EMAGE mouse gene expression spatial database

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EMAGE:20077

Sars seryl-aminoacyl-tRNA synthetase ( MGI:102809)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012234. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012234_01	euxassay_012234_02	euxassay_012234_03	euxassay_012234_04

euxassay_012234_05	euxassay_012234_06	euxassay_012234_07	euxassay_012234_08	euxassay_012234_09

euxassay_012234_10	euxassay_012234_11	euxassay_012234_12	euxassay_012234_13	euxassay_012234_14

euxassay_012234_15	euxassay_012234_16	euxassay_012234_17	euxassay_012234_18	euxassay_012234_19

euxassay_012234_20	euxassay_012234_21	euxassay_012234_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20077_wholemount_strong.wlz

20077_wholemount_moderate.wlz

20077_wholemount_weak.wlz

20077_wholemount_possible.wlz

20077_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20077_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15

pons mantle layer

weak

regional

weak expression: see section 07 16

facial vii ganglion

weak

regional

weak expression: see section 04 05 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 08 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 16 17

ventral grey horn

weak

regional

weak expression: see section 10 11 14

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 15 16

orbito-sphenoid

weak

regional

weak expression: see section 04 05 19 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36990
Entity Detected:	Sars, seryl-aminoacyl-tRNA synthetase ( MGI:102809)
Sequence:	sense strand is shown >T36990 TAATGATGAGGACGCAGACAACAAAGTAGAACGTATTTGGGGAGATTGTACAGTCAGGAAGAAGTATTCC CATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGAGCCGTGGTGGCTGGTAGTCGGG GGTACTTCCTGAAGGGGCCCCTGGTGTTCCTGGAGCAGGCGCTTATCCAATATGCACTGCGTACCTTGGG GAGTCGGGGCTACACTCCAATCTACACCCCCTTCTTCATGAGGAAAGAGGTCATGCAGGAAGTGGCCCAG CTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGCGAAAAGTCAGATGACAACTCCT ATGACGAGAAATACTTGATTGCCACCTCAGAGCAGCCCATCGCGGCTCTGCACCGGGACGAGTGGCTGCG GCCAGAGGATCTGCCCATCAAGTACGCTGGCCTCTCCACCTGCTTTCGTCAGGAAGTGGGCTCGCATGGC CGTGACACCCGTGGTATCTTCCGAGTCCATCAGTTTGAGAAGATTGAGCAGTTTGTGTACTCATCGCCCC ATGACAATAAGTCGTGGGAGATGTTTGATGAGATGATCGCCACCGCAGAAGAATTCTACCAGTCTTTGGG GATCCCTTACCACATTGTGAATATTGTCTCAGGCTCCTTGAATCACGCTGCCAGTAAGAAGCTCGACCTG GAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89231. Forward Primer - name:089231_F_cDNA_Sars1, sequence:TAATGATGAGGACGCAGACAAC; Reverse Primer - name:089231_N_SP6_cDNA_Sars1, sequence:CCTCCAGGTCGAGCTTCTTAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20078 same embryo
	EMAGE:20079 same embryo
	EMAGE:20080 same embryo
	EMAGE:20081 same embryo
	EMAGE:20082 same embryo
	EurExpress:euxassay_012234 same experiment
	MGI:4827871 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source