EMAGE mouse gene expression spatial database

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EMAGE:20127

Sorbs2 sorbin and SH3 domain containing 2 ( MGI:1924574)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012430. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012430_01	euxassay_012430_02	euxassay_012430_03	euxassay_012430_04

euxassay_012430_05	euxassay_012430_06	euxassay_012430_07	euxassay_012430_08	euxassay_012430_09

euxassay_012430_10	euxassay_012430_11	euxassay_012430_12	euxassay_012430_13	euxassay_012430_14

euxassay_012430_15	euxassay_012430_16	euxassay_012430_17	euxassay_012430_18	euxassay_012430_19

euxassay_012430_20	euxassay_012430_21	euxassay_012430_22	euxassay_012430_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20127_wholemount_strong.wlz

20127_wholemount_moderate.wlz

20127_wholemount_weak.wlz

20127_wholemount_possible.wlz

20127_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20127_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 05 20 21

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 04

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 05 06 20 21 weak expression: see section 19

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 04

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 05 06 20 21 weak expression: see section 19

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 04

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 05 06 07 20 21 weak expression: see section 19

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 05 06 20 21

tarsus

weak

regional

weak expression: see section 04 22 23

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 17 18 19

thyroid gland

moderate

regional

moderate expression: see section 11 12 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13

cerebral cortex mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23

olfactory cortex mantle layer

strong

regional

strong expression: see section 10 11 14 15 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

rest of cerebellum mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17 18 19 20 21 22

pons mantle layer

weak

regional

weak expression: see section 08 18

otic capsule

moderate

regional

moderate expression: see section 07 08 09 17 18 19 20 weak expression: see section 10

cochlea

moderate

regional

moderate expression: see section 07 08 09 18 19 20 21

nasal septum

moderate

regional

moderate expression: see section 11 12 13 14 15 16

heart ventricle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

tongue muscle

moderate

regional

moderate expression: see section 14 weak expression: see section 11 12 13 15 16

metanephros

weak

regional

weak expression: see section 07 08 09 10 16 17 18 19 20

thyroid cartilage

weak

regional

weak expression: see section 12 13 14 15

exoccipital bone

moderate

regional

moderate expression: see section 01 04 21 22 23 weak expression: see section 02 03

temporal bone petrous part

moderate

regional

moderate expression: see section 01 04 05 21 22 23 weak expression: see section 02 03

viscerocranium

strong

regional

Expression in the turbinate bone.

axial skeleton tail region

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31311
Entity Detected:	Sorbs2, sorbin and SH3 domain containing 2 ( MGI:1924574)
Sequence:	sense strand is shown >T31311 TTCCTGCTTTCTGCCCTGAACTTTGAAATGCCTGTGTAAGGAATCAAAATGAACATGATTGTACTTTTTA AAAGTGAAATAATTAAGGAAGAAAGAAAGAGGAAAAAAAGAGAGAGAGACTCTCCAGGAGACTGTCTGGT GTCATGACTGAATCGCCACCTCTCTGGAAGGTGTACCGTCCTCAGGAAGCCAGAAGGTTTTGTTTTGTTT TTTTCTATGGTTCTCATTTTCACCCTCAGCTTGGCAGTTCATTTTCCTTTCACGAGTTTGCCTAGTGCTC TGGTTTACACTATATGACAACTATACTTCAGCTGTTCTTTGCCTGCACTTATTATATACATTGTTTCATG CACAGTTATTACAAATCAGGAGCGTTTGGAAAGTTCACTGGGACGAGTGTGATTTTTTGTTGAGTGCATT ACATCTTCACATGGGGGCGGGGAGGTCTTTTCCCTGCCCATGTTTTTTTGTGCTTATTAGAGGTGCAAAC AGCGAGCGATTAAGAACTGTTGGTCAACTGTTACGAGACTATAAAAGGCTTAGCCACACTACGGGACAAA TTGTGTTGTGAATTCGCATTGCTGTTTTGTGTACTAAAACGTAACCATTGGCATTGTGAACAACAAGCTC TTAGTGGTGTATTTACCTGTTAAAACTTAAAAGCAGTGTTAGTGAGTTAGGCAGAGAGATGAAAGTAATA CAGTCAGTAAGTTTCACTGCGTGCACACTGACACGTTTGAGATTGTAGGAGGACATATAATTCATATGCT ATGTTGTACATTTTATGGAAATATAAAAGAATCCCACGTTATTTTATAGAGTACTTCAGGAGCATCCTAA GTGTTAAGGCTGGCTTTAGCAAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4017890), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22396. Forward Primer - name:022396_F_IRAV51-54_F04_9430041O17Rik, sequence:TTCCTGCTTTCTGCCCTG; Reverse Primer - name:022396_R_SP6_IRAV51-54_F04_9430041O17Rik, sequence:TCCTTGCTAAAGCCAGCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20125 same embryo
	EMAGE:20128 same embryo
	EMAGE:20124 same embryo
	EMAGE:20126 same embryo
	EMAGE:20123 same embryo
	EurExpress:euxassay_012430 same experiment
	MGI:4828378 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source