EMAGE mouse gene expression spatial database

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EMAGE:20115

Ppp6r2 protein phosphatase 6, regulatory subunit 2 ( MGI:1918724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012428. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012428_01	euxassay_012428_02	euxassay_012428_03	euxassay_012428_04

euxassay_012428_05	euxassay_012428_06	euxassay_012428_07	euxassay_012428_08	euxassay_012428_09

euxassay_012428_10	euxassay_012428_11	euxassay_012428_12	euxassay_012428_13	euxassay_012428_14

euxassay_012428_15	euxassay_012428_16	euxassay_012428_17	euxassay_012428_18	euxassay_012428_19

euxassay_012428_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20115_wholemount_strong.wlz

20115_wholemount_moderate.wlz

20115_wholemount_weak.wlz

20115_wholemount_possible.wlz

20115_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20115_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14

telencephalon

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

metencephalon alar plate

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

metencephalon basal plate

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

midbrain

moderate

homogeneous

moderate expression: see section 04 05 08 09 10 11 12 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 13 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 14 15 16 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 14

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 06 08 12 13 weak expression: see section 07

neural retina

moderate

regional

moderate expression: see section 01 19 20 weak expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31305
Entity Detected:	Ppp6r2, protein phosphatase 6, regulatory subunit 2 ( MGI:1918724)
Sequence:	sense strand is shown >T31305 GGCTTGTGGCATTGATCCATCCCCACCAGGATGAAGATAGGCAGTCAAATGCTTCTCAGGCACTCTGTGA CATCATCAGGCTGGGCAGAGACCAGGGCAGTCAGCTGCAGGAGACTGTGGAGCCAGACCCCCTCCTCATA ACATTGGAGTCGCAGGACTGTGTGGAGCAGCTTCTGAAGAACATGTTTGATGGGGATCAGACGGAGAGCT GCCTGGTCAGTGGGATGCAGGTGCTGCTTGCCTTGCTGGAGCCAAGGCGTGTTGGAACAGAAGGCTTGGT GGACTCCTTTTCTCAGGGACTGGAAAGGTCACACTCCGTCAGCAGCAGCATCCTTCGTGGCATCGAGCCA TGGCTGAAGAATTTCCATCAGCTCCTCCTCAACCCACCAAAGAAAAAAGCAATCCTGACCACTATTGGTG TGTTAGAGGAGCCCCTGGGGAATGCTCGTCTTCATGGTGCACGCCTCATGGCTGCACTGCTGCACACAAA CACACCTGGCATCAACCAGGAGCTCTGCCGGCTCAACACCATGGACTTACTGCTGGACTTGTTCTTTAAG TACACCTGGAATAACTTTTTGCACCTCCAAGTGGAACTATGCATAGCCGCTATTCTTTCCCACGCTGCTC GGGAGGAGCAGGCAGAAGCCAGTGGATCCGATGGCAAGGTGGAGCCTCTGCAGGGCAGTGGAGATGGGAA TGGGAAGCTGGAGACCACCCCATCCATCACCAGCCCTCCTGAGAACACAATGGTGACCCATCTGTTCCAG AAGTGCTGCCTGGTACAGAGGATCCTGGAAGCCTGGGAAGCCAATGACCACACACAGGCAGCAGGTGGTA TGAGACGTGGGAATATGGGCCACCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4011449), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22271. Forward Primer - name:022271_F_IRAV51-54_D20_8430411H09Rik, sequence:GGCTTGTGGCATTGATCC; Reverse Primer - name:022271_R_SP6_IRAV51-54_D20_8430411H09Rik, sequence:GAGGTGGCCCATATTCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20113 same embryo
	EMAGE:20117 same embryo
	EMAGE:20112 same embryo
	EMAGE:20116 same embryo
	EMAGE:20114 same embryo
	EurExpress:euxassay_012428 same experiment
	MGI:4827868 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source