EMAGE mouse gene expression spatial database

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EMAGE:20094

6720463M24Rik RIKEN cDNA 6720463M24 gene ( MGI:1924994)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012253_01	euxassay_012253_02	euxassay_012253_03	euxassay_012253_04	euxassay_012253_05

euxassay_012253_06	euxassay_012253_07	euxassay_012253_08	euxassay_012253_09	euxassay_012253_10

euxassay_012253_11	euxassay_012253_12	euxassay_012253_13	euxassay_012253_14	euxassay_012253_15

euxassay_012253_16	euxassay_012253_17	euxassay_012253_18	euxassay_012253_19	euxassay_012253_20

euxassay_012253_21	euxassay_012253_22	euxassay_012253_23	euxassay_012253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20094_wholemount_strong.wlz

20094_wholemount_moderate.wlz

20094_wholemount_weak.wlz

20094_wholemount_possible.wlz

20094_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20094_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14 15

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 17 18 19

vibrissa

weak

regional

weak expression: see section 05 21

hypothalamus ventricular layer

weak

regional

weak expression: see section 11 12 14 15

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 12 13 14

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 15 16 weak expression: see section 10

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 14 15 16 17 18 weak expression: see section 02 03 04 05 06 07 08 09 10 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 weak expression: see section 09 18

lower jaw incisor

weak

regional

weak expression: see section 11 12 14 15

upper jaw incisor

weak

regional

weak expression: see section 10 11 14 15

renal cortex

weak

regional

weak expression: see section 09 10 11 17 19 20

temporal bone

weak

regional

weak expression: see section 23

orbito-sphenoid

weak

regional

weak expression: see section 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30398
Entity Detected:	6720463M24Rik, RIKEN cDNA 6720463M24 gene ( MGI:1924994)
Sequence:	sense strand is shown >T30398 CGAGCATCAGGAGTCCGTACATAGATGGCTGCTCGCCAATCAAAAACTGGTCTCCTCGGAGACTGAGAGG TGGCCCTCAGTGTTTGTCCTCCCTGGTCCGCATACCTTTTACCCTTGAGGCTCACAGTGAAGATGAAGAG GCGGACGTTTCCTGCACAGGCGCGGCTCCACTCAGCACAAATGCTTGTGGTGAGCCTCGAGTTGTCACTG CCATGTCTGTTACACAGAGTCACTCTGGTATTGCTGAGAAAGAGCGAGCAGTGCTGGACGATGCGGAAAG TGAGAGGGAAAATGACACCGTGGATATGGTCGATCCTACAGACACAGTAGCTGAAAGCACGTGGATAAAA GAGCCAGTGGATGATGGGAATTCACCCATGACGGATTCCGCGAGTGGAATTGCCTTCAGTATTGAAAACT CTCATATGTGCATGTCGCCTCTTGCAGAAAGCAGTGTCCTGCCTTATGAAAGCAGTGCTATCCAGATGAA TAGTGACTATAACACACAGACTTGTGTAAGTAATATCACTGATATTGTTGGGACAGAAAGATATTGCAAA GAAAATGTCACACATACAAATGTTCCAGTGCCCTTTGAAGTGGAAATGAAATCTCAAGTTAATAACGTAA CGCCCGGCCACACAGCGCAGCGGTGCTGGATGAAATCTCCGCGTCCATCTCAGTGCAGCCGCCCGTAGTC TCAAGACCAAAGACGGGTGCTCAGCGAGTTAGTGTGATGCTGATGGGTGCCTGCTTCACGTACTGTGCCT ACTTCCCTCAGTAATGTGAAAGGCTGCAAAGGAAATTGGTGCTAGGACCAGAAACTCCAAGTTGTTAAAT TATGTTTTTTTTAAATCAATAAATATTTCTATATTTGCTTCAAGTGGCTTGTTTAACAAGTAAATTATGG CAGACCTCATGGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6336038), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58638. Forward Primer - name:058638_F_IRAV103_b11_6720463M24Rik, sequence:CGAGCATCAGGAGTCCGT; Reverse Primer - name:058638_R_SP6_IRAV103_b11_6720463M24Rik, sequence:AAGCCATGAGGTCTGCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20093 same embryo
	EMAGE:20095 same embryo
	EMAGE:20092 same embryo
	EMAGE:20096 same embryo
	EurExpress:euxassay_012253 same experiment
	MGI:4822790 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source