassay_009465_05	euxassay_009465_06	euxassay_009465_07	euxassay_009465_08	euxassay_009465_09

euxassay_009465_10	euxassay_009465_11	euxassay_009465_12	euxassay_009465_13	euxassay_009465_14

euxassay_009465_15	euxassay_009465_16	euxassay_009465_17	euxassay_009465_18	euxassay_009465_19

euxassay_009465_20	euxassay_009465_21	euxassay_009465_22	euxassay_009465_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19996_wholemount_strong.wlz

19996_wholemount_moderate.wlz

19996_wholemount_weak.wlz

19996_wholemount_possible.wlz

19996_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19996_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 10 11 12 13 14 15 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 15 16 17

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

moderate

regional

moderate expression: see section 08 10 11 12 13 14 15 16 17 weak expression: see section 05 06 07

neural retina

weak

regional

weak expression: see section 02 03 04 05 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 17 moderate expression: see section 10 11 12 13 15 16 18 19

lower jaw molar

moderate

regional

moderate expression: see section 08 20

upper jaw molar

moderate

regional

moderate expression: see section 08 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37017
Entity Detected:	Shb, src homology 2 domain-containing transforming protein B ( MGI:98294)
Sequence:	sense strand is shown >T37017 TGATGACTACTCCGATCCCTTTGATGCCAAGAGCGACCTCAAGAGCAAAGCAGGAAAGGGTGAGAGCGCT GGCTACATGGAGCCCTACGAAGCCCAGAGGATCATGACAGAATTCCAGAGGCAAGAGAGCGTCCGGTCAC AGCACAAAGGCATCCAGTTGTACGACACCCCGTACGAGCCGGAAGGCCAGAGCGTGGACTCAGACTCAGA GAGCACTGTGAGCCTTCGGCTGCGGGAGAGCAAGCTACCCCAGGATGACGACAGGCCTGCTGATGAGTAC GACCAGCCGTGGGAATGGAACCGGGTCACCATCCCCGCCCTGGCAGCCCAGTTTAACGGCAATGAAAAGC GGCAGTCCTCCCCATCACCTTCTCGGGACCGACGGCGCCAGCTCCGTGCCCCGGGAGGAGGCTTCAAGCC CATCAAGCACGGGAGCCCCGAGTTCTGTGGGATTCTGGGGGAGAGAGTGGATCCCACCATCCCACTGGAG AAACAGATATGGTATCACGGGGCCATCAGCAGAAGTGATGCTGAGAACCTGCTGCGACTCTGTAAGGAGT GCAGCTACCTTGTCCGGAACAGCCAGACCAGCAAACATGACTACTCCCTCTCCCTGAAGAGTAACCAGGG CTTCATGCACATGAAACTGGCCAAGACCAAAGAGAAGTATGTTCTGGGTCAGAACAGCCCCCCTTTTGAC AGCGTCCCGGAAGTCATCCACTACTACACCACCAGGAAGCTGCCCATCAAAGGGGCCGAGCACTTGTCCC TCCTCTACCCAGTGGCTGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77867. Forward Primer - name:077867_F_cDNA_Shb, sequence:TGATGACTACTCCGATCCCTTT; Reverse Primer - name:077867_N_SP6_cDNA_Shb, sequence:CACAGCCACTGGGTAGAGGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19993 same embryo
	EMAGE:19998 same embryo
	EMAGE:19994 same embryo
	EMAGE:19995 same embryo
	EMAGE:19997 same embryo
	EurExpress:euxassay_009465 same experiment
	MGI:4828048 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source